(A) Correlation of TP63 and NRG1 in LUSC (n = 223) and Esophageal (n = 263) patient samples in TCGA data set. Statistical significance was determined by two-tailed test and Pearson correlation (r) …
(A) NRG1 and ERBB3 mRNA levels in head and neck squamous carcinoma (HNSC), lung squamous carcinoma (LUSC) and lung adenocarcinoma (LUAD) patient samples from TCGA. (B) In vitro growth of different …
Effect of anti-NRG1 or anti-Ragweed (control) antibodies in (A) FaDu (head and neck), HCC95 (lung) and KYSE-180 (esophagus) SCC cell lines in preclinical mouse xenograft models, N = 8–12 mice/group, …
Effect of anti-NRG1 or anti-Ragweed (control) antibodies in FaDu (head and neck), HCC95 (lung) and KYSE-180 (esophagus) SCC cell lines in preclinical mouse xenograft models, N = 8–12 mice/group. …
(A) In vitro growth of ovarian cell lines in the presence of anti-NRG1 or anti-Ragweed (control) antibody. Expression of NRG1, Erbb3, Erbb4 and TP63 by RNAseq are shown below the respective in vitro …
(A) Representative image of KRT10 (differentiation marker) staining of tumor from FaDu and HCC95 SCC models upon anti-NRG1 treatments compared to anti-Ragweed control. N = 5 mice/group. (B) Protein …
Panel of human airway basal cell gene signature in Figure 4C (as published by Hackett et al., 2011).
Representative image of H and E staining of KYSE-180, HCC95 and FaDu tumors upon anti-NRG1 treatments compared to anti-Ragweed control. N = 5 mice/group.
Expression of proliferation, differentiation and progenitor cell related markers following one dose of anti-NRG1 relative to anti-Ragweed treatment in HCC95 lung SCC xenograft tumors by RNAseq. …
Relative expression of differentiation markers such as KRT1, KRT10, IVL and KRTDAP in FaDu, HCC95 and KYSE-180 cell lines after treatment with antibodies for 3 days in vitro.
Reagent type (species)or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Strain, strainbackground (M. musculus) | C.B-17 SCID beige mice | Charles River Labs | CB17.Cg-PrkdcscidLystbg-J/Crl | |
Strain, strainbackground (M. musculus) | Athymic nude mice | Harlan Sprague Dawley (Livermore facility) | Hsd:Athymic Nude-Foxn1nu | |
Genetic reagent (M. musculus) | Lgr5CreERT2+; tdTomato; KrasG12Dwt/ki; PTENloxP/loxP | Lgr5CreERT2+; pubmed id:21927002 tdTomato; pubmed id: PMC2840225 KrasG12Dwt/ki; pubmed id: 11751630 PTENloxP/loxP; pubmed id: 11691952 | LAR, Genentech | |
Antibody | Anti-Ragweed | Genentech | 9652 | In vivo: 20 mg/kg, i.p.,qwk In vitro: 20 ug/ml |
Antibody | Anti-NRG1 | Genentech | YW538.24.71 | In vivo: 20 mg/kg, i.p.,qwk In vitro: 20 ug/ml |
Antibody | deltaNTP63 (Rabbit Polyclonal IgG) | Biolegend | RRID:AB_2256361 Cat. #: 619001 | WB (1:1000) |
Antibody | Actin (Mouse IgG) | BD Bioscience | RRID:AB_2289199 Cat. #: 612656 | WB (1:5000) |
Antibody | p-ERBB3 (Rabbit mAb) | Cell Signaling Technologies | RRID:AB_2099709 Cat. #: 4791 | WB (1:500) |
Antibody | ERBB3 (Rabbit mAb) | Cell Signaling Technologies | RRID:AB_2721919 Cat. #: 12708 | WB (1:500) |
Antibody | Rabbit PARP (Rabbit polyclonal) | Cell Signaling Technologies | RRID:AB_2160739 Cat. #: 9542 | WB (1:1000) |
Antibody | Cleaved caspase-3 (Rabbit mAb) | Cell Signaling Technologies | Cat. #: 9664 | WB (1:1000) |
Antibody | TP63 alpha (Rabbit mAb) | Cell Signaling Technologies | RRID:AB_2637091 Cat. #: 13109 | CHIP (1:100) WB (1:1000) |
Antibody | KRT10 (Rabbit polyclonal) | Covance Biologicals | RRID:AB_291580 Cat. #: PRB-159P | IHC (1:1000) |
Commercial assay or kit | TP63-all | Life Technologies | Hs00978340_m1 | qPCR |
Commercial assay or kit | TA-TP63-TA | Life Technologies | Hs00186613_m1 | qPCR |
Commercial assay or kit | deltaN-TP63 | Life Technologies | Hs00978339_m1 | qPCR |
Commercial assay or kit | NRG1a | Life Technologies | Hs01103794_m1 | qPCR |
Commercial assay or kit | NRG1b | Life Technologies | Hs00247624_m1 | qPCR |
Commercial assay or kit | KRTDAP | Life Technologies | Hs00415563_m1 | qPCR |
Commercial assay or kit | KRT1 | Life Technologies | Hs00196158_m1 | qPCR |
Commercial assay or kit | KRT10 | Life Technologies | Hs00166289_m1 | qPCR |
Commercial assay or kit | IVL | Life Technologies | Hs00846307_s1 | qPCR |
Other | TA-TP63 | Dharmacon | #13 = J-003330–13 | siRNA |
Other | all isoform-TP63 | Dharmacon | #14 = J-003330–14 | siRNA |
Other | NTC#3 | Dharmacon | D-001810–03 | siRNA |
Other | NTC#4 | Dharmacon | D-001810–04 | siRNA |
Software,algorithm | GraphPad Prism | graphpad.com | RRID:SCR_002798 |