A critical role of VMP1 in lipoprotein secretion
Abstract
Lipoproteins are lipid-protein complexes that are primarily generated and secreted from the intestine, liver, and visceral endoderm and delivered to peripheral tissues. Lipoproteins, which are assembled in the endoplasmic reticulum (ER) membrane, are released into the ER lumen for secretion, but its mechanism remains largely unknown. Here, we show that the release of lipoproteins from the ER membrane requires VMP1, an ER transmembrane protein essential for autophagy and certain types of secretion. Loss of vmp1, but not other autophagy-related genes, in zebrafish causes lipoprotein accumulation in the intestine and liver. Vmp1 deficiency in mice also leads to lipid accumulation in the visceral endoderm and intestine. In VMP1-depleted cells, neutral lipids accumulate within lipid bilayers of the ER membrane, thus affecting lipoprotein secretion. These results suggest that VMP1 is important for the release of lipoproteins from the ER membrane to the ER lumen in addition to its previously known functions.
https://doi.org/10.7554/eLife.48834.001Introduction
Lipoproteins are lipid-protein complexes whose main function is to transport hydrophobic lipids derived from dietary and endogenous fat to peripheral tissues by the circulation systems for energy utilization or storage. Lipoproteins are primarily formed in and secreted from the intestine, liver, and visceral endoderm (Farese et al., 1996; Sirwi and Hussain, 2018). Lipoproteins are composed of a neutral lipid core (triglycerides and cholesterol esters) surrounded by a phospholipid monolayer and proteins (called apolipoproteins). At an early stage in lipoprotein assembly, neutral lipids are synthesized and accumulate within the lipid bilayer of the endoplasmic reticulum (ER) membrane (Demignot et al., 2014; Sundaram and Yao, 2010; Tiwari and Siddiqi, 2012; Yen et al., 2015). These lipid structures are associated with apolipoprotein B (APOB), a major protein constituent of lipoproteins, co-and/or post-translationally (Davidson and Shelness, 2000). This step requires microsomal triglyceride-transfer protein (MTTP), an ER luminal chaperone that interacts, stabilizes, and lipidates APOB (Sirwi and Hussain, 2018). Then, lipoproteins are released into the ER lumen (Demignot et al., 2014; Sundaram and Yao, 2010; Tiwari and Siddiqi, 2012; Yen et al., 2015) and transported to the Golgi for secretion. A key long-standing question is how lipoproteins bud off from the ER membrane to the ER lumen, which remains largely unknown.
Vacuole membrane protein 1 (VMP1), which was originally identified as a pancreatitis-associated protein, is a multispanning membrane protein in the ER (Dusetti et al., 2002; Vaccaro et al., 2003). VMP1 (EPG-3 in Caenorhabditis elegans) is essential for autophagosome formation in mammals (Itakura and Mizushima, 2010; Ropolo et al., 2007; Tian et al., 2010), Dictyostelium (Calvo-Garrido et al., 2008), and Caenorhabditis elegans (Tian et al., 2010). Although VMP1 may regulate the PI3K complex I signal (Calvo-Garrido et al., 2014; Kang et al., 2011; Ropolo et al., 2007), which is required for autophagy (Ktistakis and Tooze, 2016; Mizushima et al., 2011; Nakatogawa et al., 2009; Søreng et al., 2018), VMP1 also controls ER contact with other membranes, including autophagic membranes (Tábara and Escalante, 2016; Zhao et al., 2017), by regulating the calcium pump sarcoendoplasmic reticulum calcium transport ATPase (SERCA) (Zhao et al., 2017) and ER contact proteins VAPA and VAPB (Zhao et al., 2018). At the ER-autophagic membrane contact sites, VMP1 forms ER subdomains enriched in phosphatidylinositol synthase (Tábara et al., 2018), which could serve as the initiation site of autophagosome formation (Nishimura et al., 2017).
In addition to the involvement in autophagy, VMP1 is known to be required for the secretion of soluble proteins that are transported via the ER-to-Golgi trafficking pathway. In Drosophila S2 cells, VMP1 (identified as TANGO5) is important for constitutive secretion and Golgi organization (Bard et al., 2006). In Dictyostelium, VMP1 is required for secretion of specific proteins such as α-mannosidase and a cysteine proteinase and maintenance of organelle homeostasis (Calvo-Garrido et al., 2008).
Physiologically, VMP1 is essential for survival under hypoosmotic and starvation conditions in Dictyostelium (Calvo-Garrido et al., 2008) and Caenorhabditis elegans (Tian et al., 2010), respectively. However, its physiological roles in vertebrates remain unknown. Recent studies in human cells (Morita et al., 2018; Tábara and Escalante, 2016; Zhao et al., 2017) and Caenorhabditis elegans (Zhao et al., 2017) revealed that neutral lipid-containing structures accumulate in VMP1-depleted cells, suggesting the function of VMP1 in lipid metabolism. In this study, via deletion of the VMP1 gene, we found that VMP1 is essential for survival during the larval and early embryonic periods in zebrafish and mice, respectively. We further revealed that VMP1 is important for lipoprotein release from the ER membrane into the lumen to be secreted from the intestine, liver, and visceral endoderm. This function is distinct from previously known functions of VMP1 in autophagy and secretion.
Results
Loss of vmp1 in zebrafish causes larval lethality and defects in autophagy
To reveal the physiological functions of VMP1 in vertebrates, we used zebrafish and mice. We generated vmp1-deficient zebrafish using the CRISPR/Cas9 system. A frameshift mutation was introduced into exon 6 of the vmp1 gene (Figure 1A). Gross examination revealed that the abdominal part was less transparent in vmp1-/- zebrafish at 6 days post fertilization (dpf), indicating the presence of abnormal deposits (Figure 1B). We also noticed that the swimbladder was not inflated in vmp1-/- zebrafish, which will be described in more detail elsewhere. All vmp1-/- zebrafish died around at nine dpf (Figure 1C), suggesting that VMP1 is essential for survival during the larval period.
