1. Neuroscience

Shank3 modulates sleep and expression of circadian transcription factors

  1. Ashley M Ingiosi
  2. Hannah Schoch
  3. Taylor Wintler
  4. Kristan G Singletary
  5. Dario Righelli
  6. Leandro G Roser
  7. Elizabeth Medina
  8. Davide Risso
  9. Marcos G Frank  Is a corresponding author
  10. Lucia Peixoto  Is a corresponding author
  1. Washington State University, United States
  2. Consiglio Nazionale della Ricerche, Italy
  3. University of Fusciano, Italy
  4. University of Padova, Italy
  5. Weill Cornell Medicine, United States
https://doi.org/10.7554/eLife.42819
Share this article
Cite this article
  1. Ashley M Ingiosi
  2. Hannah Schoch
  3. Taylor Wintler
  4. Kristan G Singletary
  5. Dario Righelli
  6. Leandro G Roser
  7. Elizabeth Medina
  8. Davide Risso
  9. Marcos G Frank
  10. Lucia Peixoto
(2019)
Shank3 modulates sleep and expression of circadian transcription factors
eLife 8:e42819.
https://doi.org/10.7554/eLife.42819
  2. Download BibTeX
  3. Download .RIS

Abstract

Autism Spectrum Disorder (ASD) is the most prevalent neurodevelopmental disorder in the United States and often co-presents with sleep problems. Sleep problems in ASD predict the severity of ASD core diagnostic symptoms and have a considerable impact on the quality of life of caregivers. Little is known, however, about the underlying molecular mechanisms of sleep problems in ASD. We investigated the role of Shank3, a high confidence ASD gene candidate, in sleep architecture and regulation. We show that mice lacking exon 21 of Shank3 have problems falling asleep even when sleepy. Using RNA-seq we show that sleep deprivation increases the differences in prefrontal cortex gene expression between mutants and wild types, downregulating circadian transcription factors Per3, Bhlhe41, Hlf, Tef, and Nr1d1. Shank3 mutants also have trouble regulating wheel-running activity in constant darkness. Overall, our study shows that Shank3 is an important modulator of sleep and clock gene expression.

https://doi.org/10.7554/eLife.42819.001

eLife digest

Autism spectrum disorder (ASD) is the most common neurodevelopmental disorder in the United States. People with ASD tend to have difficulties with communication and social interactions, restricted interests, and may repeat certain behaviors. They also often struggle to fall or stay asleep. Sleep deprivation may exacerbate other symptoms of the disorder. This makes life more difficult for both the person with ASD and their caregivers. Scientists do not yet know what causes sleep difficulties in people with ASD.

Unraveling the complex genetics that underlie ASD may help scientists better understand ASD-related sleep difficulties. One possible genetic culprit for sleep difficulties in ASD is a gene called SHANK3. Patients with an ASD-associated condition called Phelan-McDermid syndrome are often missing the SHANK3 gene. They also often have sleep problems.

Now, Ingiosi, Schoch et al. show that both patients with Phelan-McDermid syndrome and mice with a mutation in the Shank3 gene have problems falling asleep. Using a registry that collects genetic and sleep information on people with Phelan-McDermid syndrome, Ingiosi, Schoch et al. found that people who are missing SHANK3 frequently have trouble falling asleep and wake up many times each night. Mice missing part of the Shank3 gene also had difficulty falling asleep, even after they have been deprived of sleep.

Mice naturally have a daily pattern of sleep and activity. This 24-hour activity cycle is maintained by an internal circadian clock. In mice missing part of Shank3, the circadian clock genes are not turned on correctly. These genes were less active in mice missing Shank3, and this difference worsened with lack of sleep. These mice also ran less on a wheel than typical mice when kept in total darkness, even though the pattern of activity did not change. The experiments suggest that Shank3 controls sleep, likely through its effects on circadian clock genes. Learning more about what causes these sleep problems may help scientists develop ways to improve sleep in people with ASD and Phelan-McDermid syndrome.

https://doi.org/10.7554/eLife.42819.002

Introduction

Autism Spectrum Disorder (ASD) is the most prevalent neurodevelopmental disorder in the United States (diagnosed in 1 in 59 children [Baio et al., 2018]). The core symptoms of ASD include social and communication deficits, restricted interests, and repetitive behaviors (American Psychiatric Association, 2013). In addition, several studies show that individuals with ASD report a variety of co-morbid conditions including sleep problems and altered circadian rhythms (Glickman, 2010). It is estimated that 40–80% of the ASD population experience sleep disorders that do not improve with age (Johnson et al., 2009). More specifically, people with ASD have problems falling and staying asleep (Hodge et al., 2014). A recent study showed that sleep problems co-occur with autistic traits in early childhood and increase over time, suggesting that sleep problems are an essential part of ASD (Verhoeff et al., 2018). Indeed, sleep impairments are a strong predictor of the severity of ASD core symptoms as well as aggression and behavioral issues (Cohen et al., 2014; Tudor et al., 2012). Although a great number of studies documented sleep problems in ASD, little is known about the underlying molecular mechanisms.

To better understand the mechanisms underlying sleep problems in ASD, we need animal models that closely recapitulate sleep phenotypes observed in clinical populations. The study of genetic animal models of ASD, in which a genetic abnormality that is known to be associated with ASD is introduced, has provided valuable insight into the molecular mechanisms underlying ASD (de la Torre-Ubieta et al., 2016). These models include Fragile X syndrome, 16p11.2 deletion syndrome, cortical dysplasia-focal epilepsy (CDFE) syndrome, and mutations in neuroligins, neurexins, and shank genes among others. However, sleep research in animal models of ASD is limited and has not yet revealed the underlying mechanisms of sleep issues associated with ASD. Studies using a fly model of Fragile X syndrome reported an increase in sleep which is in contrast to what is observed in the clinical population (Bushey et al., 2009). The opposite phenotype was reported in a Fragile X mouse model, displaying instead an age-dependent reduction in activity during the light phase (i.e the mouse subjective night) (Boone et al., 2018). Neuroligin 1 knockout mice spend more time asleep (El Helou et al., 2013), but mice with mutations in Neuroligin 2 spend less time asleep and more time awake (Seok et al., 2018). Mice with a missense mutation in Neuroligin 3 show normal sleep behavior (Liu et al., 2017), but rats with a deletion mutation in Neuroligin 3 spend less time in non-rapid eye movement (NREM) sleep than wild type rats (Thomas et al., 2017). Mutant rat models of CDFE syndrome show longer waking periods while the mutant mice show fragmented wakefulness (Thomas et al., 2017). Mice carrying a 16p11.2 deletion syndrome sleep less than wild type mice, but only males are affected (Angelakos et al., 2017). More importantly, issues with sleep onset, the most prominent feature of sleep problems in ASD patients, have not been evaluated in animal models of ASD.

In this study, we examined sleep in Phelan-McDermid syndrome (PMS) patients with SHANK3 mutations and in a mutant mouse with a deletion in Shank3 exon 21 (Shank3ΔC). PMS is a syndromic form of ASD characterized by gene deletions affecting the human chromosomal region 22q13.3 (Phelan and McDermid, 2012), particularly the neuronal structural gene SHANK3. Individuals with PMS have high rates of intellectual disability, difficulties in communication and motor function, and approximately 84% fit the core diagnostic criteria for ASD (Soorya et al., 2013). There is also a high rate of sleep problems in PMS (Bro et al., 2017). Mice with mutations in Shank3 recapitulate multiple features of both ASD and PMS (Bozdagi et al., 2010; Dhamne et al., 2017; Jaramillo et al., 2017; Jaramillo et al., 2016; Kouser et al., 2013; Peça et al., 2011; Speed et al., 2015), including cognitive impairment, deficits in social behavior, and impaired motor coordination. We show that PMS patients have trouble falling and staying asleep similar to what is observed in the general ASD population. We also show that Shank3ΔC mice sleep less than wild type mice when sleep pressure is high, have reduced sleep intensity (using an accepted electroencephalographic (EEG) metric), and have delayed sleep onset following sleep deprivation. To identify molecular mechanisms underlying sleep changes in Shank3ΔC mice, we carried out genome-wide gene expression studies. We previously showed that genome-wide gene expression analysis is a valuable approach to understand the molecular mechanisms underlying the detrimental effects of sleep deprivation (Gerstner et al., 2016; Vecsey et al., 2012). In this study, we found that sleep deprivation sharply increases the differences in gene expression between Shank3ΔC mutants and wild type mice, downregulating circadian transcription factors Per3, Bhlhe41 (Dec2), Hef, Tlf, and Nr1d1 (Rev-erbα). We also show that Shank3ΔC mice are unable to sustain wheel-running activity in constant darkness. Overall, these studies demonstrate that Shank3 is an important modulator of sleep that may exert its effect through the regulation of circadian transcription factors. Our findings may lead to a deeper understanding of the molecular mechanisms underlying sleep problems in ASD. This may one day lead to the development of successful treatments or interventions for this debilitating comorbidity.

Results

Phelan-McDermid syndrome patients have problems falling and staying asleep

Recent studies suggest that sleep problems may be present in a substantial number of PMS patients and may be an important factor for caregivers' well-being (Bro et al., 2017). We obtained genetic and sleep questionnaire data from the Phelan-McDermid Syndrome International Registry (PMSIR) to estimate the frequency and age of onset of sleep problems in PMS individuals carrying a SHANK3 deletion. In parallel, we surveyed the clinical literature to estimate the prevalence of sleep problems in ASD (Andersen et al., 2008; Cotton and Richdale, 2006; Gail Williams et al., 2004; Giannotti et al., 2008; Giannotti et al., 2006; Krakowiak et al., 2008; Liu et al., 2006; Miano et al., 2007; Paavonen et al., 2008; Polimeni et al., 2005; Richdale and Prior, 1995; Tani et al., 2003; Thirumalai et al., 2002; Wiggs and Stores, 2004) and typically developing populations (Anders and Eiben, 1997; Baweja et al., 2013; Bixler et al., 2009; Hysing et al., 2013; Leger et al., 2012; Loessl et al., 2008; Lozoff et al., 1985; Lumeng and Chervin, 2008; Ohayon et al., 2000; Pallesen et al., 2008; Patzold et al., 1998; Sadeh et al., 2000). Figure 1 shows that PMS patients have trouble falling asleep and experience multiple night awakenings starting at about 5 years of age. Those difficulties translate to reduced time asleep particularly during adolescence. Although total sleep time seems to improve in adulthood for PMS patients, that improvement is accompanied by an increase in parasomnias. Problems falling and staying asleep persist regardless of age. The frequency of problems falling and staying asleep in PMS patients is similar to what is observed in the general ASD population and much higher than in typically developing individuals (Figure 1—source data 1).

