(a) (left) Susceptibility (TCID50/ml) of miRNA deficient cells (Dgcr8-/-, Dicer-/-) and wild-type parental cells (Dgcr8+/+(ESC1), Dicer+/+(ESC2)) to TMEV infection, higher values represent higher susceptibility (n = 4, p-value<0.05, t-test). (right) Quantification of Influenza A replication after infection of the same cell lines, data show the average (n = 3)±s.d. (*) p-value<0.05 by t-test. (b) Quantification of Ifnb1 expression of ESCs lacking Dgcr8 or Dicer to stimulation with poly(I:C) and G3-YSD. Data show average (n = 3)±s.d., normalized to mock, (*) p-value<0.05 by t-test. (c) Quantification of TMEV vRNA upon JAK1/2 inhibition by Ruxolitinib treatment. Data show the average (n = 3)±s.d. (d) qRT-PCR analyses of ISGs expression after stimulation of wild-type and miRNA-deficient ESCs with IFN-β. Data show average (n = 3)±s.d., normalized to mock treated cells (e, f) Quantification of TMEV replication after infection in Dgcr8 (e) and Dicer (f) parental (+/+), deficient (-/-) and rescued (resc) cell lines. Data are normalized to miRNA-deficient cell lines susceptibility. Data show the average (n = 3)±s.d (*) p-value<0.05 by t-test. Northern blots for three stem-cell specific miRNAs, as control for knock-out and rescue of Dgcr8 and Dicer, are shown at the right of each panel.