-
Figure 1—source data 1
- https://doi.org/10.7554/eLife.48834.003
Autophagy was defective in vmp1-/- zebrafish; many large LC3 puncta accumulated in several tissues, including the brain, spinal cord, and skeletal muscles, which were abnormal autophagy-related structures typically observed in VMP1-deficient mammalian cells (Itakura and Mizushima, 2010; Kishi-Itakura et al., 2014; Zhao et al., 2017) (Figure 1D). An increase in the levels of the lipidated form of LC3 (LC3-II) was also observed in vmp1-/- zebrafish (Figure 1E), as previously observed in VMP1-deficient mammalian cells (Itakura and Mizushima, 2010; Morita et al., 2018; Shoemaker et al., 2019; Zhao et al., 2017). These results suggest that autophagic flux is blocked in vmp1-/- zebrafish.
Accumulation of neutral lipids in intestinal epithelial cells and hepatocytes in vmp1-deficient zebrafish
The abnormal deposits in the abdomen were observed in all vmp1-/- zebrafish (n = 11), but not in vmp1+/- (n = 30) or vmp1+/+ zebrafish (n = 7). These deposits resemble neutral lipid accumulation in the intestine (Hölttä-Vuori et al., 2010). Indeed, the deposits in vmp1-/- zebrafish were stained with oil red O, a neutral lipid-soluble dye (Figure 2A). Oil red O staining of cross sections and electron microscopy revealed that, in vmp1-/- zebrafish, large neutral lipid-containing structures accumulated in intestinal epithelial cells and hepatocytes (Figure 2B,C) but not in other organs, including the brain (Figure 2—figure supplement 1A,B) and skeletal muscles (Figure 2—figure supplement 1A,C). Accumulation of large lipid-containing structures was not observed in zebrafish lacking the rb1cc1/fip200 (Figure 2—figure supplement 1D) or atg5 (Figure 2—figure supplement 1E) gene, both of which are required for autophagy (Hara et al., 2008; Mizushima et al., 2001). These results suggest that neutral lipids accumulate in vmp1-/- zebrafish, and that this lipid phenotype is not caused by deficient autophagy.
Loss of Vmp1 in mice causes early embryonic lethality and accumulation of lipids in visceral endoderm cells
To elucidate the physiological functions of VMP1 in mammals, Vmp1-deficient mice were generated using an embryonic stem (ES) cell line carrying a gene-trap cassette downstream of exon 3 of the Vmp1 gene (Figure 3—figure supplement 1A). Heterozygous Vmp1gt/+ mice were healthy and phenotypically indistinguishable from wild-type littermates. In contrast, Vmp1gt/gt embryos were embryonic lethal; they were detected at 7.5 days postcoitum (dpc) but not after 9.5 dpc (Figure 3A). Vmp1gt/gt embryos at 7.5 dpc were smaller than wild-type embryos and accumulated the autophagy substrate p62 (Figure 3B), suggesting that VMP1 is important for early embryonic development as well as autophagy in mice.
-
Figure 3—source data 1
- https://doi.org/10.7554/eLife.48834.008
The visceral endoderm is an extraembryonic layer critical for maternal-to-embryo transfer of nutrients such as neutral lipids between 5 and 10 dpc, before the placenta is formed (Bielinska et al., 1999). Like intestinal epithelial cells and hepatocytes, visceral endoderm cells secrete lipoproteins to the epiblast, an embryonic layer (Farese et al., 1996). Thus, we examined lipid distribution in these embryos. Indeed, neutral lipids accumulated in visceral endoderm cells in Vmp1gt/gt embryos at 7.5 dpc (Figure 3C), as observed in vmp1-deficient zebrafish intestinal epithelial cells and hepatocytes.
Intestinal epithelial cell-specific loss of Vmp1 in mice causes accumulation of lipids in intestinal epithelial cells
To circumvent the lethality of Vmp1gt/gt mouse embryos and study the role of VMP1 in the intestine, we generated intestinal epithelial cell-specific Vmp1-deficient mice. Mice harboring a Vmp1flox allele were crossed with Villin-Cre transgenic mice expressing Cre recombinase under the control of the Villin promoter (el Marjou et al., 2004) (Figure 3—figure supplement 1B). Vmp1flox/flox;Villin-Cre mice weighed less than Vmp1flox/+;Villin-Cre mice (Figure 3D). Accumulation of p62 was observed in intestinal epithelial cells in 3-month-old Vmp1flox/flox;Villin-Cre mice (Figure 3E). The intestines of 8-month-old Vmp1flox/flox;Villin-Cre mice showed accumulation of neutral lipids within intestinal epithelial cells (Figure 3F), suggesting a conserved function of VMP1 in the intestine. In the serum from Vmp1flox/flox;Villin-Cre mice, the levels of cholesterol and lipoproteins such as high density lipoprotein (HDL) decreased compared to those from Vmp1flox/+;Villin-Cre mice (Figure 3G). These results suggest that VMP1 is critical for homeostasis of neutral lipids and lipoproteins in a whole body.
VMP1 is important for secretion of lipoproteins
Next, we examined the mechanism by which neutral lipids accumulate in VMP1-depleted organisms. Because intestinal epithelial cells, hepatocytes, and visceral endoderm cells are active in the secretion of lipoproteins (Farese et al., 1996; Sirwi and Hussain, 2018), we hypothesized that a block in lipoprotein secretion is the cause of lipid accumulation. To this end, we used the human hepatocellular carcinoma cell line HepG2 because these cells constitutively secrete lipoproteins. In VMP1-silenced HepG2 cells, the amount of triglyceride and cholesterol decreased in culture media (Figure 4A,B). In contrast, the intracellular amounts increased (Figure 4A,B), suggesting that the secretion of neutral lipids depends on VMP1. Chromatographic analysis using different detection methods for neutral lipids also revealed significant reductions in lipoproteins such as very low-density lipoproteins and low-density lipoproteins in the culture media of VMP1-silenced HepG2 cells both under normal and oleic acid-treated conditions, the latter of which stimulates lipoprotein secretion (Figure 4—figure supplement 1A,B). Likewise, knockdown of VMP1 reduced the amount of APOB in culture media (Figure 4C). Paradoxically, it also reduced the amount of intracellular APOB (Figure 4C). This was likely due to enhanced degradation of misfolded APOB by the ubiquitin-proteasome system because treatment with lactacystin, a proteasome inhibitor, restored the amount of intracellular APOB (Figure 4—figure supplement 1C). Nevertheless, the amount of extracellular APOB remained lower in VMP1-silenced cells than that in control cells (Figure 4—figure supplement 1C). These results suggest that VMP1 is critical not only for APOB homeostasis but also for the secretion of lipoproteins.
