Two central pattern generators from the crab, Cancer borealis, respond robustly and differentially to extreme extracellular pH
- Brandeis University, United States
Figures
Figure 1
Preparations and circuit diagrams.
(A) Schematic of the stomatogastric nervous system preparation. Extracellular electrodes were placed in vaseline wells (gray circles) drawn around nerves of interest. An example extracellular nerve …
Figure 2
Robust pyloric rhythm activity across pH.
(A) Example recordings from a stomatogastric ganglion experiment with an acid-first protocol. Intracellular recordings of the PD and LP neurons and extracellular recordings of the lvn are shown. …
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Figure 2—source data 1
Numerical data represented in panel B, C, and D of Figure 2.
- https://doi.org/10.7554/eLife.41877.004
Figure 3 with 2 supplements
Variability of pyloric rhythm activity at extreme pH.
(A) Two additional stomatogastric ganglion experiments displaying 3 s of intracellular PD and extracellular lvn recordings. Horizontal lines indicate a reference membrane potential of −40 mV; …
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Figure 3—source data 1
Numerical data represented in panel C, D, E, and F of Figure 3 and and panel A, B, and C of Figure 3—figure supplement 2.
- https://doi.org/10.7554/eLife.41877.008
Figure 3—figure supplement 1
Statistical analysis of the effects of pH on the pyloric rhythm.
The main effects of acid and base protocols on four measures of the activity of the pyloric rhythm were assessed. Univariate Type III Repeated-Measures Analysis of Variance (ANOVA) tests were …
Figure 3—figure supplement 2
Variability of pyloric rhythm activity at control pH.
(A) Pyloric rhythm frequency, (B) number of PD spikes per burst, and (C) PD duty cycle were calculated for each CG preparation for the last 8 min in control pH 7.8. Violin plots show the KDE …
Figure 4 with 1 supplement
Intracellular characteristics of semi-isolated pyloric neurons, PD and LP.
Several characteristics of the PD and LP neurons in the presence of picrotoxin (PTX) were measured for the last minute of each pH condition. (A) Example intracellular recordings of PD and LP neurons …
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Figure 4—source data 1
Numerical data represented in panel B, C, D, E, F, and G of Figure 4.
- https://doi.org/10.7554/eLife.41877.011
Figure 4—figure supplement 1
Statistical analysis of the effects of pH on semi-isolated PD and LP neurons.
The main effects of acid and base protocols on six measures of the activity of semi-isolated LP and PD neurons were assessed. Univariate Type III Repeated-Measures Analysis of Variance (ANOVA) tests …
Figure 5
Rhythmic gastric-like activity upon recovery from extreme acid.
(A) 20 min of recording are shown from an example experiment where the ganglion had become silent at pH 5.5 and began recovering rhythmic activity in control pH 7.8 saline. Intracellular recordings …
Figure 6
Robust and variable cardiac rhythm activity across pH.
(A) Two example cardiac ganglion experiments with an acid-first protocol. Each colored box displays 12 s of extracellular recordings of the trunk taken from the last minute of each pH condition. …
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Figure 6—source data 1
Numerical data represented in panel B, C, and D of Figure 6.
- https://doi.org/10.7554/eLife.41877.014
Figure 7 with 2 supplements
Characteristics of cardiac rhythm activity across pH.
(A) Four states were defined to characterize cardiac rhythm activity. Examples of activity for each state are given. (B) Stacked bars give the mean fraction of time that all 15 preparations spent in …
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Figure 7—source data 1
Numerical data represented in panel B, C, D, and E of Figure 7 and panel A, B, and C of Figure 7—figure supplement 2.
- https://doi.org/10.7554/eLife.41877.018
Figure 7—figure supplement 1
Statistical analysis of the effects of pH on the cardiac rhythm.
The main effects of acid and base protocols on four measures of the activity of the cardiac rhythm were assessed. Univariate Type III Repeated-Measures Analysis of Variance (ANOVA) tests were …
Figure 7—figure supplement 2
Variability of cardiac rhythm activity at control pH.
(A) Cardiac rhythm frequency, (B) number of LC spikes per burst, and (C) LC duty cycle were calculated for each CG preparation for the last 8 min in control pH 7.8. Violin plots show the KDE …
Figure 8 with 1 supplement
Rhythmicity of the cardiac and pyloric rhythms compared across pH.
(A) Mean fraction of time that both the pyloric (blue) and cardiac (green) rhythms displayed normal activity is plotted as a function of pH. Differences between the activity of the two rhythms were …
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Figure 8—source data 1
Numerical data represented in panel A and B of Figure 8.
- https://doi.org/10.7554/eLife.41877.021
Figure 8—figure supplement 1
Statistical analysis of the differential effects of pH on the pyloric and cardiac rhythms.
The main effects of ganglion and pH and their interaction during both acid and base protocols on the fraction of time rhythmic of both the pyloric and cardiac rhythms were assessed. Multivariate …
Additional files
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Transparent reporting form
- https://doi.org/10.7554/eLife.41877.022
