Distinct progenitor populations mediate regeneration in the zebrafish lateral line

  1. Eric D Thomas
  2. David W Raible  Is a corresponding author
  1. University of Washington, United States

Abstract

Mechanosensory hair cells of the zebrafish lateral line regenerate rapidly following damage. These renewed hair cells arise from the proliferation of surrounding support cells, which undergo symmetric division to produce two hair cell daughters. Given the continued regenerative capacity of the lateral line, support cells presumably have the ability to replenish themselves. Utilizing novel transgenic lines, we identified support cell populations with distinct progenitor identities. These populations show differences in their ability to generate new hair cells during homeostasis and regeneration. Targeted ablation of support cells reduced the number of regenerated hair cells. Furthermore, progenitors regenerated after targeted support cell ablation in the absence of hair cell damage. We also determined that distinct support cell populations are independently regulated by Notch signaling. The existence of independent progenitor populations could provide flexibility for the continued generation of new hair cells under a variety of conditions throughout the life of the animal.

Data availability

All data generated or analysed during this study are included in the manuscript and supporting files.

Article and author information

Author details

  1. Eric D Thomas

    Department of Biological Structure, University of Washington, Seattle, United States
    Competing interests
    The authors declare that no competing interests exist.
  2. David W Raible

    Department of Biological Structure, University of Washington, Seattle, United States
    For correspondence
    draible@uw.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-5342-5841

Funding

National Institutes of Health (R21DC015110)

  • David W Raible

Hearing Health Foundation (HRP)

  • David W Raible

National Institutes of Health (T32GM007270)

  • Eric D Thomas

National Institutes of Health (T32HD007183)

  • Eric D Thomas

National Institutes of Health (T32DC536115)

  • Eric D Thomas

Kellogg Trust

  • David W Raible

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Animal experimentation: This study was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All of the animals were handled according to approved institutional animal care and use committee (IACUC) protocolsof the University of Washington.

Reviewing Editor

  1. Doris K Wu, National Institutes of Health, United States

Publication history

  1. Received: November 17, 2018
  2. Accepted: February 28, 2019
  3. Accepted Manuscript published: March 5, 2019 (version 1)
  4. Version of Record published: March 25, 2019 (version 2)

Copyright

© 2019, Thomas & Raible

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Eric D Thomas
  2. David W Raible
(2019)
Distinct progenitor populations mediate regeneration in the zebrafish lateral line
eLife 8:e43736.
https://doi.org/10.7554/eLife.43736

Further reading

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    The tooth shape of sharks and mice are regulated by a similar signaling center despite their teeth having very different geometries.