Auto-regulation of Rab5 GEF activity in Rabex5 by allosteric structural changes, catalytic core dynamics and ubiquitin binding

We would like to publish this story in eLife, but feel that its impact would be significantly enhanced by the inclusion of any experiment in cells that would bolster the idea that ubiquitin recognition alone enhances GEF activity. Can you please include any experimental data that would support this conclusion enough to change the title of the story for the broadest readership? Testing these ideas in cells, using mutants that uncouple ubiquitin-dependent membrane recruitment from allosteric activation of the GEF, would add a great deal in terms of the overall significance of the work. We are excited about the story and are confident you can add this without too much difficulty.

We have addressed this request by generating a Rabex5 KO HeLa cell line and expressing full length Rabex5, the RabexΔUBD mutant and the RabexΔLinker mutant as control. The results shown in a new Figure 8 nicely show that loss of Rabex5 causes an alteration of the early endosomal network that can be rescued by low level re-expression of Rabex5. At high levels of expression, Rabex5 causes a gain of function (GOF) phenotype as appearance of enlarged early endosomes. The RabexΔUBD mutant also rescued the Rabex5 KO phenotype at low expression levels and yielded a GOF phenotype at high levels, despite the fact that it was less efficiently recruited to early endosomes than Rabex5 (Figure 8C). This is consistent with the high constitutive GEF activity and lack of UBD contributing to the endosomal localization of this mutant.

In contrast, the RabexΔLinker rescued the Rabex5 KO phenotype, but was much less potent on the stimulation of early endosome size, despite its localization to early endosomes (Figure 8C), consistent with its lower catalytic activity. Altogether, these results indicate that the Rabex5 UBD regulates the association of Rabex5 to early endosomes and its GEF catalytic activity, as its removal leads to a high-level activity despite a low association with early endosomes.

Other comments are for the most part textual and follow here.

1) The Introduction discusses UBD and MIU but the figure shows one bar. Please include both domain names in the text above the red bar so reader can follow more easily. Please name domains on Figure 1, above colored bars. Please make clear that both MIU and UBD are in the same red bar.

Figure 1 has been updated to include both UBD and MIU names. Subsequent figures do not include the distinction between UBD and MIU because throughout the manuscript they are treated as one unit.

2) Please show Figure 1A model in relation to current domain PDB structures used to derive structure model so reader can discern differences. Figure 1B please indicate N- and C-termini.

N- and C-terminal labels were added to Figure 1 and Figure 1—figure supplement 1 was created to illustrate the superimposition of our model on 1TXU.

3) Figure 4 is hard for a non-expert to follow. What differences are being highlighted? What quantity is represented by the scale bar in Figure 4 and Figure 6? Where should the reader look? Figure 5C. Fraction of control rate? Please use a clearer Y axis label. Figure 5A, B; please include the name of the construct on the figure with tetraUb concentration indicated for easy comparison with C.

Multiple changes were made to Figure 4, Figure 5, and Figure 6 to address the points made here. Another panel was added to Figure 4 and to Figure 6 to line up regions within Rabex5 and how they are altered in the different mutants. This makes it clearer for non-experts of Rabex5 structure. Also, information was added in the text to help illustrate which panels in Figure 4 are relevant for each sentence.

4) Parts of the text are repetitive and need to be rewritten for clarity and to eliminate jargon. Some referencing is incomplete or not inserted. We listed some issues but there are more.

[…]

In short, numerous sections of text were removed to address repetition. The references were updated with the Goody review and the Horiuchi error was corrected.

5) Figure 3B and Materials and methods section. It appears that k_cat/K_m is calculated from kinetic data obtained for a single enzyme concentration, so in Figure 3B what is plotted is in fact k_obs/[enzyme concentration]. This is more normally done using enzyme titration. Observed rate constants are plotted as a function of enzyme concentration, the slope is k_cat/K_m and the basal rate is the intersect on the Y-axis.

There is some confusion regarding the accuracy of the axis label in Figure 3. Kinetic data were obtained from a single enzyme concentration. In accordance with previous work in the literature, we labeled these results as k_cat/K_m. However, a reviewer has suggested that this is more accurately referred to as k_obs/[enzyme]. The labels have been updated accordingly.

6) One technical complication is that the reaction starts when Rabex5 is added, and if different proteins have higher or lower GEF activity then by the time of the first measurement the values are already different. This complicates fitting an initial rate to the data, since the top part of the curve is lost. In Figure 3A, Rab has different amounts of bound nucleotide for the different conditions at t0 suggesting this issue is present in the data.

We are aware of this limitation and our analyses include a non-linear curve fit of the data. Thus, the k_obs values do not rely solely on the linear portion of the curve. As such, our k_obs values for the most active enzymes may be slightly underestimated and we state that their activities are “at least x-fold higher” to account for these minor discrepancies. In no way does this alter any of our conclusions.

7) The authors write that they model full-length Rabex5. However, the model in Figure 1B appears to lack the first 20aa and last 42aa if the colour-coded schematic in Figure 1A is correct. Are the white segments in the model in Figure 1B? If the model isn't full-length, then remove the claim from the text.

The coloring schemes used in Figure 1A and 1B were confusing and has been altered to be clearer. The Rabex5 in Figure 1B is indeed a full-length model and we thank you for pointing out this problem.

8) Figure 5, plot rate as a function of Ub-concentration. It would also be helpful to show if this effect is unique to K63 chains and what effect mono-ubiquitin has.

Another panel was added to Figure 5 to show the non-corrected data (Figure 5D). Due to the activity differences between the WT Complex and RabexΔUBD Complex, we chose to normalize the activities. The normalized data is still included, but may be redundant. We also tested Lys64 linked tetra-Ubiquitin as well as a linear construct of di-Ubiquitin. A description of those results has been added to the text. The linear construct did not alter Rabex5 activity. Thus, mono-Ubiquitin would likely have no activity.

9) Exploratory HDX-MS results to select RabexΔ82-117 for use is a bit vague. Can the authors be more specific about why this region was selected? Apparently, a larger region was slightly destabilizing with respect to deuterium uptake, but it isn't clear if shorter deletions were tested and what the results were.

We added text to help describe our thought processes behind making the Linker deletion mutants. Only two deletion constructs were made because the exploratory HDX-MS results which guided the design were unable to isolate the activity to a smaller region within the linker. We didn’t want to embark on constructing mutants without a rational reason.

10) Would the differences in the orientation of the helical bundle relative to the catalytic core be expected to directly impact the accessibility of the catalytic site to Rab5 or is it more likely that the differences in GEF activity are a consequence of conformational changes in the substrate site of the Vps9 domain?

We have added a sentence stating that we believe this not the case as the 4-HB is likely on the opposite side of the protein. Thus, alterations in activity are likely due to conformational changes in the Vps9 domain rather than some sort of competition.

11) Indicate the type of residue being cross-linked (e.g. cysteine).

The name of our cross-linking reagent is listed in the Materials and methods section. It reacts with Lys.