The non-destructive collection of ultrathin sections onto silicon wafers for post-embedding staining and volumetric correlative light and electron microscopy traditionally requires exquisite manual skills and is tedious and unreliable. In MagC introduced here, sample blocks are augmented with a magnetic resin enabling remote actuation and collection of hundreds of sections on wafer. MagC allowed the correlative visualization of neuroanatomical tracers within their ultrastructural volumetric electron microscopy context.
Datasets 1 and 2 are publicly available for online visualization and download at https://neurodata.io/data/templier2019. Code is at https://github.com/templiert/MagC.
- Thomas Templier
- Thomas Templier
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Animal experimentation: Animal experiments were approved by the Veterinary office of Canton Zurich (207/2013).
- Moritz Helmstaedter, Max Planck Institute for Brain Research, Germany
© 2019, Templier
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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