(a) MukBEF self-organises via a Turing instability into regions of high local density (foci). The position of these foci is determined by the balancing of fluxes originating from the well-mixed cytosolic state. With spatially homogeneous loading of the well-mixed state onto the nucleoid, the fluxes balance, in the case of a single focus, at the mid-cell position (i). An object (the ori) that is attracted up the resulting gradient will also be positioned at mid-cell. However, if loading is not homogeneous, then the position at which the fluxes balance changes (ii). If higher loading occurs at the ori, then the mutual attraction between the self-organised MukBEF focus and the ori results in the suppression of fluctuations, strong colocalisation and positioning at mid-cell (iii). The system is analogous to a compound spring with the fixed end at mid-cell (but with a non-trivial relationship between the spring constants and preferential loading ratio). At high levels of preferential loading, colocalised MukBEF-ori becomes less sensitive to the (decreased) incoming flux from other regions, and is therefore less attracted towards mid-cell, resulting in more diffusive behaviour and a broader position distribution. (b) Suppose both ori have been pulled towards the same MukBEF focus. Due to the flux imbalance, the MukBEF focus without an ori will be effectively pulled towards the other one (i) until the fluxes balance (ii). The stochastic nature of ori and MukBEF movement means that at some point one ori leaves the influence of its MukBEF focus and becomes associated to the other focus (iii). This is aided by the fact that the two MukBEF foci repulse each other so that the first MukBEF focus is prevented from following the ori. After the ori associates to the other MukBEF focus, the balance of fluxes is again disturbed and MukBEF-ori move as single units towards opposite quarter positions (iv). Due the strong association and reduced spatial fluctuations caused by preferential loading, this is the stable configuration and the system does not revert back to (i). See also Figure 6.