Dashed line indicates median incidence observed in TD (white marker) and ASD (black marker) populations (values from Figure 1—source data 1).
Incidence of sleep problems in Autism Spectrum Disorder (ASD) compared to typically developing (TD) individuals.
Survey of the literature, regardless of age. Median, first and third quartile values, age range, and included references reported for each phenotype.
The rows represent the vigilance states of wakefulness (top), NREM sleep (middle), and REM sleep (bottom). (A) Time spent in wakefulness and sleep shown as percentage of recording time per hour. …
Source data and summary statistics used for generating plots for Figure 2.
Statistical results for Figure 2 and Figure 2—figure supplement 1. numDF, numerator degrees of freedom. denDF, denominator degrees of freedom.
(A) Average number of state-specific bouts per hour shown in 6 hr blocks. (B) Average bout duration (min) per hour shown in 6 hr blocks. Repeated measures ANOVA with posthoc pairwise comparisons …
(A) Normalized NREM delta (δ; 0.5–4 Hz) power during recovery sleep post-sleep deprivation. The cross-hatched bar on the x-axis denotes the 5 hr sleep deprivation period and the open bar denotes the …
Source data and summary statistics used for generating plots for Figure 3.
Statistical results for Figure 3, Figure 3—figure supplement 1, and Figure 3—figure supplement 2.
numDF, numerator degrees of freedom. denDF, denominator degrees of freedom.
The rows represent the vigilance states of wakefulness (top), NREM sleep (middle), and REM sleep (bottom). (A – B) Time in state shown as percentage of recording time per hour comparing baseline and …
(A) Average number of state-specific bouts per hour (difference scores) shown in 7 hr (remaining light period) and 6 hr blocks. (B) Average bout duration (min) per hour (difference scores) shown in …
RNA-seq study of gene expression from prefrontal cortex obtained from adult male Shank3ΔC and wild type mice either under control homecage conditions (HC) or following 5 hr of sleep deprivation …
Genes differentially expressed between Shank3∆C vs. wild type mice.
RNA-seq study of gene expression from prefrontal cortex tissue obtained from adult male Shank3ΔC and wild type (WT) mice, either under control homecage conditions (HC) or following 5 hr of sleep deprivation (SD). N = 5 mice per group. False discovery rate (FDR) < 0.1.
Volcano plot of differential expression (−log10 P-value versus log2 fold change) caused by sleep deprivation in wild type animals (HC5 vs SD5). Genes with an FDR < 0.05 are highlighted in black. …
Representative actograms and periodograms for two wild type and two Shank3∆C mice. Mice were entrained to a 12:12 hr light:dark cycle (LD, 559 ± 4 : 0 ± 0 lux) for two weeks prior to 3 weeks …
Mice were entrained to a 12:12 hr light:dark cycle (LD, 559 ± 4 : 0 ± 0 lux) for two weeks prior to 3 weeks constant darkness (DD, 0 ± 0 lux). Gray shading is representative of the dark period. (A) …
Repeated measures ANOVA with posthoc pairwise comparisons using Sidak correction. Values are means ± SEM for wild type (n = 10) and Shank3ΔC (n = 10). *p<0.05, difference from wild type.
Total Sleep Time (%) | Wild Type | Shank3ΔC | p-value |
---|---|---|---|
Light Period | 62.90 ± 1.57 | 62.96 ± 1.65 | 0.980 |
Dark Period | 28.72 ± 2.41 | 21.16 ± 2.54 | 0.045* |
Functional annotation and clustering analysis was performed using DAVID (https://david.ncifcrf.gov) and functional information was obtained from the following databases: GO (Biological process and …
Shank3ΔC vs. Wild Type | |
---|---|
Homecage | Sleep Deprivation |
Up-regulated | Up-regulated |
Cholesterol Metabolism: Hmgcr, Insig1 | Potassium Ion Transport: Kcnv1, Kcnk1, Kcnk2 |
Transcription: Jun, Fosl2, Nfil3, Stat4 | Dephosphorylation: Dusp10, Dusp3, Ptprj |
Neuron Projection Development: Cntn1, Ntrk2, Reln, Sema3a | |
Down-regulated | Down-regulated |
MAPK Signaling: Mapk3 (ERK1), Elk1 | GnRH Signaling: Mapk1 (ERK2), Elk1, Mapk11 (p38) |
Circadian Rhythms: Per3, Tef, Hlf | Circadian Rhythms: Per3, Nr1d1 (REV-ERBα), Tef, Hlf, Prkab2 (AMPK), Bhlhe41 (DEC2) |
Transcription: Tef, Hlf | Sodium Ion Transport: Slc6a15, Slc22a4, Slc24a4 |
Functional annotation clustering analysis of genes in Figure 4—source data 1.
Summary of wheel-running behavior measured for each week of 12:12 hr light dark cycle (LD, 559 ± 4 : 0 ± 0 lux) or constant darkness (DD, 0 ± 0 lux) for both wild type and Shank3∆C mice. A linear …
Wild Type | Shank3∆C | ||
---|---|---|---|
Period (h) | |||
LD Week 2 | 24.0 ± 0.0 | 24.0 ± 0.0 | |
LD Week 3 | 24.0 ± 0.0 | 23.9 ± 0.1 | |
DD Week 1 | 23.9 ± 0.1 | 23.7 ± 0.1 | |
DD Week 2 | 23.7 ± 0.1 | 20.3 ± 3.4 | |
DD Week 3 | 23.5 ± 0.2 | 25.8 ± 1.5 | |
Alpha (h) | |||
LD Week 2 | 10.0 ± 0.4 | 10.7 ± 0.4 | |
LD Week 3 | 10.3 ± 0.4 | 10.7 ± 0.5 | |
DD Week 1 | 10.5 ± 0.2 | 9.0 ± 0.4 | |
DD Week 2 | 10.4 ± 0.4 | 8.2 ± 0.7 | |
DD Week 3 | 9.0 ± 1.0 | 9.9 ± 1.0 | |
Activity (rev/day)* | |||
LD Week 2 | 33985.2 ± 565.9 | 23258.2 ± 861.2 | |
LD Week 3 | 40306.7 ± 1257.1 | 25365.1 ± 964.0 | |
DD Week 1 | 37942.6 ± 795.4 | 23027.7 ± 669.6 | |
DD Week 2 | 38250.7 ± 431.1 | 16820.7 ± 1302.5 | |
DD Week 3 | 38586.3 ± 231.1 | 6961.5 ± 383.9 |
Results of genotype x time interaction analysis for LD week three through DD week 3.
