Lineage does not regulate the sensory synaptic input of projection neurons in the mouse olfactory bulb
- California Institute of Technology, United States
Figures
Figure 1 with 3 supplements
Clonal analysis of projection neurons using Nestin-CreERT2::Confetti mice to sparsely label neuronal progenitors.
(A) Schematic representation of the olfactory bulb (OB). Axons from olfactory sensory neurons (OSNs) expressing the same receptor project to a single glomerulus, forming synaptic contacts with the …
Figure 1—figure supplement 1
M/T and pyramidal neurons labeled with different fluorescent proteins.
(A–C) Confocal images of three M/T cells and (D–F) three pyramidal neuron clones labeled with different fluorescent proteins in OB and neocortex in coronal sections of P21 mice treated with TMX at …
Figure 1—figure supplement 2
Labeling of progenitor cells at E10.5 in a Nestin-Cre::Ai9 mouse.
(A, B) Confocal images of two serial sagittal sections through the brain of an E10.5 Nestin-Cre:Ai9 mouse labeled with Tbr2 antibody (green in A) which is restricted to the pallium, and with the …
Figure 1—figure supplement 3
M/T cells labeled at P7 in a Nestin-Cre::Ai9 mouse.
(A) Sagittal sections through the OB of a P7 Nestin-Cre::Ai9 mouse labeled with Tbx21 antibody (green) to identify M/T cells. (A1–A3) High-magnification images of different positions of the main …
Figure 2 with 1 supplement
Sparse labeling of progenitor cells in the embryonic mouse brain.
(A–D) Sagittal sections through the brain of an E12.5 mouse treated with TMX at E10.5. (A–B) Confocal images of individual clones labeled in the OB expressing cRFP (A–A’) and cYFP (B–B’). (A’–B’) …
Figure 2—figure supplement 1
Progenitor cells labeled in neocortex with three different fluorescent proteins.
(A–C) Confocal images of single clones labeled in the neocortex with cYFP (A), cRFP (B) and mCFP (C) in brain sagittal sections of E12.5 mice treated with TMX at E10.5. (A’–C’) High-magnification …
Figure 3 with 4 supplements
Clone size and distribution of cells labeled in the olfactory bulb and neocortex.
(A) Clone size quantification in the OB and neocortex. Data are shown as average showing all data points. (B–D) 3D reconstruction of a NestinCreERT2::Confetti P21 mice OB (B–C) and neocortex (D) …
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Figure 3—source data 1
Quantification of the cell NNDs in real and randomized OB and neocortex clones at E10.5.
- https://doi.org/10.7554/eLife.46675.013
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Figure 3—source data 2
Quantification of the cell NNDs in real OB and neocortex clones at E12.5.
- https://doi.org/10.7554/eLife.46675.014
Figure 3—figure supplement 1
3D reconstruction of clones labeled in the olfactory bulb.
(A–J) 3D reconstructions from 11 OBs showing the 14 OB clones analyzed in Figure 3. (A–J) Frontal and (A’–J’) lateral views of the OBs. (K) Division of the OB in the approximate NQO1 (purple) and …
Figure 3—figure supplement 2
3D reconstruction of clones labeled in the neocortex.
(A–F) 3D reconstructions from the six clones analyzed in the neocortex. Clone size is indicated below each 3D reconstruction. Scale bar in F is 1 mm and applies to A-F. Orientation of diagrams: D, …
Figure 3—figure supplement 3
NND distribution of single clones based on their cell number.
(A) NND cumulative percentage of individual clones analyzed in the OBs (n = 14). Red line represents the NND average of all clones analyzed, while dark and light blue represents the NND of single …
Figure 3—figure supplement 4
Clone size and distribution of cells labeled in the olfactory bulb and neocortex when TMX was administered at E12.5.
(A) Experimental design to label neuronal progenitors with tamoxifen (TMX) at embryonic day 12 (E12.5), and analyzed at postnatal day 21 (P21). (B) Clone size quantification in the OB and neocortex. …
Figure 4 with 2 supplements
Connectivity of clonally related M/T cells when TMX was administered at E10.5.
(A) Confocal images of four sister M/T cells belonging to a putative individual clone in the OB. (B–E) Confocal images of sister M/T cells from four clones, in four different OBs, with their somata …
Figure 4—figure supplement 1
Connectivity of clonally related M/T cells when TMX was administered at E12.5.
(A) Confocal images of clonally related M/T cells belonging to a putative individual clone in the OB after TMX administration at E12.5. (B) Confocal image of two sister cells with their somata close …
Figure 4—figure supplement 2
Connectivity of clonally related M/T cells in the AOB.
(A–C) Confocal images of M/T cells from three different clones (A, B, and C) in the accessory olfactory bulb (AOB) after TMX administration at E10.5. (A’–C’) Schematic representation of the confocal …
Author response image 1
Axons in olfactory cortical regions.
(A-F) Confocal images of the axons in the lateral olfactory tract (LOT), in two different brains, at different anterior-posterior levels of the brain in coronal sections. (A-C) Axons in the LOT at …
Author response image 2
Volume for random dataset in the olfactory bulb and neocortex.
(A-C) 3D reconstruction from clones observed in OB (A-B) and neocortex (D) on our E10.5 experiment. (D-F) 3D reconstruction used to generate the random data set in the OB (D-E) and the neocortex (F).
Additional files
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Supplementary file 1
M/T cells clones in the accessory olfactory bulb (A) Summary of all M/T cells clones observed in the accessory olfactory bulb (AOB), indicating the number of cells found in the AOB and the fluorescent protein expressed by M/T cells in the AOB and main OB.
- https://doi.org/10.7554/eLife.46675.018
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Transparent reporting form
- https://doi.org/10.7554/eLife.46675.019
