1. Cell Biology
  2. Structural Biology and Molecular Biophysics

Structural state recognition facilitates tip tracking of EB1 at growing microtubule ends

  1. Taylor A Reid
  2. Courtney Coombes
  3. Soumya Mukherjee
  4. Rebecca R Goldblum
  5. Kyle White
  6. Sneha Parmar
  7. Mark McClellan
  8. Marija Zanic
  9. Naomi Courtemanche
  10. Melissa K Gardner  Is a corresponding author
  1. University of Minnesota, United States
  2. Vanderbilt University, United States
https://doi.org/10.7554/eLife.48117
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Cite this article
  1. Taylor A Reid
  2. Courtney Coombes
  3. Soumya Mukherjee
  4. Rebecca R Goldblum
  5. Kyle White
  6. Sneha Parmar
  7. Mark McClellan
  8. Marija Zanic
  9. Naomi Courtemanche
  10. Melissa K Gardner
(2019)
Structural state recognition facilitates tip tracking of EB1 at growing microtubule ends
eLife 8:e48117.
https://doi.org/10.7554/eLife.48117
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Abstract

The microtubule binding protein EB1 specifically targets the growing ends of microtubules in cells, where EB1 facilitates the interactions of cellular proteins with microtubule plus-ends. Microtubule end targeting of EB1 has been attributed to high affinity binding of EB1 to GTP-tubulin that is present at growing microtubule ends. However, our 3D single-molecule diffusion simulations predicted a ~6000% increase in EB1 arrivals to open, tapered microtubule tip structures relative to closed lattice conformations. Using quantitative fluorescence, single-molecule, and electron microscopy experiments, we found that the binding of EB1 onto opened, structurally disrupted microtubules was dramatically increased relative to closed, intact microtubules, regardless of hydrolysis state. Correspondingly, in cells, the blunting of growing microtubule plus-ends by Vinblastine was correlated with reduced EB1 targeting. Together, our results suggest that microtubule structural recognition, based on a fundamental diffusion-limited binding model, facilitates the tip tracking of EB1 at growing microtubule ends.

Data availability

All data generated or analysed during this study are included in the manuscript and supporting files.

Article and author information

Author details

  1. Taylor A Reid

    Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, United States
    Competing interests
    The authors declare that no competing interests exist.

  2. Courtney Coombes

    Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, United States
    Competing interests
    The authors declare that no competing interests exist.

  3. Soumya Mukherjee

    Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, United States
    Competing interests
    The authors declare that no competing interests exist.

  4. Rebecca R Goldblum

    Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, United States
    Competing interests
    The authors declare that no competing interests exist.

  5. Kyle White

    Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, United States
    Competing interests
    The authors declare that no competing interests exist.

  6. Sneha Parmar

    Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, United States
    Competing interests
    The authors declare that no competing interests exist.

  7. Mark McClellan

    Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, United States
    Competing interests
    The authors declare that no competing interests exist.

  8. Marija Zanic

    Department of Cell and Developmental Biology, Vanderbilt University, Nashville, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-5127-5819

  9. Naomi Courtemanche

    Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, United States
    Competing interests
    The authors declare that no competing interests exist.

  10. Melissa K Gardner

    Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, United States
    For correspondence
    klei0091@umn.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-5906-7363

Funding

National Institutes of Health (R01-GM103833)

  • Melissa K Gardner

National Institutes of Health (R35-GM126974)

  • Melissa K Gardner

National Science Foundation (1350741)

  • Melissa K Gardner

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Andrew P Carter, MRC Laboratory of Molecular Biology, United Kingdom

Version history

  1. Received: May 1, 2019
  2. Accepted: August 23, 2019
  3. Accepted Manuscript published: September 3, 2019 (version 1)
  4. Version of Record published: September 12, 2019 (version 2)

Copyright

© 2019, Reid et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Taylor A Reid
  2. Courtney Coombes
  3. Soumya Mukherjee
  4. Rebecca R Goldblum
  5. Kyle White
  6. Sneha Parmar
  7. Mark McClellan
  8. Marija Zanic
  9. Naomi Courtemanche
  10. Melissa K Gardner
(2019)
Structural state recognition facilitates tip tracking of EB1 at growing microtubule ends
eLife 8:e48117.
https://doi.org/10.7554/eLife.48117

Share this article

https://doi.org/10.7554/eLife.48117

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