Figure 1
Download asset Open asset
Increased incidence of sleep problems reported in individuals with Phelan-McDermid syndrome (PMS) compared to typically developing (TD) individuals.

Dashed line indicates median incidence observed in TD (white marker) and ASD (black marker) populations (values from Figure 1—source data 1).

https://doi.org/10.7554/eLife.42819.003
Figure 1—source data 1

Incidence of sleep problems in Autism Spectrum Disorder (ASD) compared to typically developing (TD) individuals.

Survey of the literature, regardless of age. Median, first and third quartile values, age range, and included references reported for each phenotype.

https://doi.org/10.7554/eLife.42819.004

Shank3ΔC mice sleep less during the dark phase and show reduced sleep intensity

To determine if Shank3ΔC mice have deficits in spontaneous sleep, undisturbed baseline EEG and electromyographic (EMG) recordings were obtained from wild type (WT) and Shank3ΔC mice. There was a significant period x genotype interaction for total sleep time (i.e. total time spent in NREM and rapid eye movement (REM) sleep) during the light (hours 1–12) and dark (hours 13–24) periods (F(1,28) = 5.198, p=0.036). Posthoc pairwise comparisons using Sidak correction showed that Shank3ΔC mice slept less than WT mice during the dark period (p=0.045; Table 1). To determine in which arousal states and hours this effect was most pronounced, we examined hourly time in state data for wakefulness, NREM sleep, and REM sleep. Repeated measures ANOVA over the full 24 hr recording period found significant time x genotype interactions for wakefulness (F(23,345) = 2.419, p<0.0001), NREM sleep (F(23,345) = 2.357, p=0.001), and REM sleep (F(23,345) = 2.175, p=0.002) (Figure 2A). Posthoc comparisons found the most pronounced differences at hour 19 when Shank3ΔC mice spent more time in wakefulness (p<0.0001) and less time NREM (p<0.0001) and REM (p=0.040) sleep (Figure 2A) marking the beginning of an overall trend for increased wakefulness during the last half of the dark period. Analysis of the sleep architecture found time x genotype interactions for bout number during REM sleep (F(3,51) = 2.832, p=0.047) and bout duration during NREM (F(1.749,29.734) = 4.077, p=0.032) and REM (F(1.897,32.243) = 6.536, p=0.005) sleep. Posthoc comparisons showed these effects were driven by differences in the last half of the dark period (hours 19–24) when Shank3ΔC mice had fewer REM bouts (p=0.003; Figure 2—figure supplement 1A) and shorter NREM (p=0.005) and REM (p=0.013) bouts (Figure 2—figure supplement 1B) than WT mice. Overall, these data show that Shank3ΔC mice spend more time awake at the end of the dark phase compared to WT mice under baseline conditions.

Figure 2 with 1 supplement see all
Download asset Open asset
Shank3∆C mice sleep less during the dark period and show altered EEG spectral power under baseline conditions.

The rows represent the vigilance states of wakefulness (top), NREM sleep (middle), and REM sleep (bottom). (A) Time spent in wakefulness and sleep shown as percentage of recording time per hour. Values are means ± SEM. Repeated measures ANOVA with posthoc pairwise comparisons using Sidak correction; *p<0.05, difference from wild type. (B) EEG spectral power normalized as a percentage of the total state-specific EEG power for the light period (left) and dark period (right) fit to smooth curves (solid lines) and expressed with 95% confidence intervals (gray and red shading). Non-overlap of the 95% confidence interval denotes statistically significant differences. The open bars on the x-axis denote the light period and the filled bars denote the dark period of the light:dark cycle. Wild type (n = 10) and Shank3∆C (n = 10) mice.

https://doi.org/10.7554/eLife.42819.005
Figure 2—source data 1

Source data and summary statistics used for generating plots for Figure 2.

https://doi.org/10.7554/eLife.42819.007
Figure 2—source data 2

Statistical results for Figure 2 and Figure 2—figure supplement 1. numDF, numerator degrees of freedom. denDF, denominator degrees of freedom.

https://doi.org/10.7554/eLife.42819.008
Table 1
Shank3ΔC mice sleep less than wild type mice during the dark period.

Repeated measures ANOVA with posthoc pairwise comparisons using Sidak correction. Values are means ± SEM for wild type (n = 10) and Shank3ΔC (n = 10). *p<0.05, difference from wild type.

https://doi.org/10.7554/eLife.42819.009
Total Sleep Time (%)Wild TypeShank3ΔCp-value
Light Period62.90 ± 1.5762.96 ± 1.650.980
Dark Period28.72 ± 2.4121.16 ± 2.540.045*

Fourier analysis of the EEG indicates that Shank3ΔC mice also sleep differently than WT mice (Figure 2B). Significant genotypic differences in spectral frequencies occur at points of non-overlaping 95% confidence intervals. During NREM sleep, power in the delta frequencies (0.5–4 Hz) was blunted and alpha frequencies (10–15 Hz) were enhanced for Shank3ΔC mice relative to WT during the light and dark periods (Figure 2B). NREM delta power is a measure of synchrony of the neural network and can be a measure of sleep intensity or depth (Achermann and Borbely, 2017). These data indicate that Shank3ΔC mice exhibit disrupted neural connectivity during NREM sleep which may suggest that Shank3ΔC mice sleep less deeply under baseline conditions.

Shank3ΔC mice have abnormal homeostatic responses to sleep deprivation

To investigate sleep homeostasis in Shank3ΔC mice we sleep deprived mutant and WT mice for 5 hr via gentle handling starting at light onset (hour 1). Sleep deprivation (SD) was effective resulting in WT and Shank3ΔC mice spending 97.37 ± 0.76% and 95.08 ± 1.08% time in wakefulness, respectively, during the 5 hr SD period (t(18) = 1.732, p=0.100). Both WT and Shank3ΔC mice showed homeostatic responses to SD (Figure 3—figure supplement 1) with reduced wakefulness and increased time spent in NREM and REM sleep during the recovery phase (Figure 3—source data 2). Sleep was also more consolidated after SD for both WT and Shank3ΔC mice during the light period, and changes in sleep architecture did not statistically differ between WT and Shank3ΔC mice (Figure 3—figure supplement 2). However, there were time x genotype interactions for wakefulness (F(18,288) = 2.025, p=0.009), NREM sleep (F(18,288) = 1.928, p=0.014), and REM sleep (F(18,288) = 1.716, p=0.036) over the SD recovery phase (hours 6–24). This effect was most pronounced near the end of the dark period where Shank3ΔC mice spent more time in wakefulness and less time in NREM and REM sleep compared to WT (Figure 3—figure supplement 1C) which is consistent with the trend seen in the baseline data (Figure 2A). Changes in NREM EEG delta power were similar between WT and Shank3ΔC mice, indicating that mutant mice accumulate sleep pressure similarly to WT mice (Figure 3A). However, Shank3ΔC mice showed a transient enhancement of NREM EEG spectral power (F(1,18) = 12.07, p=0.003) compared to WT mice in the first 2 hr post-SD (hours 6–7). This enhancement was found in the higher frequencies (3.9–19.5 Hz; Figure 3B) and resolved to baseline and WT values during hours 11–12 (F(1,18) = 0.59, p=0.454; Figure 3C). These data suggest that some aspects of the Shank3ΔC homeostatic response to sleep deprivation are abnormal.

Figure 3 with 2 supplements see all
Download asset Open asset
Shank3∆C mice take longer to fall asleep and sleep less after sleep deprivation.

(A) Normalized NREM delta (δ; 0.5–4 Hz) power during recovery sleep post-sleep deprivation. The cross-hatched bar on the x-axis denotes the 5 hr sleep deprivation period and the open bar denotes the remaining light period. Repeated measures ANOVA. (B) Normalized NREM spectra for the first 2 hr post-sleep deprivation (hours 6–7; significant from 3.9 to 19.5 Hz). One-way ANOVA with posthoc unpaired t-tests using Benjamini-Hochberg correction. (C) Normalized NREM spectra for the last 2 hr of the light period post-sleep deprivation (hours 11–12). One-way ANOVA. (D) Latency to enter NREM sleep and REM sleep after sleep deprivation. Unpaired t-test. (E) Time in NREM sleep and REM sleep for the first 2 hr post-sleep deprivation (hours 6–7). Unpaired t-test. (F) Time in NREM sleep and REM sleep for the remaining 5 hr of light period post-sleep deprivation (hours 8–12). Unpaired t-test. Values are means ± SEM for wild type (n = 10; black) and Shank3∆C (n = 10; red) mice. *p<0.05, difference from wild type.

https://doi.org/10.7554/eLife.42819.010
Figure 3—source data 1

Source data and summary statistics used for generating plots for Figure 3.

https://doi.org/10.7554/eLife.42819.013
Figure 3—source data 2

Statistical results for Figure 3, Figure 3—figure supplement 1, and Figure 3—figure supplement 2.

numDF, numerator degrees of freedom. denDF, denominator degrees of freedom.

https://doi.org/10.7554/eLife.42819.014

Remarkably, Shank3ΔC mice took longer to enter NREM sleep post-SD compared to WT (t(10.44) = −2.31, p=0.043; Figure 3D). As a consequence, Shank3ΔC mice spent less time in NREM sleep compared to WT during the first 2 hr of the recovery phase (t(13.26) = 2.85, p=0.014; Figure 3E). During the subsequent 5 hr of the light period (hours 8–12), Shank3ΔC mice spent more time in NREM sleep compared to WT (t(18) = −2.46; p=0.024; Figure 3F). Overall, our data show that Shank3ΔC mice have difficulties falling asleep despite heightened sleep pressure.