-
Figure 4—source data 1
- https://doi.org/10.7554/eLife.48834.012
We next investigated whether VMP1 is required for secretion of proteins besides APOB. Secretion of APOE, a component of very low density lipoprotein (VLDL), and APOA-I, a component of HDL, was also impaired in VMP1-silenced HepG2 cells (Figure 4C). Secretion of APOA-I was affected only slightly. In contrast, secretion of albumin, which is transported from the ER to Golgi separately from lipoproteins (Tiwari and Siddiqi, 2012), was not significantly impaired in VMP1-silenced HepG2 cells (Figure 4C). Secretion of collagens, another type of large cargo that requires TANGO1 for secretion (Saito et al., 2009), was not affected by VMP1 deletion because cartilage structures, which are composed of collagens secreted from cartilage cells, was normal in vmp1-/- zebrafish (Figure 4—figure supplement 1D,E). This result is consistent with a previous report that showed the secretion of collagens and model cargo proteins such as VSVG, a glycoprotein of vesicular stomatitis virus, is normal in vmp1/epg-3 mutant Caenorhabditis elegans and VMP1 knockout COS7 cells, respectively (Zhao et al., 2017). Thus, a defect in secretion in VMP1-deficient cells is not general, but rather specific to lipoproteins.
Neutral lipids accumulate in the ER in the absence of VMP1
In intestinal epithelial cells and hepatocytes, neutral lipids are synthesized within the lipid bilayer of the ER membrane and released into the ER lumen for secretion (Demignot et al., 2014; Sundaram and Yao, 2010; Tiwari and Siddiqi, 2012; Yen et al., 2015). In vmp1-/- zebrafish, almost all large lipid-containing structures in intestinal epithelial cells and hepatocytes were surrounded by the ER transmembrane protein Sec61B (Figure 5A). In most cases, Sec61B covered only a part rather than all of the surface of the lipid structures. Also, in Vmp1gt/gt mouse embryos, almost all neutral lipid-containing structures were positive for SEC61B (Figure 5B). In contrast, neutral lipid structures in these tissues in vmp1+/- animals were mostly negative for Sec61B/SEC61B, suggesting that they are present outside the ER, most likely as cytosolic lipid droplets (Figure 5A,B). Thus, neutral lipids abnormally accumulate in the ER in vmp1-deficient zebrafish and mouse tissues.
-
Figure 5—source data 1
- https://doi.org/10.7554/eLife.48834.014
VMP1 is important for the release of lipoproteins from the ER membrane
We further narrowed down the step defective in VMP1-deficient cells. Neutral lipids accumulating within lipid bilayers of the ER are released into the ER lumen to form lipoproteins together with APOB (Sirwi and Hussain, 2018). In vmp1-/- zebrafish, most of the lipid-containing structures were positive for ApoB (Figure 5C). In addition, the lipid structures were mostly positive for APOB in Vmp1gt/gt mouse embryos (Figure 5D). In agreement with SEC61B staining data, most lipid structures in these tissues in vmp1+/- animals were ApoB/APOB-negative (Figure 5C,D). These results suggest that lipoproteins or lipoprotein-related structures are formed and accumulate in VMP1-deficient cells.
In wild-type HepG2 cells, neutral lipid structures were mostly positive for adipose differentiation-related protein (ADRP, also known as perilipin 2), a marker for cytosolic lipid droplets, but negative for APOB irrespective of oleic acid treatment that increased the number of lipid-containing structures (Figure 6A–C), suggesting that these are lipid droplets rather than lipoproteins. In contrast, as shown in zebrafish and mice (Figure 5), large lipid structures accumulated in VMP1-silenced HepG2 cells (Figure 6A) and most of them were APOB positive (Figure 6C). Some of them were positive for both APOB and ADRP, where APOB and ADRP were distributed into distinct regions (Figure 6D). They should represent structures stuck within the ER lipid bilayers facing both the cytosol and the ER lumen, rather than those released into the ER lumen (Figure 6D). APOE, but not APOA-I, colocalized with APOB on the lipid structures in VMP1-silenced HepG2 cells (Figure 6E,F), suggesting that the defective secretion of APOB and APOE (Figure 4C) is at least partly caused by trapping in the lipid structures.
