Reciprocal action of Casein Kinase Iε on core planar polarity proteins regulates clustering and asymmetric localisation

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Abstract

The conserved core planar polarity pathway is essential for coordinating polarised cell behaviours and the formation of polarised structures such as cilia and hairs. Core planar polarity proteins localise asymmetrically to opposite cell ends and form intercellular complexes that link the polarity of neighbouring cells. This asymmetric segregation is regulated by phosphorylation through poorly understood mechanisms. We show that loss of phosphorylation of the core protein Strabismus in the Drosophila pupal wing increases its stability and promotes its clustering at intercellular junctions, and that Prickle negatively regulates Strabismus phosphorylation. Additionally, loss of phosphorylation of Dishevelled - which normally localises to opposite cell edges to Strabismus - reduces its stability at junctions. Moreover, both phosphorylation events are independently mediated by Casein Kinase Iε. We conclude that Casein Kinase Iε phosphorylation acts as a switch, promoting Strabismus mobility and Dishevelled immobility, thus enhancing sorting of these proteins to opposite cell edges.

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All data generated or analysed during this study are included in the manuscript and supporting files.

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Helen Strutt

Department of Biomedical Science, University of Sheffield, Sheffield, United Kingdom

Competing interests
The authors declare that no competing interests exist.
Jessica Gamage

Department of Biomedical Science, University of Sheffield, Sheffield, United Kingdom

Competing interests
The authors declare that no competing interests exist.
David Strutt

Department of Biomedical Science, University of Sheffield, Sheffield, United Kingdom

For correspondence
d.strutt@sheffield.ac.uk

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0001-8185-4515

Funding

The Wellcome Trust (100986/Z/13/Z)

Helen Strutt
David Strutt

Medical Research Council (G0900203-1/1)

Jessica Gamage

The Wellcome Trust (210630/Z/18/Z)

Helen Strutt
David Strutt

Medical Research Council (G1000405-1/1)

Jessica Gamage

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

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This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.