Shank3ΔC mice show downregulation of circadian transcription factors in response to sleep deprivation

We conducted a genome-wide gene expression study to investigate the molecular basis for the Shank3ΔC mouse sleep phenotype. Shank3ΔC and WT adult male mice were subjected to 5 hr of SD starting at light onset and sacrificed immediately post-SD. Additional mice from both genotypes were sacrificed at the same time of day to determine differences in gene expression under homecage conditions (HC). Prefrontal cortex was collected for all animals and subjected to RNA sequencing (RNA-seq, n = 5 per group). As expected, sleep deprivation is the greatest source of variation in the data (principal component 1), followed by the genotype effect (principal component 2; Figure 4A). Furthermore, the difference between genotypes is enhanced after sleep deprivation (Figure 4A) greatly increasing the number of differentially expressed genes between Shank3ΔC and WT mice – starting at 69 genes (HC) and doubling to 134 genes (SD) (Figure 4B, Figure 4—source data 1; false discovery rate (FDR) < 0.1). Most of the differences in gene expression following SD are not present in HC conditions.

Figure 4 with 1 supplement see all
Download asset Open asset
Sleep deprivation induces a two-fold difference in gene expression between Shank3ΔC and wild type mice.

RNA-seq study of gene expression from prefrontal cortex obtained from adult male Shank3ΔC and wild type mice either under control homecage conditions (HC) or following 5 hr of sleep deprivation (SD). N = 5 mice per group. (A) Principal component analysis of normalized RNA-seq data shows that sleep deprivation is the main source of variance in the data (first principal component, PC1) and genotype is the second (second principal component, PC2). Percent variance explained by each PC is shown on each axis. (B) Venn diagram showing the number of genes differentially expressed at FDR < 0.1 between Shank3ΔC and wild type mice in either control HC conditions or after SD. (C) Heat map of average scaled gene expression for all genes in (B). K-means clustering defined three clusters based on differences in gene expression across all comparisons. Genes belonging to the MAPK pathway and involved in circadian rhythms (see Table 2) are highlighted on the right.

https://doi.org/10.7554/eLife.42819.015
Figure 4—source data 1

Genes differentially expressed between Shank3∆C vs. wild type mice.

RNA-seq study of gene expression from prefrontal cortex tissue obtained from adult male Shank3ΔC and wild type (WT) mice, either under control homecage conditions (HC) or following 5 hr of sleep deprivation (SD). N = 5 mice per group. False discovery rate (FDR) < 0.1.

https://doi.org/10.7554/eLife.42819.017

Clustering of gene expression patterns for all genes differentially expressed between Shank3ΔC and WT mice reveals 3 groups of genes (Figure 4C). Cluster 1 contains genes that are downregulated in mutants versus WT mice under HC conditions. Genes in this cluster are also downregulated in response to SD in Shank3ΔC mice but are generally unaffected by SD in WT mice. SD seems to exacerbate the difference between genotypes, which explains why differential expression of some of the genes in cluster 1 only reach statistical significance after SD. This cluster contains the majority of the genes that are differentially expressed between genotypes. Cluster 2 contains genes that are upregulated in mutants versus WT mice under HC conditions that are also downregulated by SD in WT mice. Cluster 3 contains genes that are normally upregulated by SD. For both clusters 2 and 3, the Shank3ΔC mutation seems to dampen the response to SD.

To better understand the impact of the gene expression at the pathway level, we carried out functional annotation of the transcripts differentially expressed between Shank3ΔC and WT mice (Table 2, Table 2—source data 1). Our results reveal that 1) MAPK/GnRH signaling and circadian rhythm-associated transcripts are downregulated in Shank3ΔC mice and 2) sleep deprivation exacerbates that difference. Circadian transcription factors are particularly affected. For example, while expression of Per3, Hlf, and Tef already differs under homecage conditions, SD leads to additional genotype-specific differences in expression of Nr1d1 and Bhlhe41. All of the above mentioned circadian transcription factors belong to cluster 1 on our heat map (Figure 4C) showing downregulation in response to SD only in Shank3ΔC mice.

Table 2
Results of functional bioinformatics analysis of the genes differentially expressed between Shank3ΔCmice relative to wild type mice.

Functional annotation and clustering analysis was performed using DAVID (https://david.ncifcrf.gov) and functional information was obtained from the following databases: GO (Biological process and Molecular function), KEGG pathways, and Uniprot keywords. Enrichment was performed relative to all transcripts expressed in the mouse prefrontal cortex as defined by our RNA-seq data. Enriched functional terms were clustered at low stringency, to obtain clusters with enrichment score >1.2 (corresponding to an average p-value>0.05). See Table 2—source data 1 for details.

https://doi.org/10.7554/eLife.42819.018
Shank3ΔC vs. Wild Type
HomecageSleep Deprivation
Up-regulatedUp-regulated
Cholesterol Metabolism: Hmgcr, Insig1Potassium Ion Transport: Kcnv1, Kcnk1, Kcnk2
Transcription: Jun, Fosl2, Nfil3, Stat4Dephosphorylation: Dusp10, Dusp3, Ptprj
Neuron Projection Development: Cntn1, Ntrk2, Reln, Sema3a
Down-regulatedDown-regulated
MAPK Signaling: Mapk3 (ERK1), Elk1GnRH Signaling: Mapk1 (ERK2), Elk1, Mapk11 (p38)
Circadian Rhythms: Per3, Tef, HlfCircadian Rhythms: Per3, Nr1d1 (REV-ERBα), Tef, Hlf, Prkab2 (AMPK), Bhlhe41 (DEC2)
Transcription: Tef, HlfSodium Ion Transport: Slc6a15, Slc22a4, Slc24a4
Table 2—source data 1

Functional annotation clustering analysis of genes in Figure 4—source data 1.

https://doi.org/10.7554/eLife.42819.019

Wheel-running activity in Shank3ΔC mice is impaired in constant darkness

The results of our RNA-seq analysis revealed a general downregulation of circadian transcription factors in the mutants. These findings suggested that Shank3ΔC mice might also have abnormal circadian rhythms. To test this possibility, we measured wheel-running activity in Shank3ΔC and WT mice. Wheel-running data were collected for 2 weeks under LD (LD weeks 2–3), then mice were released into constant darkness (DD) for 3 weeks (DD weeks 1–3; Figure 5 and Figure 5—figure supplement 1). Alpha, the length of the active phase, was calculated as the time between activity onset and offset. Period length was the time elapsed from the start of an active phase to the start of the subsequent active phase. There was no differences between Shank3ΔC and WT mice for alpha or period length during the DD period (Table 3), but Shank3ΔC mice ran fewer revolutions than WT mice from LD week 3 – DD week 3 (F(1,338) = 30.96; p<0.0001; Table 3, Table 3—source data 1). Some Shank3ΔC mice greatly reduced their running at certain periods of the DD period, which is reflected in the variance for period, alpha, and activity measures (Figure 5, Figure 5—figure supplement 1, and Table 3). Over 5 weeks of continuous LD conditions, however, wheel-running activity for Shank3ΔC mice instead increased (F(1,542) = 85.99, p<0.0001; Supplementary file 1, Source data 1). Overall, these data indicate that constant darkness impairs wheel-running activity of Shank3ΔC mice.

Table 3
Shank3∆C mice show reduced wheel-running activity during constant darkness.

Summary of wheel-running behavior measured for each week of 12:12 hr light dark cycle (LD, 559 ± 4 : 0 ± 0 lux) or constant darkness (DD, 0 ± 0 lux) for both wild type and Shank3∆C mice. A linear mixed-effects model was used to estimate the contributions of genotype and the interaction between genotype and time for period, alpha, and wheel running activity for LD week three through DD week 3. There is a significant interaction effect for genotype x time for activity (p < 0.0001; indicated by *) but no significant effect for genotype x time for period (p = 0.15) or genotype x time for alpha (p = 0.88). See Table 3—source data 1 for details. Values are means ± SEM for wild type (n = 8) and Shank3∆C (n = 7) mice. Significance at p < 0.05.

https://doi.org/10.7554/eLife.42819.020
Wild TypeShank3∆C
Period (h)
LD Week 224.0 ± 0.024.0 ± 0.0
LD Week 324.0 ± 0.023.9 ± 0.1
DD Week 123.9 ± 0.123.7 ± 0.1
DD Week 223.7 ± 0.120.3 ± 3.4
DD Week 323.5 ± 0.225.8 ± 1.5
Alpha (h)
LD Week 210.0 ± 0.410.7 ± 0.4
LD Week 310.3 ± 0.410.7 ± 0.5
DD Week 110.5 ± 0.29.0 ± 0.4
DD Week 210.4 ± 0.48.2 ± 0.7
DD Week 39.0 ± 1.09.9 ± 1.0
Activity (rev/day)*
LD Week 233985.2 ± 565.923258.2 ± 861.2
LD Week 340306.7 ± 1257.125365.1 ± 964.0
DD Week 137942.6 ± 795.423027.7 ± 669.6
DD Week 238250.7 ± 431.116820.7 ± 1302.5
DD Week 338586.3 ± 231.16961.5 ± 383.9
Table 3—source data 1

Results of genotype x time interaction analysis for LD week three through DD week 3.

Linear mixed-effects ANOVA for LD week three through DD week 3. The model was fitted using genotype, time, and their interaction as fixed effects and the individual mice labeled as random effect. There is a significant effect of genotype x time for activity (p<0.0001) and no significant effect for genotype x time or genotype for period and alpha. Significance at p<0.05. numDF, numerator degrees of freedom. denDF, denominator degrees of freedom.

https://doi.org/10.7554/eLife.42819.021
Figure 5 with 1 supplement see all
Download asset Open asset
Shank3∆C mice show disruption of running wheel activity in constant darkness.

Representative actograms and periodograms for two wild type and two Shank3∆C mice. Mice were entrained to a 12:12 hr light:dark cycle (LD, 559 ± 4 : 0 ± 0 lux) for two weeks prior to 3 weeks constant darkness (DD, 0 ± 0 lux). Gray shading is representative of the dark period. (A) Actograms for two wild type mice. (B) Corresponding periodograms for wild type mice. (C) Actograms for two Shank3∆C mice. (D) Corresponding periodograms for Shank3∆C mice.

https://doi.org/10.7554/eLife.42819.022

Discussion

The present study is the first to establish a role for Shank3 in mammalian sleep. We show that both PMS patients and Shank3 mutant mice have trouble falling asleep (Figures 1, 2 and 3). This phenotype is widely observed in the ASD population and until now had not been replicated in animal models. Shank3∆C mice have problems falling asleep after periods of extended wakefulness when sleep pressure is high, such as at the end of the baseline dark period (Figure 2A) or following sleep deprivation (Figure 3D-E). They also have blunted NREM delta power (Figure 2B). However, Shank3∆C mice accumulate sleep pressure (Figure 3A) and show no gross abnormalities in circadian rhythms (Figure 5). This suggests that the primary deficit is in sleep onset.