Linear mixed-effects ANOVA for LD week three through DD week 3. The model was fitted using genotype, time, and their interaction as fixed effects and the individual mice labeled as random effect. There is a significant effect of genotype x time for activity (p<0.0001) and no significant effect for genotype x time or genotype for period and alpha. Significance at p<0.05. numDF, numerator degrees of freedom. denDF, denominator degrees of freedom.
Reagent type (species) or resource | Designation | Source or reference | Identifiers |
---|---|---|---|
Gene (Mus musculus) | Shank3; SH3 and multiple ankyrin repeat domains 3 | NA | ENTREZ_ID: 58234 |
Gene (Mus musculus) | Per3; period circadian clock 3 | NA | ENTREZ_ID: 18628 |
Gene (Mus musculus) | Hlf; hepatic leukemia factor | NA | ENTREZ_ID: 217082 |
Gene (Mus musculus) | Tef; thyrotroph embryonic factor | NA | ENTREZ_ID: 21685 |
Gene (Mus musculus) | Nr1d1; nuclear receptor subfamily 1, group D, member 1; | NA | ENTREZ_ID: 217166 |
Gene (Mus musculus) | Bhlhe41; basic helix-loop-helix family, member e41; | NA | ENTREZ_ID: 79362 |
Strain, strain background (Mus musculus) | Shank3ΔC; Shank3tm1.1Pfw/J | Kouser et al., 2013; The Jackson Laboratory | RRID:IMSR_JAX:018398 |
Commercial assay or kit | RNAeasy kit | Qiagen | 74104 |
Commercial assay or kit | High Sensitivity RNA Analysis Kit | Advanced Analytical Technologies | DNF-472 |
Commercial assay or kit | High Sensitivity NGS Fragment Analysis Kit | Advanced Analytical Technologies | DNF-474 |
Commercial assay or kit | KAPA Library Quantification Kit | Kapabiosystems | KK4824 |
Commercial assay or kit | TruSeq Stranded mRNA Library Prep Kit | Illumina | RS-122–2101 |
Software, algorithm | HiSeq Control Software version (v. 2.2.68) | Illumina | 15073358 Rev A |
Software, algorithm | bcl2fastq (v. 2.17.1.14) | R/Bioconductor | SCR_015058 |
Software, algorithm | Rsubread (v. 1.26.1) | R/Bioconductor | SCR_016945 |
Software, algorithm | NOISeq (v.2.22.1) | R/Bioconductor | SCR_003002 |
Software, algorithm | RUVSeq (v. 1.12.0) | R/Bioconductor | SCR_006263 |
Software, algorithm | edgeR (v. 3.20.9) | R/Bioconductor | SCR_012802 |
Software, algorithm | R package nlme (v. 3.5.0) | CRAN | SCR_015655 |
Software, algorithm | R package mgcf (v. 3.5.0) | CRAN | |
Software, algorithm | R package pheatmap (v. 1.0.10) | CRAN | SCR_016418 |
Software, algorithm | Database for annotation, visualization, and integrated discovery (v 6.7) | DAVID; https://david.ncifcrf.gov | |
Software, algorithm | VitalRecorder | Kissei Comtec | 3, 0, 0, 0 |
Software, algorithm | SleepSign for Animal | Kissei Comtec | 3, 0, 0, 812 |
Software, algorithm | Wheel manager | Med Associates Inc | SOF-860 |
Software, algorithm | ClockLab (v. 3.5.0) | Actimetrics | 6.0.50 |
Results of genotype x time interaction analysis for 5 weeks of LD.
Linear mixed-effects ANOVA for LD week 1–5. The model was fitted using genotype, time, and their interaction as fixed effects and the individual mice labeled as random effect. There is a significant effect for genotype x time in activity (p<0.0001), a significant effect of genotype for period (p=0.047), and no significant effect for genotype x time or genotype for alpha. Significance at p<0.05. numDF, numerator degrees of freedom. denDF, denominator degrees of freedom.
Detailed report of statistical analysis including R code to reproduce the RNA-seq and circadian wheel running analysis.
Wheel-running behavior in Shank3∆C mice across 5 weeks of continuous light:dark (LD) cycles.
Summary of wheel-running behavior measured for each week of 12:12 hr light:dark cycles for both wild type and Shank3∆C mice. A linear mixed-effects ANOVA was used to estimate the contributions of genotype and the interaction between genotype and time for period, alpha, and wheel-running activity for LD week 1 through 5. There is no significant interaction effect for genotype x time for period (p=0.41), but there is a significant effect of genotype (p=0.047, indicated by *). There is no significant interaction effect for genotype x time for alpha (p=0.43), but there is a significant effect of time (p=0.004, indicated by †). There is a significant interaction effect (indicated by vertical bar) for genotype x time for activity (p<0.0001). See Source data 1 for details. Values are means ± SEM for wild type (n = 8) and Shank3∆C (n = 8) mice.