Distinct progenitor populations mediate regeneration in the zebrafish lateral line
Abstract
Mechanosensory hair cells of the zebrafish lateral line regenerate rapidly following damage. These renewed hair cells arise from the proliferation of surrounding support cells, which undergo symmetric division to produce two hair cell daughters. Given the continued regenerative capacity of the lateral line, support cells presumably have the ability to replenish themselves. Utilizing novel transgenic lines, we identified support cell populations with distinct progenitor identities. These populations show differences in their ability to generate new hair cells during homeostasis and regeneration. Targeted ablation of support cells reduced the number of regenerated hair cells. Furthermore, progenitors regenerated after targeted support cell ablation in the absence of hair cell damage. We also determined that distinct support cell populations are independently regulated by Notch signaling. The existence of independent progenitor populations could provide flexibility for the continued generation of new hair cells under a variety of conditions throughout the life of the animal.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting files.
Article and author information
Author details
Funding
National Institutes of Health (R21DC015110)
- David W Raible
Hearing Health Foundation (HRP)
- David W Raible
National Institutes of Health (T32GM007270)
- Eric D Thomas
National Institutes of Health (T32HD007183)
- Eric D Thomas
National Institutes of Health (T32DC536115)
- Eric D Thomas
Kellogg Trust
- David W Raible
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Doris K Wu, National Institutes of Health, United States
Ethics
Animal experimentation: This study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All of the animals were handled according to approved institutional animal care and use committee (IACUC) protocolsof the University of Washington.
Version history
- Received: November 17, 2018
- Accepted: February 28, 2019
- Accepted Manuscript published: March 5, 2019 (version 1)
- Version of Record published: March 25, 2019 (version 2)
Copyright
© 2019, Thomas & Raible
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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