-
Figure 6—source data 1
- https://doi.org/10.7554/eLife.48834.020
Similar crescent-shaped accumulations of APOB and ADRP around lipids trapped within the ER membranes were also observed in human hepatoma cell line Huh7 cells treated with proteasome inhibitors (Ohsaki et al., 2008). In VMP1-silenced HepG2 cells, however, proteasome activity was not suppressed (Figure 6—figure supplement 1A). In addition, treatment of wild-type HepG2 cells with proteasome inhibitors (MG132 or lactacystin) did not induce crescent-shaped accumulations of APOB and ADRP (Figure 6—figure supplement 2A). These results are somehow different from those in the previous report (Ohsaki et al., 2008), probably because of a difference in cell types or culture conditions. The crescent-shaped accumulations of APOB and ADRP was also observed by treatment with docosahexaenoic acid or cyclosporin A (Ohsaki et al., 2008), which induce APOB proteolysis by unknown molecular mechanisms (Fisher et al., 2001; Kaptein et al., 1994), suggesting the possible involvement of APOB proteolysis in the formation of these structures. APOB was degraded by induction of ER stress or depletion of MTTP (Ota et al., 2008; Sirwi and Hussain, 2018). However, neither ER stress (Figure 6—figure supplement 1B) or reduced MTTP protein level (Figure 6—figure supplement 1C) was observed in VMP1-silenced HepG2 cells. Treatment of wild-type HepG2 cells with ER stress inducers (tunicamycin or thapsigargin) (Figure 6—figure supplement 2B) or an MTTP inhibitor (CP-346086) (Figure 6—figure supplement 2C) did not induce the crescent-shaped accumulations of APOB and ADRP. Furthermore, the crescent-shaped accumulations of APOB and ADRP were not observed in HepG2 cells deficient for FITM2 (Figure 6—figure supplement 2D,E), a factor required for budding of lipid droplets from the ER membrane to the cytosol, but not for lipoprotein secretion (Choudhary et al., 2015; Goh et al., 2015; Kadereit et al., 2008). Taken together, these results suggest that the crescent-shaped accumulations of APOB and ADRP in VMP1-silenced HepG2 cells is not due to proteasome inhibition, ER stress, MTTP suppression, or defective budding of lipid droplets from the ER membrane.
Electron microscopy of intestinal epithelial cells (Figure 7A) and hepatocytes (Figure 7B) of vmp1-/- zebrafish and VMP1-silenced HepG2 cells (Figure 7C) revealed that the ER membranes with ribosomes on their cytosolic face covered a part of the surface of the lipid structures (Figure 7A–C, black arrowheads) and fused with the lipid structures at both ends (Figure 7A–C, white arrowheads). The space between the ER membrane and the lipid structures should be the ER lumen (Figure 7D). In contrast, lipid accumulation within the ER membrane was not observed in vmp1+/- zebrafish and wild-type HepG2 cells (Figure 7A–C, arrows). In hepatocytes in vmp1+/- zebrafish, there was no membrane on large lipid structures, which should represent cytosolic lipid droplets (Figure 7B). These results suggest that neutral lipids abnormally accumulate within the lipid bilayer of the ER membrane in the absence of VMP1, and VMP1 is important for the release of lipoproteins into the ER lumen (Figure 7D).
Discussion
Based on the findings in this study, we propose that the ER protein VMP1 has a novel nonautophagic function in the release of lipoproteins from the ER membrane into the lumen (Figure 7D). This step is distinct from the exit from the ER; it is generally thought that intraluminal lipoproteins are transported to the Golgi (Figure 7D). Consistently, the phenotype of deletion of VMP1 is different from that of factors required for lipoprotein export from the ER lumen to the Golgi (Figure 7D); deletion of TANGO1, TALI (Santos et al., 2016), cTAGE5 (Wang et al., 2016), or SURF4 (Saegusa et al., 2018) does not cause an accumulation of large lipid-containing structures in human hepatocellular carcinoma cells or epithelial colorectal adenocarcinoma cells. However, as it is technically difficult to definitely demonstrate where each apolipoprotein and neutral lipids accumulate in the ER, we do not exclude the possibility that VMP1 is also important at the step of ER-to-Golgi budding, which is not mutually exclusive.
Our results also suggest that VMP1 is important for the release of lipid droplets from the ER membrane to the cytosol because the number of ADRP-positive lipid droplets decreased in VMP1-silenced HepG2 cells (Figure 6A,C). Thus, VMP1 may regulate a common process shared by the three pathways derived from the ER: autophagy, lipoprotein formation, and lipid droplet formation. One hypothesis is that VMP1 might regulate remodeling of the ER membrane. The release of neural lipid-containing structures from the ER membrane to the ER lumen or the cytosol would require drastic reorganization of the membrane (Figure 7D). In addition, during autophagy, the ER membranes dramatically change their shapes and contact with the autophagic membranes (Hayashi-Nishino et al., 2009; Zhao et al., 2017; Zhao et al., 2018). Without VMP1, expansion of the autophagic membranes is defective (Kishi-Itakura et al., 2014; Morita et al., 2018). In Drosophila S2 cells (Bard et al., 2006) and in Dictyostelium (Calvo-Garrido et al., 2008), but not in Caenorhabditis elegans or in mammalian cells (Zhao et al., 2017), VMP1 was reported to be important for the secretion of some soluble cargos. Therefore, in some organisms, VMP1 may also be involved in the budding process of the ER membrane toward the Golgi, and this is achieved possibly by regulating the shape of the ER membrane. Since the release of lipoproteins and lipid droplets from the ER membrane can be regulated by lipid metabolism such as phospholipid remodeling (Ben M'barek et al., 2017; Wang and Tontonoz, 2019), VMP1 could play a possible role in lipid metabolism. Further investigations, in particular, structural analyses of VMP1 and its functionally related protein TMEM41B (Moretti et al., 2018; Morita et al., 2018; Shoemaker et al., 2019), as well as lipidomic analysis of cells lacking these factors, will reveal their molecular functions in the ER membrane.
In this study, we showed that VMP1 is essential for survival during larval periods and early embryonic periods in zebrafish and mice, respectively. Vmp1-deficient mice died around at 8.5 dpc. This timing of lethality is earlier than that of mice deficient for other core autophagy-related genes such as Rb1cc1, Atg13, and Atg5 (Kuma et al., 2017; Mizushima and Levine, 2010). Considering the early embryonic lethality of ApoB- or Mttp-deficient mice around at 10.5 dpc due to malabsorption of lipids from maternal blood (Farese et al., 1995; Farese et al., 1996; Raabe et al., 1998), one of the causes of the early embryonic lethality of Vmp1-deficient mice is likely the same mechanism. The reduction of body weight (Figure 3D) and levels of serum cholesterol and lipoproteins (Figure 3G) in intestinal epithelial cell-specific Vmp1-deficient mice indicates defects in the absorption of nutrients. In contrast to intestinal epithelial cell-specific Mttp-deficient mice (Iqbal et al., 2013; Xie et al., 2006), intestinal epithelial cell-specific Vmp1-deficient mice showed milder phenotypes in lipoprotein secretion; the level of serum triglyceride did not decrease in intestinal epithelial cell-specific Vmp1-deficient mice (Figure 3G). Thus, although VMP1 is important, it is not absolutely essential for lipoprotein secretion.