Our molecular studies show that sleep deprivation increases differences in gene expression between Shank3∆C and WT mice (Figure 4). These differences point to the downregulation of circadian transcription factors and genes involved in the MAPK/GnRH pathways in the mutants (Table 2). Circadian transcription factors affected include Per3, Hlf, Tef, Nr1d1 (REV-ERBα), and Bhlhe41 (DEC2). We therefore investigated the effects of our Shank3 mutation in circadian rhythms. Shank3∆C mice do not show a disruption in circadian rhythmicity, but they do exhibit a large reduction in wheel-running activity in response to constant darkness (Figure 5, Table 3). Shank3∆C mice were reported to have deficits in motor coordination (Kouser et al., 2013); however, under a 12:12 hr LD schedule their wheel-running activity increases over time (Supplementary file 1, Source data 1). Daily rhythms of activity in rodents are linked to circadian oscillations in dopamine release (Feenstra et al., 2000; Menon et al., 2019) in the frontal cortex as well as in the striatum, a motor region with high levels of Shank3 expression (Peça et al., 2011). Together with a reduction in circadian gene expression, this DD-specific activity deficit suggests that the mutant sleep phenotype involves clock gene functions outside of their central time-keeping role. Interestingly, mutations in some of the circadian transcription factors we identified, Per3 and Bhlhe41 (DEC2), indeed lead to deficits in sleep regulation (Archer et al., 2018; He et al., 2009; Hirano et al., 2018). Our results support a role of clock genes in influencing sleep outside of their roles in generating circadian rhythms (Franken, 2013).

An interesting question is how can deletion of exon 21 of Shank3 lead to dysregulation of transcriptional and signaling pathways linked to sleep and sleep loss? Exon 21 of Shank3 encodes the homer and cortactin interaction domains of the protein. Homer interacts with metabotropic glutamate receptors (mGluRs) and SHANK3/homer complexes anchor mGluRs to the synapse. Shank3∆C mice show a marked reduction of the major isoforms of SHANK3 as well as an increase of mGluRs at the synapse (Kouser et al., 2013). mGluR signaling activates the MAPK/ERK pathway, a key regulator of activity-dependent transcription and synaptic plasticity in mature neurons (Thomas and Huganir, 2004). So the role of SHANK3 at the synaptic membrane explains the observed regulation of MAPK pathway genes (Table 2). However, it is not yet clear how SHANK3 regulates expression of circadian transcription factors within the nucleus. One possibility might include the role of SHANK3 in Wnt signaling. SHANK3 modulates Wnt-mediated transcriptional regulation by regulating internalization of Wnt receptor Frizzled-2 (Harris et al., 2016) and nuclear translocation of the Wnt ligand beta-catenin (Qin et al., 2018). The Wnt pathway kinase GSK3β phosphorylates circadian transcription factors PER2 (Iitaka et al., 2005), CRY2 (Harada et al., 2005), and REV-ERBα (Yin et al., 2006); however, this mechanism modulates circadian period length which is not altered in Shank3ΔC mice. A second potential mechanism is nuclear translocation of SHANK3 itself. SHANK3 is known to undergo synaptic-nuclear shuttling in response to neuronal activity and interact with nuclear ribonucleoproteins and components of the RNA Pol II mediator complex (Grabrucker et al., 2014). Deletion of the C-terminus leads to nuclear accumulation of SHANK3 and alterations in gene expression (Cochoy et al., 2015; Grabrucker et al., 2014). Thus, mutations in exon 21 of Shank3 could lead to deficits in transcriptional regulation in response to sleep deprivation through direct regulation of transcription in the nucleus. Yeast two-hybrid data show that SHANK3 can directly bind the circadian transcription factors REV-ERBα (encoded by Nr1d1) and DEC1 (encoded by Bhlhe40), a close paralog of DEC2 (Bhlhe41) (Sakai et al., 2011). Future studies of the effects of sleep on SHANK3 nuclear translocation will provide new insights into the non-synaptic function of shank proteins and their role in sleep and circadian rhythms.

Materials and methods

Key resources table
Reagent type
(species) or
resource		DesignationSource or
reference		Identifiers
Gene (Mus musculus)Shank3; SH3 and multiple ankyrin repeat domains 3NAENTREZ_ID: 58234
Gene (Mus musculus)Per3; period circadian clock 3NAENTREZ_ID: 18628
Gene (Mus musculus)Hlf; hepatic leukemia factorNAENTREZ_ID: 217082
Gene (Mus musculus)Tef; thyrotroph embryonic factorNAENTREZ_ID: 21685
Gene (Mus musculus)Nr1d1; nuclear receptor subfamily 1, group D, member 1;NAENTREZ_ID: 217166
Gene (Mus musculus)Bhlhe41; basic helix-loop-helix family, member e41;NAENTREZ_ID: 79362
Strain, strain background (Mus musculus)Shank3ΔC; Shank3tm1.1Pfw/JKouser et al., 2013; The Jackson LaboratoryRRID:IMSR_JAX:018398
Commercial assay or kitRNAeasy kitQiagen74104
Commercial assay or kitHigh Sensitivity RNA Analysis KitAdvanced Analytical TechnologiesDNF-472
Commercial assay or kitHigh Sensitivity NGS Fragment Analysis KitAdvanced Analytical TechnologiesDNF-474
Commercial assay or kitKAPA Library Quantification KitKapabiosystemsKK4824
Commercial assay or kitTruSeq Stranded mRNA Library Prep KitIlluminaRS-122–2101
Software, algorithmHiSeq Control Software version (v. 2.2.68)Illumina15073358 Rev A
Software, algorithmbcl2fastq (v. 2.17.1.14)R/BioconductorSCR_015058
Software, algorithmRsubread (v. 1.26.1)R/BioconductorSCR_016945
Software, algorithmNOISeq (v.2.22.1)R/BioconductorSCR_003002
Software, algorithmRUVSeq (v. 1.12.0)R/BioconductorSCR_006263
Software, algorithmedgeR (v. 3.20.9)R/BioconductorSCR_012802
Software, algorithmR package nlme (v. 3.5.0)CRANSCR_015655
Software, algorithmR package mgcf (v. 3.5.0)CRAN
Software, algorithmR package pheatmap (v. 1.0.10)CRANSCR_016418
Software, algorithmDatabase for annotation, visualization, and integrated discovery (v 6.7)DAVID; https://david.ncifcrf.gov
Software, algorithmVitalRecorderKissei Comtec3, 0, 0, 0
Software, algorithmSleepSign for AnimalKissei Comtec3, 0, 0, 812
Software, algorithmWheel managerMed Associates IncSOF-860
Software, algorithmClockLab (v. 3.5.0)Actimetrics6.0.50

Sleep questionnaire study

Request a detailed protocol

Sleep questionnaire data for PMS patients was obtained through a data access agreement with the PMSIR. The PMSIR contains demographic, clinical, and genetic data on PMS patients. Parents and caregivers are able to create a profile on behalf of the patient, enter demographic data, complete questionnaires on symptoms and development, and upload files such as genetic test reports and other medical records. Trained genetic counselors curate all genetic data to ensure each patient has as complete and accurate ‘genetic profile’ insofar as possible. Researchers may apply for data exports containing de-identified clinical data, developmental data, and genetic data. Once approved, the appropriate search is performed in the database and the results are provided to the researcher. Data in this study corresponds to a database export performed on 12/1/2016 containing the results of a sleep questionnaire completed by caregivers, as well as biographic and genetic information of PMS individuals. Difficulty falling asleep is defined as needing more than an hour to fall asleep. Multiple night awakenings is defined as more than two awakenings. Reduced sleep time is defined as less than 6 hr per night. Parasomnias are defined as abnormal movements, behaviors, emotions, perceptions, and dreams that occur while falling asleep or sleeping. Presence of sleep apnea is defined as having received a diagnosis of sleep apnea. Only individuals with a genetic counselor-confirmed deletion of the SHANK3 gene were included in the analysis. The final dataset included 176 individuals – 78 males and 98 females (age range 1–39 years).

Animals

Heterozygous Shank3+/∆C mice were obtained from Dr. Paul Worley at Johns Hopkins University. Shank3+/∆C breeding pairs were established to obtain wild type (WT) and Shank3∆C littermates. Mice were housed in standard cages at 24 ± 1°C on a 12:12 hr light:dark cycle (unless otherwise specified) with food and water ad libitum. All experimental procedures were approved by the Institutional Animal Care and Use Committee of Washington State University and conducted in accordance with National Research Council guidelines and regulations for experiments in live animals.

Assessment of sleep-wake behavior

Surgical procedures

Request a detailed protocol

Adult male mice (12-weeks-old) were stereotaxically implanted with electroencephalographic (EEG) and electromyographic (EMG) electrodes under isoflurane anesthesia according to previously published methods (Frank et al., 2002). Briefly, four stainless steel screws (BC-002MPU188, Bellcan International Corp, Hialeah, FL) were placed contralaterally over frontal (2) and parietal (2) cortices, and 2 EMG electrodes were inserted in the nuchal muscles. Mice were allowed 5 days of recovery from surgery prior to habituation to the recording environment.

Experimental design

Request a detailed protocol

Six days after surgery, wild type (WT; n = 10) and Shank3∆C (n = 10) littermates were connected to a lightweight, flexible tether and allowed 5 days to habituate to the tether and recording environment. After habituation, mice underwent 24 hr undisturbed baseline EEG and EMG recording starting at light onset (hour 1). The next day, mice were sleep deprived for 5 hr (hours 1–5) via gentle handling beginning at light onset according to previously published methods (Halassa et al., 2009; Vecsey et al., 2012; Ingiosi et al., 2015). Mice were then allowed 19 hr undisturbed recovery sleep (hours 6–12 of the light period and hours 13–24 of the dark period).

EEG/EMG data acquisition and analysis

Request a detailed protocol

EEG and EMG data were collected with Grass 7 polygraph hardware (Natus Medical Incorporated, Pleasanton, CA) via a light-weight, counterbalanced cable, amplified, and digitized at 200 Hz using VitalRecorder acquisition software (SleepSign for Animal, Kissei Comtec Co., LTD, Nagano, Japan). EEG and EMG data were high- and low-pass filtered at 0.3 and 100 Hz and 10 and 100 Hz, respectively.

