Imaging of glucose metabolism by 13C-MRI distinguishes pancreatic cancer subtypes in mice
- NCI, NIH, United States
- Urologic Oncology Branch, Center for Cancer Research, NCI, NIH, United States
- Hokkaido University, Japan
- JST, PREST, Japan
- NIH, United States
- University of Kentucky, United States
- GE HealthCare, United States
- Technical University of Denmark, Denmark
Figures
Figure 1 with 3 supplements
Distinct anatomical microstructure differences exist between Hs 766T and MIA Paca-2 xenografts while CE-MS shows only subtle metabolic differences.
(A and C) T2 weighted anatomical RARE images of (A) Hs 766T and (C) MIA Paca-2 PDAC xenografts implanted on the left leg. Focal necrosis is evident in the Hs 766T tumor, but not in the MIA Paca-2 …
Figure 1—figure supplement 1
Protein expression levels from immunoblotting of tumor extracts of key proteins associated with metabolism.
Su86.86 forms a distinct subtype with statistically significant differences of the glucose transporter one and the angiogenic factor CD31. Error bars represent standard deviation (n = 4).
Figure 1—figure supplement 2
Metabolite differences between normal tissue and the MIA Paca-2 PDAC leg xenografts as analyzed by CE/MS.
White boxes indicate metabolites not detected. Gray boxes indicate a statistically insignificant difference between cell lines (two-sided t-test, corrected for multiple comparisons by the two-stage …
Figure 1—figure supplement 3
Metabolic differences within glycolysis between normal tissue and MIA Paca-2 and Hs 766T PDAC leg xenografts in terms of absolute concentrations (nmol/g tumor wet weight).
Differences between normal tissue and cancerous can be seen throughout. Hs 766T and MIA Paca-2 diverge after the pentose phosphate shunt at glyceraldehyde-3-phosphate.
Figure 2
Pyruvate metabolism is similar in Hs 766T and MIA Paca-2 Xenografts.
(A and B) Representative signal after injecting 300 µL of 98 mM hyperpolarized [1-13C] pyruvate into the tail vein of a mouse of a nude mouse with either a (A) Hs 766T or (B) MiaPaCa2 leg xenograft. …
Figure 3 with 1 supplement
Glucose metabolism differentiates Hs 766T and MiaPaca2 xenografts.
(A) Representative signal before and 1 hr after injecting 50 mg of [U-13C] glucose into the tail vein of a mouse of a nude mouse with a MIA Paca-2 leg xenograft. The largest signal at 32 ppm is due …
Figure 3—figure supplement 1
Ex vivo NMR analysis of Hs766T and MIA Paca-2 xenografts.
(A) Representative multiplicity edited HSQC from the polar fraction of a Hs 766T xenograft. The sign of the peak is reflective of the proton connectivity of the carbon: positive peaks (red) arise …
Figure 4
CSI imaging of a Hs 766T mouse leg xenograft after a 50 mg [U-13C] glucose injection in a volume of 300 microliters of PBS.
An 8 × 8 image of the tumor bearing mouse leg was acquired by chemical shift imaging every 48 s for 60 min. The final image was zero-filled to 16 × 16. Each is voxel 0.15 cm x 0.15 cm x 1.6 cm in …
Figure 5
Contour maps created from time averages of the peak maxia of the glucose and lactate signals for three representative MIA Paca-2 (left) and Hs 766T (right) tumors.
https://doi.org/10.7554/eLife.46312.010Additional files
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