Guy1, a Y-linked embryonic signal, regulates dosage compensation in Anopheles stephensi by increasing X gene expression
- Virginia Tech, United States
- Southern Medical University, China
- Virginia Biocomplexity Institute, United States
Abstract
We previously showed that Guy1, a primary signal expressed from the Y chromosome, is a strong candidate for a male-determining factor that confers female-specific lethality in Anopheles stephensi (Criscione et al., 2016). Here we present evidence that Guy1 increases X gene expression in Guy1-transgenic females from two independent lines, providing a mechanism underlying the Guy1-conferred female lethality. The median level gene expression (MGE) of X-linked genes is significantly higher than autosomal genes in Guy1-transgenic females while there is no significant difference in MGE between X and autosomal genes in wild type females. Furthermore, Guy1 significantly up-regulates at least 40% of the 996 genes across the X chromosome in transgenic females. Guy1-conferred female-specific lethality is remarkably stable and completely penetrant. These findings indicate that Guy1 regulates dosage compensation in An. stephensi and components of dosage compensation may be explored to develop novel strategies to control mosquito-borne diseases.
Data availability
Data submitted to SRA, PRJNA503140 : The transcriptome of Guy1-transgenic Anopheles stephensi L1 instar (TaxID: 30069)
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The transcriptome of Guy1-transgenic Anopheles stephensi L1 instarNCBI Bioproject, PRJNA503140.
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Author details
Funding
National Institutes of Health (AI105575)
- Zhijian Jake Tu
National Institutes of Health (AI121284)
- Zhijian Jake Tu
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: This study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All of the animals were handled according to approved institutional animal care and use committee (IACUC) protocols (IACUC #16-067) of the Virginia Tech.
Copyright
© 2019, Qi et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Guy1, a Y-linked embryonic signal, regulates dosage compensation in Anopheles stephensi by increasing X gene expression
eLife 8:e43570.
https://doi.org/10.7554/eLife.43570