Wakefulness and sleep states were determined by visual inspection of the EEG waveform, EMG activity, and fast Fourier transform (FFT) analysis by an experimenter blinded to the genotype. Data were scored as wakefulness, NREM sleep, or REM sleep with 4 s resolution using SleepSign for Animal as previously described (Halassa et al., 2009).

The EEG was subjected to fast Fourier transform analysis to produce power spectra between 0–20 Hz with 0.781 Hz resolution. Delta (δ) was defined as 0.5–4 Hz, theta (θ) as 5–9 Hz, and alpha (α) as 10–15 Hz. For genotypic comparisons of light and dark period spectral data, each spectral bin was expressed as a percentage of the sum of total state-specific EEG power (0–20 Hz) of the baseline 12 hr light period and 12 hr dark period, respectively (Baracchi and Opp, 2008). The relation between EEG spectral power and frequency under baseline conditions was fit to smooth curves and 95% confidence intervals for the respective light and dark periods. The analysis was performed via Generalized Additive Models (GAM) (Hastie and Tibshirani, 1990) using the R package mgcf (v. 3.5.0). For hourly NREM delta power analysis after sleep deprivation, spectral values within the delta band for each hour were normalized to the average NREM delta band value of the last 4 hr of the baseline light period (hours 9–12) and expressed as a percentage (Franken et al., 2001). Changes in individual NREM EEG spectral bins (0–20 Hz) after sleep deprivation were normalized to corresponding baseline spectral bins obtained from mean values from the last 4 hr of the baseline light period (hours 9–12). EEG epochs containing artifacts were excluded from spectral analyses.

Statistical analyses were performed using SPSS for Windows (IBM Corporation, Armonk, NY) and R statistical language. Data are presented as means ± standard error of the mean (SEM). If an individual hour bin did not contain a full hour of data due to technical issues (e.g. removal of recording tether), that hour was excluded from analysis for the corresponding individual animal. A general linear model for repeated measures (RM) using time (hours or period) as the repeated measure and genotype (WT vs. Shank3∆C) or treatment (undisturbed baseline vs. sleep deprivation) as the between subjects factor was used when multiple measurements were made over time (i.e. total sleep time, time in stage, bout number, bout duration, hourly NREM delta power). Repeated measures were tested for sphericity, and a Greenhouse-Geisser correction was applied when appropriate. Posthoc pairwise comparisons using Sidak corrections were performed when there were significant interaction effects or main effects of genotype or treatment. Baseline time in state and bout data RM comparisons were made over all time intervals during the full 24 hr recording period (hours 1–24). For sleep deprivation experiments, time in state and bout data RM comparisons were made over all time intervals during the full recovery period (hours 6–24). Hourly NREM delta power RM comparisons post-sleep deprivation were made over hours 6–12 of the recovery phase. Comparisons of normalized NREM EEG spectral power changes after sleep deprivation were made using one-way ANOVA with total spectral power as the dependent variable and genotype as the between subjects factor. If the one-way ANOVA yielded a significant result, unpaired Student’s t-tests with Benjamini-Hochberg correction were then used for comparisons of individual NREM EEG spectral frequency bins with genotype as the grouping variable. Unpaired Student’s t-tests with genotype as the grouping variable were used to analyze latency to state and total time in state post-sleep deprivation. Latency to NREM or REM sleep after sleep deprivation was defined as time elapsed from release to recovery sleep to the first bout of NREM sleep (bout ≥28 s or seven consecutive epochs) or REM sleep (bout ≥16 s or four consecutive epochs).

Genome-wide gene expression

RNA isolation

Request a detailed protocol

Adult male (8–10 week-old) Shank3∆C mice and WT littermates were divided into two groups: homecage controls (WT n = 5; Shank3∆Cn = 5) and sleep deprived (WT n = 5; Shank3∆Cn = 5). All mice were individually housed. Homecage control mice were left undisturbed and sacrificed 5 hr after light onset (hour 6). Mice in the sleep deprived group were sleep deprived for 5 hr via gentle handling starting at light onset (hour 1) and then sacrificed upon completion of sleep deprivation (hour 6) without allowing for any recovery sleep. Mice were sacrificed by live cervical dislocation (alternating between homecage controls and sleep deprived mice), decapitated, and prefrontal cortex was swiftly dissected on a cold block. Tissue was flash frozen in liquid nitrogen and stored at −80°C until processing (Poplawski et al., 2016; Tudor et al., 2016). To avoid day-specific effects on gene expression, this protocol was repeated over a 5 day period, and one sample from each genotype + treatment group was collected per day. Tissue was collected within the first 15 min of the hour. All tissue samples were later homogenized in Qiazol buffer (Qiagen, Hilden, Germany) using a TissueLyser (Qiagen) and all RNA was extracted using the Qiagen RNAeasy kit (Qiagen) on the same day.

RNA-seq library preparation and sequencing

Request a detailed protocol

The integrity of total RNA was assessed using Fragment Analyzer (Advanced Analytical Technologies, Inc, Ankeny, IA) with the High Sensitivity RNA Analysis Kit (Advanced Analytical Technologies, Inc). RNA Quality Numbers (RQNs) from 1 to 10 were assigned to each sample to indicate its integrity or quality. ‘10’ stands for a perfect RNA sample without any degradation, whereas ‘1’ marks a completely degraded sample. All RNA samples had RQNs ranging from 8.8 to 10 and were used for RNA library preparation with the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA). Briefly, mRNA was isolated from 2.5 µg of total RNA using poly-T oligo attached to magnetic beads and then subjected to fragmentation, followed by cDNA synthesis, dA-tailing, adaptor ligation, and PCR enrichment. The sizes of RNA libraries were assessed by Fragment Analyzer with the High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical Technologies, Inc). The concentrations of RNA libraries were measured by StepOnePlus Real-Time PCR System (ThermoFisher Scientific, San Jose, CA) with the KAPA Library Quantification Kit (Kapabiosystems, Wilmington, MA). The libraries were diluted to 2 nM with Tris buffer (10 mM Tris-HCl, pH 8.5) and denatured with 0.1 N NaOH. Eighteen pM libraries were clustered in a high-output flow cell using HiSeq Cluster Kit v4 on a cBot (Illumina). After cluster generation, the flow cell was loaded onto HiSeq 2500 for sequencing using HiSeq SBS kit v4 (Illumina). DNA was sequenced from both ends (paired-end) with a read length of 100 bp.

RNA-seq data analysis

Request a detailed protocol

HiSeq Control Software version 2.2.68 was used for base calling. The raw bcl files were converted to fastq files using the software program bcl2fastq2.17.1.14. Adaptors were trimmed from the fastq files during the conversion. The average sequencing depth for all samples was 52 million read pairs. Sequenced reads were mapped with GSNAP (parameter = -N 1 m 7 -A sam --nofails) to mm10. On average 84% of the sequenced reads mapped uniquely to the mm10 genome. All statistical analyses were performed using open source software freely available through the R/Bioconductor project (Gentleman et al., 2004). The count matrix was obtained using featureCounts (parameters = isPairedEnd = TRUE, requireBothEndsMapped = TRUE, annot.inbuilt = ‘mm10’) from the package Rsubread (v. 1.26.1) under Bioconductor (version 3.6) with R (v. 3.4.1) using mm10 built-in annotations (Mus_musculus.GRCm38.90) with ENTREZ ID as row names. Gene counts were filtered using a proportion test (counts per million cutoff of 1), as implemented in the NOISeq package (v.2.22.1) (Tarazona et al., 2015). Removal of unwanted variation (RUV) normalization was performed using RUVSeq (v. 1.12.0) (Risso et al., 2014; Peixoto et al., 2015) after the data was normalized by Trimmed Mean of M-values (TMM) (Robinson and Oshlack, 2010) using edgeR (v. 3.20.9) (Robinson et al., 2010). Specifically, RUVs (with k = 5) was used after defining groups based on both genotype and treatment and using a list of 2677 negative control genes obtained as genes with an adjusted p-value>0.9 in the comparison between sleep deprivation and controls in microarray data available through GEO (GSE78215) (obtained from Gerstner et al., 2016). Analogously, a list of 579 positive control genes obtained from the same study were used to evaluate the ability of our RNA-seq study to replicate known differences in gene expression. These positive controls were genes found to be differentially expressed (FDR < 0.005) by 5 hr of sleep deprivation relative to homecage controls in WT mice in four independent studies using two different microarray platforms and two different brain regions (Additional file 2 from Gerstner et al., 2016). Figure 4—figure supplement 1 shows that our RNA-seq study is able to recover 78% of the positive controls as differentially expressed when performing the same comparison done in the previous studies (HC5 vs SD5 in WT), while being able to detect many more differences in gene expression. This is a very high level of concordance considering these controls were obtained using a different technology and data were collected from experiments in four independent laboratories. Therefore, we are confident in the ability of our study to detect differences in gene expression that can be independently validated.

Differential expression analysis was performed using edgeR (v. 3.20.9) with a factorial design that included genotype (WT or Shank3∆C) and treatment (homecage control or sleep deprivation). We specified the following contrasts: wild type sleep deprived vs. wild type homecage controls (WTSD vs. WTHC), Shank3∆C sleep deprived vs. Shank3∆C homecage controls (S3SD vs. S3HC), Shank3∆C homecage controls vs. wild type homecage controls (S3HC vs. WTHC), and Shank3∆C sleep deprived vs. wild type sleep deprived (S3SD vs. WTSD). To gain insight on the different effects of sleep deprivation between wild type and mutant mice, we considered the union of the differentially expressed genes between genotypes both in homecage controls and sleep deprived mice. We clustered the genes using k-means (k = 3) and the samples using hierarchical clustering based on the selected genes. The resulting ordered genes and samples were visualized as a heatmap using the pheatmap package (v. 1.0.10). The R code used for RNA-seq data analysis is publicly available at https://github.com/drighelli/peixoto (Righelli et al., 2019; copy archived at https://github.com/elifesciences-publications/peixoto).

Functional annotation analysis of genes differentially expressed at FDR < 0.1 was based on Ensembl Gene IDs and performed using the database for annotation, visualization, and integrated discovery v 6.7 (DAVID, https://david.ncifcrf.gov). The following functional categories were used: GO Biological Process, GO Molecular Function, Uniprot keywords, and KEGG pathways. Enrichment was determined relative to all genes expressed in the mouse prefrontal cortex based on our data. We used an enrichment p-value (EASE) cutoff <0.1 for individual functional terms. Enriched functional terms were clustered at low stringency, to obtain clusters with an enrichment score >1.2 (corresponding to an average EASE >0.05).

Assessment of circadian rhythms

Experimental design

Request a detailed protocol

Adult male (9-week-old) wild type (n = 8) and Shank3∆C (n = 7) littermate mice were individually housed with a low-profile wireless running wheel (Med Associates Inc, Fairfax, VT) and kept under 12:12 hr light:dark (LD) cycle for 3 weeks (including 1 week of habituation) followed by 3 weeks of complete darkness (DD). Two Shank3∆C mice from a starting n of 9 were excluded from the experiments because they failed run on the wheel for the duration of the experiment. The experiment was repeated with an independent cohort of 11-week-old male wild type (n = 8) and Shank3∆C (n = 8) littermate mice kept under a 12:12 hr light:dark (LD) cycle for one week of habituation and then 5 weeks of data collection. One Shank3∆C mouse from a starting n of 9 was excluded from the second experiment because it failed to run on the wheel for the duration of the experiment. Daily checks were made to verify that each running wheel was transmitting data and that no light was detected in the room during constant darkness conditions.

Data acquisition and analysis

Request a detailed protocol

Wheel-running behavior was continuously monitored using Wheel Manager Software and exported into 10 min time bins (Med Associates Inc). ClockLab version 6.0.50 (Actimetrics, Wilmette, IL) was used for analysis. Period, the circadian length in time from activity onset to the next activity onset, was calculated using ClockLab’s chi-squared periodogram analysis function. Alpha, the length of the activity period, was calculated as the time from activity onset to offset (defined by manufacturer defaults). Activity was calculated as the total sum of wheel revolutions per day. A linear mixed-effects model ANOVA was used to estimate the contributions of genotype and the interaction between genotype and time for period, alpha, and wheel running activity for LD week three through DD week three in the first experiment, and LD week 1–5 in the second experiment. The model was fitted using genotype, time, and their interaction as fixed effects, and the individual mice were labeled as a random effect. The analysis was conducted with the R package nlme (v. 3.5.0) and is publicly available at https://github.com/drighelli/peixoto (Righelli et al., 2019; copy archived at https://github.com/elifesciences-publications/peixoto).

Data availability

Sequencing data have been deposited in GEO under accession code GSE113754. Source data files have been provided for Figures 1-4, Tables 1 and 2, and Supplementary Files 1 and 2. The R code used in this article is available on GitHub (https://github.com/drighelli/peixoto; copy archived at https://github.com/elifesciences-publications/peixoto). The R code used for the statistical analysis of RNA-seq and circadian wheel running data is also available in Source Code file 1.

The following data sets were generated
    1. Peixoto L
    2. Roser L
    3. Schoch H
    4. Wintler T
    (2018) NCBI Gene Expression Omnibus
    ID GSE113754. RNA-Seq analysis of Sleep deprivation in wildtype and Shank3 mutant mice.
    https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE113754
The following previously published data sets were used
    1. Peixoto L
    2. Frank M
    3. Gerstner J
    (2016) NCBI Gene Expression Omnibus
    ID GSE78215. Gene expression linked to sleep homeostasis in murine cortex.
    https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE78215

References

  1. Book
    1. Achermann P
    2. Borbely AA
    (2017) Sleep homeostasis and models of sleep regulation
    In: Kryger M, Roth T, Dement W. C, editors. Principles and Practice of Sleep Medicine (6th Ed). Philadelphia: Elsevier. pp. 377–387.
    https://doi.org/10.1016/b978-0-323-24288-2.00036-2
  2. Book
    1. American Psychiatric Association
    (2013)
    Diagnostic and Statistical Manual of Mental Disorders (5th Edition)
    Arlington, VA: American Psychiatric Association.
    1. Baweja R
    2. Calhoun S
    3. Baweja R
    4. Singareddy R
    (2013)
    Sleep problems in children
    Minerva Pediatrica 65:457–472.
  35. Book
    1. Hastie TJ
    2. Tibshirani RJ
    (1990)
    Generalized Additive Models (Monographs on Statistics and Applied Probability 43
    Chapman and Hall/CRC.
    1. Lozoff B
    2. Wolf AW
    3. Davis NS
    (1985)
    Sleep problems seen in pediatric practice
    Pediatrics 75:477–483.

Decision letter

  1. Huda Y Zoghbi
    Senior Editor; Texas Children's Hospital, United States
  2. Eunjoon Kim
    Reviewing Editor; Institute for Basic Science, Korea Advanced Institute of Science and Technology, Republic of Korea
  3. Valerie Mongrain
    Reviewer; Université de Montréal, Canada

In the interests of transparency, eLife includes the editorial decision letter and accompanying author responses. A lightly edited version of the letter sent to the authors after peer review is shown, indicating the most substantive concerns; minor comments are not usually included.

Thank you for submitting your article "Shank3 Modulates Sleep and Expression of Circadian Transcription Factors" for consideration by eLife. Your article has been reviewed by three peer reviewers, and the evaluation has been overseen by a Reviewing Editor and Huda Zoghbi as the Senior Editor. The following individual involved in the review of your submission has agreed to reveal her identity: Valerie Mongrain (Reviewer #3).

The reviewers have discussed the reviews with one another and the Reviewing Editor has drafted this decision to help you prepare a revised submission.

Summary:

The manuscript by Ingiosi et al., have reported the sleep problems in mice lacking exon 21 of Shank3, similar to PMS patients. Moreover, the RNAseq data have shown the sleep deprivation-induced increase of differential gene expression between Shank3ΔC and wild-types, including the downregulation of circadian transcription factors. Reduced wheel-running activity in constant darkness is also reported for Shank3ΔC mice. The results are potentially interesting and important. To strengthen the main conclusions of this paper, there are a few concerns that need to be addressed.

Essential revisions:

1) It is important to verify the RNAseq-identified changes in circadian transcription factors (Per3, Dec2, Hlf, Tef, Reverbα) in Shank3ΔC mice with qPCR experiments. Since "Most of the differences in gene expression following SD are not present in HC conditions", do these circadian transcription factors show differences between WT vs. Shank3ΔC only following SD?

2) To better understand the potential mechanisms underlying transcriptional dysregulation by deletion of exon 21 of Shank3, the authors need to provide evidence showing that the C-term truncated Shank3 (Shank3ΔC) accumulates in the nucleus of neurons from Shank3ΔC mice. If no such nuclear accumulation of Shank3ΔC can be found, an alternative mechanism is the nuclear translocation of some Shank3-interacting protein, such as β-catenin, in Shank3ΔC mice (Qin L, et al., 2018), which leads to transcriptional changes. This possibility needs to be incorporated.

3) Shank3ΔC mice have been reported to have impaired motor coordination (Kouser et al., 2013, but not Duffney et al., 2015). Could the wheel-running activity reduction of Shank3ΔC mice in constant darkness (DD) result from motor-coordination deficits? Is there any reduction of wheel-running activity during 5-week regular light-dark cycles (LD) for Shank3ΔC mice? What is the connection between sleep difficulties in Shank3ΔC mice and reduced wheel-running activity in DD?

4) Results suffer from the absence of main statistics in support of the data (e.g., F and p values are absent for describing the genotype differences in the time course of wakefulness and NREM sleep presented in Figure 2A and Figure 3—figure supplement 1; the manner by which data in Figure 2—source data 1 were compared is unclear, and F or t values should be reported; F values and degrees of freedom also missing for Table 2). Moreover, it is very unclear how the analyses of repeated measures were performed at multiple instances. For example, an adequate analysis of the time course of Figure 2A would be to use two genotypes by 24 hours ANOVAs, but it seems from the description of the results that different parts of the 24-hour period were analyzed in separated ANOVAs (similar comment applies to Figure 3—figure supplement 1). This requires clarification and correction if necessary. Also, the use of paired t-tests for comparing genotypes in variables related to wheel-running activity as indicated in the methods is inadequate.

[Editors' note: further revisions were requested prior to acceptance, as described below.]

Thank you for resubmitting your work entitled "Shank3 Modulates Sleep and Expression of Circadian Transcription Factors" for further consideration at eLife. Your revised article has been favorably evaluated by Huda Zoghbi (Senior Editor), a Reviewing Editor, and two reviewers.

The manuscript has been improved but there are some remaining issues that need to be addressed before acceptance, as outlined below:

Although the revised manuscript has addressed many of the review comments, reviewer #3 pointed out statistical issues that remain to be further addressed. We feel that it is important for these points to be fully addressed.

Reviewer #3:

This revised manuscript presenting the sleep phenotype, responses to sleep loss and locomotor activity rhythm in a Shank3 mouse model of neurodevelopmental disease has been modified by authors in aiming to address the previous comments of the reviewers and editor. As indicated before, the presented work brings novelty with regards to molecular determinants of sleep disturbances in psychiatric diseases, and has value in its multi-methodology approach. However, authors seem to have only partly responded to previous concerns and the improvement of the manuscript in comparison to its original version is thus modest (e.g., no qPCR validation using the same RNA material as the one used for RNA-seq, no data regarding the nuclear location of the truncated Shank3, unclear description and inadequate use of statistics).

I will solely highlight below the points following up on my previous comments for which I feel that adequate modifications were not implemented.

1) Repeated-measure ANOVAs for time courses of vigilance states have been repeated multiple times for the same datasets (i.e., 1 – 24 hours, then again for 1 – 12 hours and 13 – 24 hours, then a third time for 1 – 6 hours, 7 – 12 hours, 13 – 18 hours, 19 – 24 hours). Such design is inadequate because applying numerous (unnecessary) statistical tests increases the chance of getting false positives (i.e., increase Type I error rate). The authors need to perform only one repeated-measure ANOVA per vigilance state (per full 24-hour time course) and to apply post hoc comparisons of individual hours afterwards. Post hoc comparisons of individual hours will also make sure the authors are not picturing significant differences for hours without differences such as what can be seen at the moment for hour 24 in Figure 2A for instance (see also stars showing significant differences covering hours with very similar means in Figure 3—figure supplement 1).

2) Moreover, repeated-measure ANOVAs have not been corrected for repeated measures using an appropriate correction method (e.g., Greenhouse-Geisser or Huynh-Feldt corrections). This needs to be done at all instances for which repeated-measure ANOVAs were used (e.g., Figure 2, Figure 2—figure supplement 1).

