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MagC, magnetic collection of ultrathin sections for volumetric correlative light and electron microscopy

  1. Thomas Templier  Is a corresponding author
  1. University of Zurich and ETH Zurich, Switzerland
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Cite this article as: eLife 2019;8:e45696 doi: 10.7554/eLife.45696

Abstract

The non-destructive collection of ultrathin sections onto silicon wafers for post-embedding staining and volumetric correlative light and electron microscopy traditionally requires exquisite manual skills and is tedious and unreliable. In MagC introduced here, sample blocks are augmented with a magnetic resin enabling remote actuation and collection of hundreds of sections on wafer. MagC allowed the correlative visualization of neuroanatomical tracers within their ultrastructural volumetric electron microscopy context.

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Author details

  1. Thomas Templier

    Institute of Neuroinformatics, University of Zurich and ETH Zurich, Zurich, Switzerland
    For correspondence
    thomas.templier2@gmail.com
    Competing interests
    Thomas Templier, A patent application has been filed by ETH Zurich (EP3171150A1)..
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-0523-5947

Funding

ETH Zurich Foundation ETH Grant (42 15-1)

  • Thomas Templier

Innosuisse-Swiss National Foundation Bridge Proof of Concept (173825)

  • Thomas Templier

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Animal experimentation: Animal experiments were approved by the Veterinary office of Canton Zurich (207/2013).

Reviewing Editor

  1. Moritz Helmstaedter, Max Planck Institute for Brain Research, Germany

Publication history

  1. Received: January 31, 2019
  2. Accepted: July 2, 2019
  3. Accepted Manuscript published: July 11, 2019 (version 1)
  4. Accepted Manuscript updated: July 12, 2019 (version 2)

Copyright

© 2019, Templier

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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