Classical conditioning drives learned reward prediction signals in climbing fibers across the lateral cerebellum
- Duke University School of Medicine, United States
Figures
Figure 1
Appetitive classical conditioning regime for head-fixed mice.
(A) Diagram of headplate and 3 mm cranial window. (B) Scale representation of the 3 mm cranial windows over either Lobule Simplex (LS) or Crus I and II. (C) Mice viewed a static full screen vertical …
Figure 2
Single photon, mesoscale imaging of Cspk activity during behavior.
(A) Schematic of the cerebellar cortex with GCaMP6f expressed in PCs for single or two photon dendrite imaging. (B) Average ΔF/F timecourse (black, collected at 10 Hz) and lick rate (blue bars) for …
Figure 3 with 3 supplements
Cspk modulation in LS across learning.
(A) ΔF/F timecourses from an example neuron measured via two-photon calcium imaging for the first day of training in naïve mice. Gray traces are the first 50 trials and the black trace is the …
Figure 3—figure supplement 1
Comparison of the mean ΔF/F response for all neurons segregated according to lobule, pre vs post learning, and pre vs post reward delivery. (A-C) (Schematic indicating imaging location (left) and summary of ΔF/F responses before and after learning for each area (right).
https://doi.org/10.7554/eLife.46764.005
Figure 3—figure supplement 2
A minority of PC dendrites can respond to both reward and the reward-predictive cue after learning.
(A) Mean cue-aligned PSTH for PC dendrites that exhibited Cspk responses to reward. (B) Mean cue-aligned PSTH for PC dendrites that did not exhibit Cspk responses to reward.
Figure 3—figure supplement 3
No evidence of Cspk suppression following reward omission.
(A–C) Cspk responses identified as suppressed below baseline according to statistical criteria (Materials and methods) in LS, Crus I and Crus II show no suppression upon visual inspection. These …
Figure 4
Cspk responses measured in the same fields of view across learning.
(A) Example of manual identification from a field of view containing several of the same PC dendrites on day one and day N+1. Left, fields of view for day 1 (top) and day N+1 (bottom). Right, …
Figure 5
Cspk modulation in Crus I across learning.
(A) ΔF/F timecourses from an example neuron measured via two-photon calcium imaging for the first day of training in naïve mice. Gray traces are the first 50 trials and the black trace is the …
Figure 6
Cspk modulation in Crus II across learning.
(A) ΔF/F timecourses from an example neuron measured via two-photon calcium imaging for the first day of training in naïve mice. Gray traces are the first 50 trials and the black trace is the …
Figure 7
Body movements do not explain the learned reward predictive Cspks.
(A) Piezoelectric movement sensor was used to measure movements during the behavior for a subset of animals. (B) movement traces corresponding to mouse breathing during awake (top) and anesthetized …
Additional files
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Source data 1
Data organization files.
Contains three files: A readme file that describes how the data are organized. "expNums_2P" and "expNums_LS_piezo" contain descriptive information about the two-photon experiments. expNums_2P is a 1x3 structure where each dimension is a different behavior condition (1=day1, 2=dayN+1, 3=dayN+2). The subfields contain information about which mice were imaged on each day, which lobules were imaged, and how many neurons were in the field of view. The file expNums_LS_piezo contains the same information but only for the lobule simplex datasets collected with the piezo movement data.
- https://doi.org/10.7554/eLife.46764.012
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Source data 2
Widefield data.
- https://doi.org/10.7554/eLife.46764.013
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Source data 3
Two photon LS piezo data.
- https://doi.org/10.7554/eLife.46764.014
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Source data 4
Two photon C1.
- https://doi.org/10.7554/eLife.46764.015
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Source data 5
Two photon C2.
- https://doi.org/10.7554/eLife.46764.016
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Source data 6
Two photon LS data.
Day_N1 (1).
- https://doi.org/10.7554/eLife.46764.017
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Source data 7
Two photon LS data.
Day_N1 (2).
- https://doi.org/10.7554/eLife.46764.018
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Source data 8
Two photon LS data.
Day_1 (1).
- https://doi.org/10.7554/eLife.46764.019
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Source data 9
Two photon LS data.
Day_1 (2).
- https://doi.org/10.7554/eLife.46764.020
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Source data 10
Two photon LS data.
Day_N2.
- https://doi.org/10.7554/eLife.46764.021
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Transparent reporting form
- https://doi.org/10.7554/eLife.46764.022