Two independent genome-wide association studies in humans identified intronic single-nucleotide polymorphism associations (rs11650106 and rs2645492) in the VMP1 gene with altered levels of circulating LDL (Chu et al., 2012; Hoffmann et al., 2018). Thus, VMP1 may be important for the regulation of the levels of circulating lipoproteins in humans. Further examination of the function of VMP1 in a whole organism would provide new insights into the regulation of lipid homeostasis under physiological as well as disease conditions.
Materials and methods
Zebrafish
RIKEN Wako wild-type strain was obtained from the Zebrafish National Bioresource Project of Japan, raised, and maintained in 14 hr light/10 hr dark conditions at 28.5°C according to established protocols (Kimmel et al., 1995). Vmp1-/- zebrafish were generated using the CRISPR/Cas9 system (Jao et al., 2013) including pT7-gRNA, a gift from Wenbiao Chen (plasmid #46759, Addgene), and Cas9 mRNA (CAS500A-1, System Biosciences). A region within exon 6 of zebrafish vmp1 gene was targeted based on CRISPRscan (Moreno-Mateos et al., 2015) (target sequence was 5’-ccaTTGGTGAGCTGCCTCCATAC-3’, where the protospacer adjacent motif is indicated by lower cases). gRNA was synthesized using a MEGAshortscript T7 transcription kit (AM1354, Thermo Fisher Scientific) and purified using a mirVana miRNA Isolation Kit (AM1560, Thermo Fisher Scientific). Wild-type embryos were microinjected at the one-cell stage with 100 pg of sgRNA and 300 pg of Cas9 mRNA using FemtoJet (Eppendorf) equipped with a Femtotip II injection capillary (Eppendorf). For genotyping of vmp1-/- zebrafish, heteroduplex mobility assay (Ota et al., 2014) was performed using genomic DNA, primers flanking the target site (forward primer, 5'-GCTCATCATTTGTACATGCGTGCGTG-3'; reverse primer, 5’-GCTCCAGCATCTCCTCGAATTCTTC-3’), PrimeSTAR Max DNA polymerase (R045A, TaKaRa Bio Inc), and 10% polyacrylamide gels. Vmp1-/- zebrafish were generated by intercrossing vmp1+/- zebrafish harboring a 7 bp deletion. Rb1cc1-/- and atg5-/- zebrafish were generated using rb1cc1+/- zebrafish harboring a 13 bp deletion in exon 4 and atg5+/- zebrafish harboring a 4 bp insertion in exon 3, respectively. Detailed descriptions of the phenotypes of the rb1cc1-/- and atg5-/- zebrafish will be reported elsewhere. Defects in autophagy in rb1cc1-/- zebrafish have been previously confirmed (Kaizuka et al., 2016).
A survival assay of zebrafish larvae was performed on progeny from intercrosses of vmp1+/- zebrafish in the same nursery environment without food. Dead larvae were collected twice a day and frozen. At 13 dpf, the remaining larvae were sacrificed, and all larvae including dead zebrafish were genotyped. Results are shown as Kaplan-Meier survival curves. The external appearance of zebrafish larvae was observed and imaged by a stereoscopic microscope (SZX10, Olympus).
Mice
The Vmp1gt/gt mouse line was generated using the ES cell line Vmp1_F07 (CSD80081) containing an insertion of a gene trap (gt) cassette in the Vmp1 gene (purchased from the Knockout Mouse Project Repository). ES cells were injected into C57BL/6 blastocysts to obtain chimeric mice, which were crossed with C57BL/6 mice to obtain heterozygous mutant mice. For genotyping of Vmp1gt/gt mice, genomic DNA was isolated from the tail or epiblasts dissected from the conceptus and amplified by PCR using primers (F1, 5'-CCCAAGTCTGCTTTACTGACAGCC-3'; F2, 5'-GGGATCTCATGCTGGAGTTCTTCG-3'; R, 5'-TTACTCAGACAGCCTTTCTCCACCC-3') to detect both 445 bp and 640 bp products for wild-type and gt alleles, respectively. The external appearance of mouse embryos was observed and imaged by a stereoscopic microscope (SZX10, Olympus). Wild-type C57BL/6 mice were obtained from Japan SLC, Inc.
The Vmp1flox mice were purchased from The European Mouse Mutant Archive (EM:05506). The exons 3 and 4 of Vmp1 were flanked by two loxP sequences. Cre-mediated depletion of exons 3 and 4 leads to a frameshift, resulting in a small truncated peptide. The following primers were used to detect wild-type and floxed alleles of Vmp1: 5’-GCTTGCTGTGAATGGTTACC-3’ (forward) and 5’-TCAGATCAGCCTTCTGTAGG-3’ (reverse). The expected sizes are 266 bp and 391 bp, respectively. To generate intestinal epithelial cell-specific Vmp1 knockout mice, Vmp1flox/flox mice were crossed with Villin-Cre mice (Model Animal Research Center of Nanjing University). Mice were maintained under specific pathogen-free conditions in the animal facility at the Institute of Biophysics, Chinese Academy of Sciences, Beijing.
All animal experiments were approved by the Institutional Animal Care and Use Committee of the University of Tokyo (Medical-P17-084) and the Institutional Committee of the Institute of Biophysics, Chinese Academy of Sciences (SYXK2016-35).
Cell culture
Request a detailed protocolHepG2 cells (HB-8065, ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; D6546, Sigma-Aldrich) supplemented with 10% fetal bovine serum (172012, Sigma-Aldrich) and 2 mM L-glutamine (25030–081, Gibco; regular medium) in a 5% CO2 incubator. HepG2 cells were regularly tested and found to be mycoplasma-free by DAPI DNA staining. For neutral lipid staining, cells were cultured with regular medium containing 10% serum and 1 μg/mL BODIPY 558/568 C12 (D3835, Thermo Fisher Scientific) for 1 hr.
