Gene activation by a CRISPR-assisted trans enhancer
Abstract
The deactivated CRISPR/Cas9 (dCas9) is now the most widely-used gene activators. However, the current dCas9-based gene activators are still limited by their unsatisfactory activity. In this study, we developed a new strategy, CRISPR-assisted trans enhancer, for activating gene expression in high efficiency by combining dCas9-VP64/sgRNA with the widely used strong CMV enhancer. In this strategy, a CMV enhancer DNA was recruited to target gene in trans by two systems, dCas9-VP64/csgRNA-sCMV and dCas9-VP64-GLA4/sgRNA-UAS-CMV. The former recruited trans enhancer by the annealing between two short complementary oligonucleotides at the ends of sgRNA and trans enhancer. The latter recruited trans enhancer by the binding between GLA4 fused to dCas9 and UAS sequence of trans enhancer. The trans enhancer activated gene transcription as the natural looped cis enhancer. The trans enhancer could activate both exogenous reporter gene and variant endogenous genes in various cells, with much higher activation efficiency than the current dCas9 activators.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting files.
Article and author information
Author details
Funding
National Natural Science Foundation of China (61571119)
- Jinke Wang
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Irwin Davidson, Institut de Génétique et de Biologie Moléculaire et Cellulaire, France
Version history
- Received: February 10, 2019
- Accepted: April 10, 2019
- Accepted Manuscript published: April 11, 2019 (version 1)
- Version of Record published: April 23, 2019 (version 2)
Copyright
© 2019, Xu et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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