WT or IFNAR KO BMDM were stimulated with heat-killed bacteria, with the addition of IFN-β after one hour of stimulation, where indicated. (A) Induced gene expression was measured by qPCR and fold change was calculated compared to unstimulated BMDM. Data are shown for the timepoint of maximum WT expression over a 6 hr time course: 2 hr Nfil3, 4 hr Batf2, 4 hr Themis2, 2 hr Cish, 4 hr Chd1, and 2 hr Rcan1. Data shown are from two independent experiments. (B) After 6 hr of stimulation, supernatants were removed and replaced with fresh medium for a subsequent 6 hr of stimulation. The 6–12 hr supernatants were analyzed by cytometric bead array. Cytokine data are pooled from four independent experiments (n = 4, n = 3, and n = 3 for WT, WT + IFN-β, and IFNAR KO, respectively). Stars represent statistical significance based on ordinary two-way ANOVA, comparing WT + IFN-β or IFNAR KO treatment groups to WT for each respective bacterial stimulation (*p≤0.05).