3) Some analyses are performed inadequately with regards to the design such as the number of bouts and duration of bouts of Figure 2—figure supplement 1 and of Figure 3—figure supplement 2 for which t-tests were used for each interval instead of appropriate application of a repeated-measure ANOVA that would test all intervals simultaneously. This should be corrected.

4) Similarly, data of EEG spectral bands presented under Supplementary file 1 are inappropriately analysed with multiple independent t-tests. This should be modified for a more adequate (and combined) statistical test (e.g., Genotype [WT vs. Shank3deltaC] by Period [light vs. dark] by Condition [baseline vs. sleep deprivation] ANOVAs).

5) Finally, with regards to statistics, the fact that statistics are only presented in the Source data files is extremely inconvenient and is making findings of the main manuscript very unclear. The full statistics should be presented in figure legends or in the main text of the manuscript. Moreover, the main manuscript should also clearly indicate the design of the statistical tests that were used for every variable without referring to other publications.

6) In the revised manuscript, the authors defined "hour 1" as the first hour of the light period and "hour 13" as the first hour of the dark period. Nevertheless, this is not described in the manuscript, and is thus even more confusing than the previous version of the manuscript in which Zeitgeber time (which is well established) was not correctly used.

https://doi.org/10.7554/eLife.42819.033

Author response

Essential revisions:

1) It is important to verify the RNAseq-identified changes in circadian transcription factors (Per3, Dec2, Hlf, Tef, Reverbα) in Shank3ΔC mice with qPCR experiments. Since "Most of the differences in gene expression following SD are not present in HC conditions", do these circadian transcription factors show differences between WT vs. Shank3ΔC only following SD?

In consultation and agreement with the editors, it was decided that qPCR validation of the RNAseq results is outside of the scope of the current manuscript. This would require repeating a large part of the original study to obtain fresh samples. If the concerns are based on whether our RNA-seq results are reproducible, we addressed this issue by evaluating the ability of our study to reproduce findings from previous studies of genome-wide gene expression following sleep deprivation in mice as detailed in the methods section. Briefly, we assembled a list of 579 positive control genes found to be differentially expressed (FDR <0.005) by 5 hours of sleep deprivation relative to home cage controls in WT mice in 4 independent studies using two different microarray platforms and two different brain regions (Additional file 2, Gerstner et al., 2016). We then evaluated the ability of our RNA-seq study to recover the positive controls as differentially expressed when performing the same comparison done in the previous studies (HC5 vs SD5 in WT). Our RNA-seq study was able to recover 78% of the positive controls while being able to detect many more differences in gene expression. This is a very high level of concordance considering these controls were obtained using a different technology and data was collected from experiments in 4 independent laboratories. Therefore, we are confident in the ability of our study to detect differences in gene expression that can be independently validated. The Materials and methods section has been updated to make the reproducibility evaluation more clear and Figure 4—figure supplement 1 was added to show the results of this evaluation.

In regards to whether the differences in gene expression of circadian transcription factors is present in HC conditions, as shown in Table 1 three of the five circadian transcripts differentially expressed following SD are also downregulated in baseline HC conditions. In HC, Per3 (FC -0.27; FDR 0.008), Hlf (FC -0.14; FDR 0.03) and Tef (FC -0.19; FDR 0.02) are downregulated in Shank3ΔC mice. Nr1d1 (Reverbα) and Bhlhe41(Dec2) are not differentially expressed in HC. Genes differentially expressed in the mutants under HC and SD, as well as functional annotation of those genes, are reported in Figure 4, Figure 4—source data 1, and summarized in Table 1.

2) To better understand the potential mechanisms underlying transcriptional dysregulation by deletion of exon 21 of Shank3, the authors need to provide evidence showing that the C-term truncated Shank3 (Shank3-ΔC) accumulates in the nucleus of neurons from Shank3ΔC mice. If no such nuclear accumulation of Shank3-ΔC can be found, an alternative mechanism is the nuclear translocation of some Shank3-interacting protein, such as β-catenin, in Shank3ΔC mice (Qin L, et al., 2018), which leads to transcriptional changes. This possibility needs to be incorporated.

We thank the reviewer for suggesting this possible alternative mechanism. We amended the discussion to include Wnt signaling as an additional potential transcriptional regulatory mechanism as follows: “SHANK3 also modulates Wnt-mediated transcriptional regulation by regulating internalization of Wnt receptor Frizzled-2 (Harris et al., 2016) and nuclear translocation of the Wnt ligand β-catenin (Qin et al., 2018). The Wnt pathway kinase GSK3β phosphorylates circadian transcription factors PER2 (Iitaka et al., 2005), CRY2 (Harada et al., 2005) and REVERBα (Yin et al., 2006), however this mechanism modulates circadian period length which is not altered in Shank3ΔC mice.”

3) Shank3ΔC mice have been reported to have impaired motor coordination (Kouser et al., 2013, but not Duffney et al., 2015). Could the wheel-running activity reduction of Shank3ΔC mice in constant darkness (DD) result from motor-coordination deficits? Is there any reduction of wheel-running activity during 5-week regular light-dark cycles (LD) for Shank3ΔC mice? What is the connection between sleep difficulties in Shank3ΔC mice and reduced wheel-running activity in DD?

The reviewer is correct that impaired motor coordination has been reported in Shank3ΔC mice. To address the possibility that activity levels in Shank3ΔC mice change across extended periods of wheel running due to motor coordination problems, we submitted a new cohort of animals to 5-weeks of voluntary wheel-running under regular 12:12 LD cycles. The results are shown in Supplementary file 2 and Source data 1. Under constant LD conditions, activity levels in Shank3ΔC mice do not decrease over time. Therefore, we conclude that the reduction in wheel running we observed in constant darkness (DD) conditions is not due to motor problems.

As to the connection between sleep problems and reduced DD wheel-running activity, this is a very interesting question that we currently do not have enough data to address. In the future, simultaneous recordings of sleep and circadian activity will help us better answer this question and we thank the reviewer for the suggestion.

4) Results suffer from the absence of main statistics in support of the data (e.g., F and p values are absent for describing the genotype differences in the time course of wakefulness and NREM sleep presented in Figure 2A and Figure 3—figure supplement 1; the manner by which data in Figure 2—source data 1 were compared is unclear, and F or t values should be reported; F values and degrees of freedom also missing for Table 2). Moreover, it is very unclear how the analyses of repeated measures were performed at multiple instances. For example, an adequate analysis of the time course of Figure 2A would be to use two genotypes by 24 hours ANOVAs, but it seems from the description of the results that different parts of the 24-hour period were analyzed in separated ANOVAs (similar comment applies to Figure 3—figure supplement 1). This requires clarification and correction if necessary. Also, the use of paired t-tests for comparing genotypes in variables related to wheel-running activity as indicated in the methods is inadequate.

To better support the statistical analysis of our data, we added additional information to support the statistics for mouse EEG data as suggested by the reviewer, including F and t-values and degrees of freedom (Figure 2—source data 2 and Figure 3—source data 2)

Repeated measures ANOVAs on sleep data in Figure 2 and Figure 3 were performed as previously described (Ingiosi et al., 2015; Ingiosi and Opp, 2016). We have added those references to the methods section. It is not uncommon when performing analysis of sleep data in mice to consider separately periods of the day in which the amount and quality of sleep are known to be different (e.g. light versus dark phase). Post-SD data is usually analyzed considering the rest of the light phase following SD, and then the subsequent dark-phase. We added additional comparisons across longer time periods as suggested by the reviewer (Figure 2—source data 2 and Figure 3—source data 2).

Last, we agree with the reviewer and have removed the individual t-tests from the wheel-running analysis. ANOVA statistics for LD:DD and LD:LD wheel running experiments can be found in Table 2—source data 1 and Supplementary file 2—source data 1.

[Editors' note: further revisions were requested prior to acceptance, as described below.]

Reviewer #3:

[…] 1) Repeated-measure ANOVAs for time courses of vigilance states have been repeated multiple times for the same datasets (i.e., 1 – 24 hours, then again for 1 – 12 hours and 13 – 24 hours, then a third time for 1 – 6 hours, 7 – 12 hours, 13 – 18 hours, 19 – 24 hours). Such design is inadequate because applying numerous (unnecessary) statistical tests increases the chance of getting false positives (i.e., increase Type I error rate). The authors need to perform only one repeated-measure ANOVA per vigilance state (per full 24-hour time course) and to apply post hoc comparisons of individual hours afterwards. Post hoc comparisons of individual hours will also make sure the authors are not picturing significant differences for hours without differences such as what can be seen at the moment for hour 24 in Figure 2A for instance (see also stars showing significant differences covering hours with very similar means in Figure 3—figure supplement 1).

We included a repeated measures ANOVA on baseline data across the full 24 h per vigilance state in our first revision. We now also include repeated measures ANOVAs across the 19 hour recovery phase after sleep deprivation for each vigilance state. We removed the 12 hour- and 6 hour-binned repeated measures ANOVAs as suggested and now report posthoc pairwise comparisons with Sidak correction for individual hours when there are main effects of genotype or significant interactions between genotype and time. To provide a simpler presentation of overall light-dark differences in sleep time, we now include a new Table 1 that reports total sleep time in light and dark periods under baseline conditions.

2) Moreover, repeated-measure ANOVAs have not been corrected for repeated measures using an appropriate correction method (e.g., Greenhouse-Geisser or Huynh-Feldt corrections). This needs to be done at all instances for which repeated-measure ANOVAs were used (e.g., Figure 2, Figure 2—figure supplement 1).

All repeated measures ANOVAs were tested for sphericity, and a Greenhouse-Geisser correction was applied when appropriate. We updated the Materials and methods section to include this detail.

3) Some analyses are performed inadequately with regards to the design such as the number of bouts and duration of bouts of Figure 2—figure supplement 1 and of Figure 3—figure supplement 2 for which t-tests were used for each interval instead of appropriate application of a repeated-measure ANOVA that would test all intervals simultaneously. This should be corrected.

The reviewer is correct that statistical comparisons in Figure 2—figure supplement 1 and Figure 3—figure supplement 2 could be improved. To better support the statistical analysis of our data, we conducted repeated measures ANOVAs across all time intervals simultaneously for number of bouts and duration of bouts for each vigilance state. Posthoc pairwise comparisons with Sidak correction were conducted when permissible.

4) Similarly, data of EEG spectral bands presented under Supplementary file 1 are inappropriately analysed with multiple independent t-tests. This should be modified for a more adequate (and combined) statistical test (e.g., Genotype [WT vs. Shank3deltaC] by Period [light vs. dark] by Condition [baseline vs. sleep deprivation] ANOVAs).

The table in Supplementary file 1 was confusing as presented, added little to the study, and is now removed for clarity. Our principle EEG findings are that Shank3ΔC mice have altered EEGs, accumulate NREM delta power after sleep deprivation, and show an abnormal homeostatic enhancement of higher frequencies after sleep deprivation. This information can be found in Figure 2B, Figure 3A, and Figure 3B–C, respectively. Removal of the Supplementary file 1 table simplifies the presentation of this information.

5) Finally, with regards to statistics, the fact that statistics are only presented in the Source data files is extremely inconvenient and is making findings of the main manuscript very unclear. The full statistics should be presented in figure legends or in the main text of the manuscript. Moreover, the main manuscript should also clearly indicate the design of the statistical tests that were used for every variable without referring to other publications.

We now report F and t statistics and p-values within the main text where specific findings are stated. We also added the statistical tests used in the figure legends. Due to the large number of statistical results that we are reporting, we followed the guidelines provided by the journal with respect to source data files:

“Authors should provide information about data processing and analysis in their figure legends, including any statistical tests applied, with exact sample number, p values of tests, criteria for data inclusion or exclusion, and details of replicates. In some cases, it might be unwieldy to have this information in the legend of a figure, in which case the information can be provided in a source data file.”

Additionally, we expanded the Materials and methods section to provide a clearer and more detailed description of the design of our statistical tests.

6) In the revised manuscript, the authors defined "hour 1" as the first hour of the light period and "hour 13" as the first hour of the dark period. Nevertheless, this is not described in the manuscript, and is thus even more confusing than the previous version of the manuscript in which Zeitgeber time (which is well established) was not correctly used.

All data are now presented as hours 1 – 24 which is also used in the sleep field and accessible to others outside of sleep and circadian research (who may be unfamiliar with the term ‘Zeitgeber’). We further clarified “hour 1” as light onset and “hour 13” as dark onset in the Materials and methods section. Changing to Zeitgeber time would require substantial edits to the text, figures, and source files, and this change would not impact the outcome of the experiments.

https://doi.org/10.7554/eLife.42819.034

Article and author information

Author details

  1. Ashley M Ingiosi

    Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University, Spokane, United States
    Contribution
    Conceptualization, Formal analysis, Investigation, Visualization, Methodology, Writing—original draft, Writing—review and editing
    Contributed equally with
    Hannah Schoch
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-3035-3010

  2. Hannah Schoch

    Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University, Spokane, United States
    Contribution
    Data curation, Formal analysis, Investigation, Visualization, Writing—review and editing
    Contributed equally with
    Ashley M Ingiosi
    Competing interests
    No competing interests declared

  3. Taylor Wintler

    Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University, Spokane, United States
    Contribution
    Formal analysis, Investigation, Visualization, Methodology, Writing—original draft, Writing—review and editing
    Competing interests
    No competing interests declared

  4. Kristan G Singletary

    Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University, Spokane, United States
    Contribution
    Investigation, Project administration, Writing—review and editing
    Competing interests
    No competing interests declared

  5. Dario Righelli

    1. Istituto per le Applicazioni del Calcolo “M. Picone”, Consiglio Nazionale della Ricerche, Napoli, Italy
    2. Dipartimento di Scienze Aziendali Management & Innovation Systems, University of Fusciano, Fisciano, Italy
    Contribution
    Formal analysis, Visualization, Methodology, Writing—review and editing
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-1504-3583

  6. Leandro G Roser

    Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University, Spokane, United States
    Contribution
    Data curation, Formal analysis, Methodology, Writing—review and editing
    Competing interests
    No competing interests declared

  7. Elizabeth Medina

    Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University, Spokane, United States
    Contribution
    Investigation, Methodology
    Competing interests
    No competing interests declared

  8. Davide Risso

    1. Department of Statistical Sciences, University of Padova, Padova, Italy
    2. Division of Biostatistics and Epidemiology, Department of Healthcare Policy and Research, Weill Cornell Medicine, New York, United States
    Contribution
    Conceptualization, Supervision, Visualization, Methodology, Writing—review and editing
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-8508-5012

  9. Marcos G Frank

    Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University, Spokane, United States
    Contribution
    Conceptualization, Resources, Supervision, Methodology, Writing—review and editing
    For correspondence
    marcos.frank@wsu.edu
    Competing interests
    No competing interests declared

  10. Lucia Peixoto

    Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University, Spokane, United States
    Contribution
    Conceptualization, Resources, Data curation, Formal analysis, Supervision, Funding acquisition, Investigation, Visualization, Methodology, Writing—original draft, Project administration, Writing—review and editing
    For correspondence
    lucia.peixoto@wsu.edu
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-8444-9600

Funding

National Institute of Neurological Disorders and Stroke (K01NS104172)

  • Lucia Peixoto

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Acknowledgements

Data used in the preparation of this article were obtained from the Phelan-McDermid Syndrome International Registry (PMSIR) version dated 12/1/2016.

We thank Dr. Paul Worley for providing the Shank3 mutant mouse line. We thank Dr. Hans Van Dongen and Dr. John Hogenesch for valuable discussion of the results. We thank Dr. Claudia Angelini for valuable discussion of the statistical methods.

Ethics

Human subjects: PMSIR patient data was obtained de-identified under IRB exemption 15005-Peixoto

Animal experimentation: This study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All of the animals were handled according to approved institutional animal care and use committee (IACUC) protocols of Washington State University. Protocol numbers: 04705-001 and 04704-001 IACUC 6155-Peixoto Breeding IACUC 4705-Peixoto experimental IACUC 4581-Frank experimental

Senior Editor

  1. Huda Y Zoghbi, Texas Children's Hospital, United States

Reviewing Editor

  1. Eunjoon Kim, Institute for Basic Science, Korea Advanced Institute of Science and Technology, Republic of Korea

Reviewer

  1. Valerie Mongrain, Université de Montréal, Canada

Version history

  1. Received: October 14, 2018
  2. Accepted: April 10, 2019
  3. Accepted Manuscript published: April 11, 2019 (version 1)
  4. Version of Record published: April 29, 2019 (version 2)

Copyright

© 2019, Ingiosi et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 5,433
    Page views
  • 792
    Downloads
  • 47
    Citations

Article citation count generated by polling the highest count across the following sources: Crossref, Scopus, PubMed Central.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Open citations (links to open the citations from this article in various online reference manager services)

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

  1. Ashley M Ingiosi
  2. Hannah Schoch
  3. Taylor Wintler
  4. Kristan G Singletary
  5. Dario Righelli
  6. Leandro G Roser
  7. Elizabeth Medina
  8. Davide Risso
  9. Marcos G Frank
  10. Lucia Peixoto
(2019)
Shank3 modulates sleep and expression of circadian transcription factors
eLife 8:e42819.
https://doi.org/10.7554/eLife.42819

Categories and tags

Research organism

Further reading

    1. Genetics and Genomics
    2. Neuroscience

    Translation of dipeptide repeat proteins in C9ORF72 ALS/FTD through unique and redundant AUG initiation codons

    Yoshifumi Sonobe, Soojin Lee ... Paschalis Kratsios
    Research Article Updated

    A hexanucleotide repeat expansion in C9ORF72 is the most common genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). A hallmark of ALS/FTD pathology is the presence of dipeptide repeat (DPR) proteins, produced from both sense GGGGCC (poly-GA, poly-GP, poly-GR) and antisense CCCCGG (poly-PR, poly-PG, poly-PA) transcripts. Translation of sense DPRs, such as poly-GA and poly-GR, depends on non-canonical (non-AUG) initiation codons. Here, we provide evidence for canonical AUG-dependent translation of two antisense DPRs, poly-PR and poly-PG. A single AUG is required for synthesis of poly-PR, one of the most toxic DPRs. Unexpectedly, we found redundancy between three AUG codons necessary for poly-PG translation. Further, the eukaryotic translation initiation factor 2D (EIF2D), which was previously implicated in sense DPR synthesis, is not required for AUG-dependent poly-PR or poly-PG translation, suggesting that distinct translation initiation factors control DPR synthesis from sense and antisense transcripts. Our findings on DPR synthesis from the C9ORF72 locus may be broadly applicable to many other nucleotide repeat expansion disorders.

    1. Cell Biology
    2. Neuroscience

    Removal of extracellular human amyloid beta aggregates by extracellular proteases in C. elegans

    Elisabeth Jongsma, Anita Goyala ... Collin Yvès Ewald
    Research Article Updated

    The amyloid beta (Aβ) plaques found in Alzheimer’s disease (AD) patients’ brains contain collagens and are embedded extracellularly. Several collagens have been proposed to influence Aβ aggregate formation, yet their role in clearance is unknown. To investigate the potential role of collagens in forming and clearance of extracellular aggregates in vivo, we created a transgenic Caenorhabditis elegans strain that expresses and secretes human Aβ1-42. This secreted Aβ forms aggregates in two distinct places within the extracellular matrix. In a screen for extracellular human Aβ aggregation regulators, we identified different collagens to ameliorate or potentiate Aβ aggregation. We show that a disintegrin and metalloprotease a disintegrin and metalloprotease 2 (ADM-2), an ortholog of ADAM9, reduces the load of extracellular Aβ aggregates. ADM-2 is required and sufficient to remove the extracellular Aβ aggregates. Thus, we provide in vivo evidence of collagens essential for aggregate formation and metalloprotease participating in extracellular Aβ aggregate removal.

    1. Computational and Systems Biology
    2. Neuroscience

    Task-dependent optimal representations for cerebellar learning

    Marjorie Xie, Samuel P Muscinelli ... Ashok Litwin-Kumar
    Research Article Updated

    The cerebellar granule cell layer has inspired numerous theoretical models of neural representations that support learned behaviors, beginning with the work of Marr and Albus. In these models, granule cells form a sparse, combinatorial encoding of diverse sensorimotor inputs. Such sparse representations are optimal for learning to discriminate random stimuli. However, recent observations of dense, low-dimensional activity across granule cells have called into question the role of sparse coding in these neurons. Here, we generalize theories of cerebellar learning to determine the optimal granule cell representation for tasks beyond random stimulus discrimination, including continuous input-output transformations as required for smooth motor control. We show that for such tasks, the optimal granule cell representation is substantially denser than predicted by classical theories. Our results provide a general theory of learning in cerebellum-like systems and suggest that optimal cerebellar representations are task-dependent.