RNA interference
Request a detailed protocolStealth RNAi oligonucleotide (Thermo Fisher Scientific) against human VMP1 or FITM2 was used for small interfering RNA (siRNA) experiments. The following sequences were used: human VMP1 siRNA 5'-GCAUCAACAGUAUGUGCAACGUAUA-3' and human FITM2 siRNA 5'- AAACAAGGUGCCAAACACCUUCUGG-3'. For the negative control, siRNA against luciferase (5'-CGCGGUCGGUAAAGUUGUUCCAUUU-3') (Thermo Fisher Scientific) was used. The Stealth RNAi oligonucleotides were transfected into cells using Lipofectamine RNAiMAX (13778–150, Thermo Fisher Scientific) according to the manufacturer’s protocols. After 2 days, the cells were again transfected with the same siRNA and cultured for an additional 3 days before analysis.
Antibodies and reagents
Request a detailed protocolFor immunoblotting, goat polyclonal anti-APOB (600-101-111, Rockland Immunochemicals Inc), rabbit polyclonal anti-VMP1 (PM072, MBL International), anti-Albumin (16475–1-AP, Proteintech), anti-BiP (ab21685, Abcam), mouse monoclonal anti-LC3 (CTB-LC3-2-IC, Cosmo Bio), anti-α-tubulin (T9026, clone DM1A, Sigma-Aldrich), anti-β-actin (A2228, clone AC-74, Sigma-Aldrich), anti-APOA-I (66206–1-Ig, clone 1C9G5, Proteintech), anti-APOE (66830–1-Ig, clone 1B2c9, Proteintech), anti-HERP (7039, clone HT2, Chondrex), anti-MTTP (sc-135994, Santa-Cruz), anti-PDI (ADI-SPA-891, clone 1D3, Enzo Life Sciences), and rabbit monoclonal anti-lactate dehydrogenase (LDH; ab52488, Abcam) antibodies were used as primary antibodies. Anti-goat (305-035-003), anti-mouse (115-035-003), and anti-rabbit (111-035-144) horseradish peroxidase-conjugated immunoglobulin G (IgG; Jackson ImmunoResearch Laboratories) were used as secondary antibodies. For immunostaining, goat polyclonal anti-APOB antibody and rabbit polyclonal anti-APOE, anti-ADRP/perilipin2 (15294–1-AP, Proteintech), and anti-APOA-I (ab64308, Abcam) antibodies were used as primary antibodies for the staining of culture cells. Rabbit polyclonal anti-APOB (ab20737, Abcam), anti-SEC61B (15087–1-AP, Proteintech), and anti-p62 (PM045, MBL International) antibodies were used for the staining of tissues. AlexaFluor 488-conjugated anti-goat IgG (A11055, Thermo Fisher Scientific), AlexaFluor 488-conjugated anti-rabbit IgG (A11008, Thermo Fisher Scientific), AlexaFluor 647-conjugated anti-rabbit IgG (A31573, Thermo Fisher Scientific), and AlexaFluor 647-conjugated anti-mouse IgG (A31571, Thermo Fisher Scientific) were used as secondary antibodies. For staining of the mouse intestine, FITC-conjugated anti-rabbit IgG (111-095-003, Jackson ImmunoResearch Laboratories) was used. Hoechst33342 (H342, Dojindo Molecular Technologies) and 4′,6-diamidino-2-phenylindole (DAPI; D9542, Sigma-Aldrich) was used to stain DNA. LipidTOX Red (H34476, Thermo Fisher Scientific) was used to stain neutral lipids. Bovine serum albumin (BSA)-conjugated oleic acid (25630, Nacalai Tesque) was prepared as previously reported (Velikkakath et al., 2012). Lactacystin (4368 v) was purchased from the Peptide Institute Inc. MG132 (M8699), tunicamycin (T7765), thapsigargin (586005) and CP-346086 (PZ0103) were purchased from Sigma-Aldrich.
Live imaging of zebrafish embryos
Request a detailed protocolZebrafish eggs at the one-cell stage were microinjected with 50 ng/µL of GFP-LC3 mRNA, which was synthesized from pcDNA3-GFP-LC3-RFP-LC3ΔG plasmid (Kaizuka et al., 2016) using a mMESSAGE mMACHINE T7 Transcription Kit (AM1344, Thermo Fisher Scientific) and purified using RNeasy Mini Kit (74104, Qiagen). Embryos were anesthetized with 0.03% tricaine (A5040, Sigma-Aldrich), placed in water on a glass-bottomed dish, and viewed using a confocal microscope (FV1000 IX81; Olympus) with an objective lens (UPLSAPO30XS, Olympus).
Immunohistochemistry
Request a detailed protocolImmunohistochemistry of tissues was performed as described previously (Morishita et al., 2013). In brief, zebrafish larvae and mouse embryos dissected from the conceptus were fixed in 4% paraformaldehyde (PFA) overnight at 4°C, infiltrated with 15% and 30% sucrose in phosphate-buffered saline (PBS) for 4 hr each, and embedded in Tissue-Tek OCT Compound (Sakura Japan Co.). Sections (7 μm) were prepared using a cryostat (CM3050 S, Leica Microsystems) and mounted on slides. For whole-mount staining of mouse embryos, embryos were dissected from the conceptus and fixed with 4% PFA for 15 min at 4°C. Cryosections or mouse embryos were washed with PBS, treated with 0.05% Triton X-100 for 15 min, blocked with 3% BSA in PBS for 30 min, and incubated with primary antibodies for 1 hr, followed by PBS wash and incubation with secondary antibodies for 1 hr. For staining of neutral lipids and nuclear DNA, samples were treated with LipidTOX Red and Hoechst33342 in PBS for 30 min and washed three times with PBS. The coverslips and mouse embryos in a glass-bottomed dish were mounted with SlowFade antifade reagents (S36936, Thermo Fisher Scientific), viewed using a confocal laser microscope (FV1000 IX81, Olympus), and captured with FluoView software (Olympus). The number of punctate structures was determined using FIJI software (ImageJ, National Institutes of Health) (Schindelin et al., 2012).
For p62 and DAPI staining in intestinal epithelial cell-specific Vmp1-deficient mice, sections were deparaffinized in xylene and rehydrated in an ethanol series (100% × 3, 95%, and 75%). Antigen retrieval was performed using microwaves (0.01 M citrate buffer for 10 min). After blocking, sections were incubated with primary antibodies at 4°C overnight. After washing three times in PBS, sections were incubated with fluorescent-labeled secondary antibodies for 1 hr at room temperature. Samples were then counterstained with DAPI and detected under a confocal microscope (LSM 880 Meta plus Zeiss Axiovert zoom, Zeiss).
Oil red O and Nile red staining
Request a detailed protocolWhole-mount oil red O staining was performed according to a previous method (Dai et al., 2015). In brief, zebrafish larvae were maintained in 0.2 mM 1-phenyl-2-thiourea (PTU) to avoid pigmentation from one dpf, fixed with 4% PFA overnight at 4°C, washed twice with PBS, infiltrated with 80% and 100% 1,2-propylene glycol for 30 min each, and stained with 0.5% oil red O (O0625, Sigma-Aldrich) in 100% 1,2-propylene glycol overnight at room temperature. Stained larvae were washed twice with PBS and the background color was faded with 100% and 80% 1,2-propylene glycol for 30 min each, and observed by a stereoscopic microscope (SZX10, Olympus). For oil red O staining of cryosections, zebrafish larvae were fixed in 4% PFA overnight at 4°C, infiltrated with 15% and 30% sucrose for 4 hr each at 4°C, and embedded in Tissue-Tek OCT Compound. Sections (6 μm) were mounted on slides and stained with oil red O in 60% isopropanol for 15 min at 37°C, followed by a 60% isopropanol wash, staining with hematoxylin for 3 min, and a wash with water. Slides were visualized using a microscope (BX51, Olympus) equipped with a digital camera (DP70, Olympus).
For Nile red staining in intestinal epithelial cell-specific Vmp1-deficient mice fed ad libitum, frozen tissues were embedded and cryostat sectioned. Sections were washed three times with PBS and then stained with Nile red (1:1000, N1142, Thermo Fisher Scientific) for 15 min at room temperature. Coverslips were mounted with DAPI and examined under a confocal microscope (LSM 880 Meta plus Zeiss Axiovert zoom, Zeiss).
Alcian blue staining
Request a detailed protocolWhole-mount Alcian blue staining to visualize the cartilage was performed according to the previous methods (Walker and Kimmel, 2007). In brief, zebrafish larvae were maintained in 0.2 mM PTU from one dpf, fixed with 4% PFA overnight at 4°C, dehydrated with 50% ethanol for 10 min, stained with acid-free stain solution (0.02% Alcian blue (A5268, Sigma-Aldrich), 60 mM MgCl2, 70% ethanol) at room temperature overnight, cleared with 50% glycerol and 0.25% KOH at room temperature for 2 hr, dipped into 50% glycerol and 0.1% KOH, and viewed and photographed with a stereoscopic microscope (SZX10, Olympus).
Immunocytochemistry
Request a detailed protocolCells grown on coverslips (S2441, Matsunami) were washed with PBS and fixed with 4% PFA for 15 min at room temperature. Fixed cells were permeabilized with 0.1% Triton X-100 (35501–15, Nacalai Tesque) in PBS for 5 min and blocked with 3% BSA in PBS and incubated with specific antibodies for 1 hr. After washing with PBS, cells were incubated with Alexa Fluor 488- or 647-conjugated secondary antibodies for 1 hr. The coverslips were viewed using a confocal laser microscope (FV1000 IX81, Olympus) with a 100 × oil immersion objective lens (Olympus) and captured with FluoView software (Olympus). For the final output, the images were processed using Photoshop CS6 (Adobe). ImageJ software was used for quantification of the number and total pixel area of lipid-containing structures and the number of APOB- or ADRP-positive neutral lipid particles.
Electron microscopy
Request a detailed protocolZebrafish larvae were dissected at the abdomen under anesthesia with 0.03% tricaine, and fixed with 2% glutaraldehyde and 2% PFA in 0.1 M sodium cacodylate buffer (0.1% calcium chloride) overnight. For HepG2 cells, cells were cultured on a poly-L-lysine coated cell tight C-2 cell disk (MS-0113K, Sumitomo Bakelite) and fixed in 2.5% glutaraldehyde (G015, TAAB) in 0.1 M phosphate buffer (pH 7.4) for 2 hr. Tissues and cells were then post-fixed with 1.0% osmium tetroxide in 0.1 M phosphate buffer for 2 hr, dehydrated, and embedded in Epon 812 according to a standard procedure. Ultrathin sections were stained with uranyl acetate and lead citrate and observed using an H-7100 electron microscope (Hitachi).
Immunoblotting
Request a detailed protocolZebrafish embryos or HepG2 cells were lysed with lysis buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 1 mM phenylmethanesulfonyl fluoride, and complete EDTA-free protease inhibitor cocktail [19543200, Roche]). After centrifugation at 15,000 × g for 20 min, the supernatants were collected, and the protein concentrations were adjusted using the bicinchoninic acid method (23228, Thermo Fisher Scientific). The lysates were solubilized with immunoblot sample buffer (46.7 mM Tris-HCl [pH 6.8], 5% glycerol, 1.67% sodium dodecyl sulfate [SDS], 1.55% dithiothreitol, and 0.003% bromophenol blue). The immunoblot samples were separated by SDS-polyacrylamide gel electrophoresis, transferred to an Immobilon-P polyvinylidene difluoride membrane (IPVH00010, Millipore), and blotted with primary and secondary antibodies. Each protein signal was detected with Super-Signal West Pico Chemiluminescent substrate (1856136, Thermo Fisher Scientific) or Immobilon Western Chemiluminescent HRP substrate (WBKLS0500, Millipore). Signal intensities were captured using FUSION SOLO7S (Vilber-Lourmat). The images were processed using Photoshop CS6 (Adobe).
Lipoprotein secretion assay
Request a detailed protocolHepG2 cells were treated with siRNA twice as described above and cultured in serum-free DMEM supplemented with 200 nM oleic acids-BSA for 24 hr in 12-well plates. The cells were washed with PBS and re-cultured in serum-free DMEM before analysis. The culture medium was centrifuged at 5000 x g for 3 min to remove any cells or cellular debris. The culture medium was then precipitated with 10% trichloroacetic acid (TCA), and the pellets were washed with ice-cold acetone twice and dissolved in immunoblot sample buffer. The cells were washed with PBS and lysed as described above. Samples (approximately 7% or 14% vol of total precipitated media or cell lysates, respectively) were subjected to immunoblot analysis. ImageJ software was used for densitometric quantification.
For chromatography analysis, siRNA-treated HepG2 cells (approximately 1.5 × 106 cells) were cultured in 0.1% BSA containing DMEM with or without oleic acids-BSA for 48 hr before analysis. Lipoproteins in culture medium were analyzed using the gel permeation high performance liquid chromatography (Skylight Biotech Inc) system as previously described (Okazaki and Yamashita, 2016). Briefly, lipoproteins in culture medium were separated with tandemly connected Skylight PakLP1-AA gel permeation columns (Skylight Biotech Inc; 300 mm × 4.6 mm I.D.). The column effluent was then equally split into two lines by a micro splitter, and each effluent was allowed to react at 37°C with the cholesterol and triglyceride reagents. Absorbance at 550 nm was continuously monitored after each enzymatic reaction in two reactor coils (PTFE; 25 m × 0.18 mm I.D.).
Triglyceride and cholesterol quantification
Request a detailed protocolSix pairs of 18-month-old Vmp1flox/+;Villin-Cre mice and Vmp1flox/flox;Villin-Cre mice fed ad libitum were examined. Blood was allowed to stand for 2 hr and centrifuged at 1600 g for 15 min for collecting the serum. Serum samples were then measured by OLYMPUS AU480 automatic biochemical analyzer.
HepG2 cells were treated with siRNA twice as described above. HepG2 cells (approximately 1 × 105 cells) were cultured in serum-free medium for 24 hr before analysis. For total lipid extraction from culture medium, the Bligh and Dyer method was performed. Both extra- and intra-cellular cholesterol and triglyceride levels were measured using quantitation kits (K603-100 and K622-100, respectively, Biovision Inc) according to the manufacturer’s protocols.
Measurement of proteasome activity
Request a detailed protocolProteasomal activity was measured with the Proteasome-Glo kit (G8660, Promega). HepG2 cells were treated with siRNA twice as described above and plated equally (approximately 1 × 104 cells per well) in 96-well white-walled plates. The cells were cultured with or without 5 μM lactacystin for 2 hr before measurement. Proteasome-Glo Cell-Based Reagent was prepared as per manufacturer’s protocol and an equal volume was added to each well. The plate was mixed for 2 min using TAITEC E-36 micromixer and then incubated at room temperature for 10 min. Luminescence was measured by a microplate leader EnSpire (2300–00J, PerkinElmer).
Quantitative real-time PCR
Request a detailed protocolTotal RNA was extracted from HepG2 cells using ISOGEN (319–90211, Nippon Gene) and reverse-transcribed using ReverTraAce (FSQ-201, TOYOBO) according to the manufacturer’s instructions. PCR was performed by a Thermal Cycler Dice TP800 (TaKaRa Bio Inc) in triplicate using TB Green Premix Ex Taq II (RR820, TaKaRa Bio Inc). The expression level of FITM2 was normalized to that of ACTB. Primers used are listed as follows: FITM2, 5′-AAAGGAACACCAGAGCAAGC-3′ and 5′-CCTCATGCAGCACAGACATC-3′; ACTB, 5′-ATTGCCGACAGGATGCAGAA-3′ and 5′-ACATCTGCTGGAAGGTGGACAG-3′.
Statistical analysis
Request a detailed protocolStudent t-test or Mann-Whitney U-test were performed to compare two groups. All data are presented as the mean ± standard error of the mean unless otherwise stated. Statistical analysis was performed using R software (R Core Team) or GraphPad Prism seven software (GraphPad software).
Data availability
All data generated or analysed during this study are included in the manuscript files. Source data files have been provided for Figures (1, 3, 4, 5, and 6), Figure 4—figure supplement 1, Figure 6—figure supplement 1, and Figure 6—figure supplement 2.
References
-
Distinct roles for visceral endoderm during embryonic mouse developmentThe International Journal of Developmental Biology 43:183–205.
-
A conserved family of proteins facilitates nascent lipid droplet budding from the ERThe Journal of Cell Biology 211:261–271.https://doi.org/10.1083/jcb.201505067
-
APOLIPOPROTEIN B: mrna editing, lipoprotein assembly, and presecretory degradationAnnual Review of Nutrition 20:169–193.https://doi.org/10.1146/annurev.nutr.20.1.169
-
Cloning and expression of the rat vacuole membrane protein 1 (VMP1), a new gene activated in pancreas with acute pancreatitis, which promotes vacuole formationBiochemical and Biophysical Research Communications 290:641–649.https://doi.org/10.1006/bbrc.2001.6244
-
A novel function for apolipoprotein B: lipoprotein synthesis in the yolk sac is critical for maternal-fetal lipid transport in miceJournal of Lipid Research 37:347–360.
-
The triple threat to nascent apolipoprotein B. evidence for multiple, distinct degradative pathwaysThe Journal of Biological Chemistry 276:27855–27863.https://doi.org/10.1074/jbc.M008885200
-
Postnatal deletion of fat Storage-inducing transmembrane protein 2 (FIT2/FITM2) