1. Neuroscience
Download icon

MagC, magnetic collection of ultrathin sections for volumetric correlative light and electron microscopy

  1. Thomas Templier  Is a corresponding author
  1. University of Zurich and ETH Zurich, Switzerland
Tools and Resources
  • Cited 1
  • Views 2,007
  • Annotations
Cite this article as: eLife 2019;8:e45696 doi: 10.7554/eLife.45696

Abstract

The non-destructive collection of ultrathin sections onto silicon wafers for post-embedding staining and volumetric correlative light and electron microscopy traditionally requires exquisite manual skills and is tedious and unreliable. In MagC introduced here, sample blocks are augmented with a magnetic resin enabling remote actuation and collection of hundreds of sections on wafer. MagC allowed the correlative visualization of neuroanatomical tracers within their ultrastructural volumetric electron microscopy context.

Article and author information

Author details

  1. Thomas Templier

    Institute of Neuroinformatics, University of Zurich and ETH Zurich, Zurich, Switzerland
    For correspondence
    thomas.templier2@gmail.com
    Competing interests
    Thomas Templier, A patent application has been filed by ETH Zurich (EP3171150A1)..
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-0523-5947

Funding

ETH Zurich Foundation ETH Grant (42 15-1)

  • Thomas Templier

Innosuisse-Swiss National Foundation Bridge Proof of Concept (173825)

  • Thomas Templier

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Animal experimentation: Animal experiments were approved by the Veterinary office of Canton Zurich (207/2013).

Reviewing Editor

  1. Moritz Helmstaedter, Max Planck Institute for Brain Research, Germany

Publication history

  1. Received: January 31, 2019
  2. Accepted: July 2, 2019
  3. Accepted Manuscript published: July 11, 2019 (version 1)
  4. Accepted Manuscript updated: July 12, 2019 (version 2)
  5. Version of Record published: August 16, 2019 (version 3)

Copyright

© 2019, Templier

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 2,007
    Page views
  • 201
    Downloads
  • 1
    Citations

Article citation count generated by polling the highest count across the following sources: Crossref, PubMed Central, Scopus.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Download citations (links to download the citations from this article in formats compatible with various reference manager tools)

Open citations (links to open the citations from this article in various online reference manager services)

Further reading

    1. Neuroscience
    Víctor J López-Madrona et al.
    Research Article Updated

    Hippocampal firing is organized in theta sequences controlled by internal memory processes and by external sensory cues, but how these computations are coordinated is not fully understood. Although theta activity is commonly studied as a unique coherent oscillation, it is the result of complex interactions between different rhythm generators. Here, by separating hippocampal theta activity in three different current generators, we found epochs with variable theta frequency and phase coupling, suggesting flexible interactions between theta generators. We found that epochs of highly synchronized theta rhythmicity preferentially occurred during behavioral tasks requiring coordination between internal memory representations and incoming sensory information. In addition, we found that gamma oscillations were associated with specific theta generators and the strength of theta-gamma coupling predicted the synchronization between theta generators. We propose a mechanism for segregating or integrating hippocampal computations based on the flexible coordination of different theta frameworks to accommodate the cognitive needs.

    1. Neuroscience
    Kyle Jasmin et al.
    Research Article

    Individuals with congenital amusia have a lifelong history of unreliable pitch processing. Accordingly, they downweight pitch cues during speech perception and instead rely on other dimensions such as duration. We investigated the neural basis for this strategy. During fMRI, individuals with amusia (N=15) and controls (N=15) read sentences where a comma indicated a grammatical phrase boundary. They then heard two sentences spoken that differed only in pitch and/or duration cues, and selected the best match for the written sentence. Prominent reductions in functional connectivity were detected in the amusia group, between left prefrontal language-related regions and right hemisphere pitch-related regions, which reflected the between-group differences in cue weights in the same groups of listeners. Connectivity differences between these regions were not present during a control task. Our results indicate that the reliability of perceptual dimensions is linked with functional connectivity between frontal and perceptual regions, and suggest a compensatory mechanism.