(A, B) Females of indicated genotype who blood-fed (A) or laid >10 eggs given 18 hr to lay (B). n = 6–12 groups/genotype. (C) Embryos/ovary 72 hr post-blood-meal. n = 8–16/genotype. (D–F) Eggs laid by single females of the indicated genotype in the indicated NaCl concentration. Only females laying >10 eggs are presented. In D, lines connect data from individual animals (dots). Median (points) ± 95% confidence interval (bars). n = 65–314 animals/genotype (D) or n = 3–314 animals/genotype (E) per concentration. In F, proportion of animals are binned into three behavioral groups by eggs laid on each substrate. (G–I) Eggs laid in 0 mOsm/kg NaCl by single females of the indicated genotype. n = 30 females/genotype. Blue outline indicates days on which new eggs were laid and histograms indicate mean new eggs (per female). (J) Summary of data in G-I. Mean (line) ±95% confidence interval (shaded bars). In B, data labeled with different letters are significantly different from each other p<0.05, in D,F * indicate difference from wild-type p<0.05 (D) p<0.01 (F); ns = not significant, ANOVA followed by Tukey’s HSD (A, B, E, H), unpaired t-test (C), and chi-squared test corrected for FDR (F). Boxplots in A-C indicate median and 1st and 3rd quartiles, whiskers extend to 1.5x interquartile range.