(A) Growth of the indicated anchor-away cells (5-fold serial dilutions) in the presence or absence of rapamycin. (B) Occupancy of the indicated FRB-tagged GTFs at the PMA1 and CCW12 promoters in the …
Pol II occupancy at the PMA1 and RPL28 coding regions in the indicated strains grown in the presence or absence of rapamycin.
(A) Mean Pol II occupancy averaged over 453 well-transcribed genes (metagene analysis) in strains depleted (+rap) for the indicated factor and in the parental (WT) strain (±rap). Partial reduction …
(A) Mean Pol II occupancy at the HSP12 coding region (ORF) and promoter in strains depleted (or not) for the indicated factor and then induced for 15 min by shifting to 39°C. (B) FRB-tagged GTF:Pol …
(A) Pol II occupancy at the coding region (ORF) and promoter of the indicated genes in strains depleted (or not) for the indicated factor and then induced for 15 min by shifting to 39°C. (B) …
(A) TBP occupancy at the CCW12 and TEF1 promoters in strains depleted (+rap) or not (-rap) for the indicated factor. (B) TBP occupancy at the HSP12 and HSP82 promoters in strains depleted (+rap) or …
(A) TBP occupancy at the PMA1 and ACT1 promoters in strains depleted (+rap) or not (-rap) for the indicated factor. (B) TBP occupancy at the SSA4 and SED1 promoters in strains depleted (+rap) or not …
(A) Pol II (ORF and promoter) and TBP occupancies at promoters (PMA1 and RPL28) of genes inhibited by metabolic stress in cells depleted for uracil or leucine for various times (color scale). (B) …
(A) TBP occupancy at Pol III (tRNA) promoters, as well as growth curves for cultures depleted of uracil or leucine. (B) Pol II (ORF and promoter) and TBP occupancies at promoters of genes inhibited …
Mean Pol II occupancy and TFIIB occupancy averaged over 453 well-transcribed genes and promoters before and after Spt16 depletion.
(A) Mean Pol II occupancy averaged over 453 well-transcribed ‘SAGA’ or ‘TFIID’ genes in parental or Taf1-depleted strains. (B) Pol II (C) TBP, TFIIB, and TFIIA, and (D) Taf1 occupancies at ‘SAGA’ (PM…
(A) TFIIB:Taf1, TFIIA:Taf1, TFIIB:TBP, and TFIIA:TBP occupancy ratios in the Taf1-depletion strain treated or untreated with rapamycin. (B) Log2 change in Pol II occupancy (measured at 0 to +100 bp …
(A) Mean Pol II occupancy averaged over 453 well-transcribed ‘SAGA’ or ‘TFIID’ genes in parental or Taf1-depleted anchor-away strains (black; present study) as well as Taf11- and Taf13-depleted …
List of strains.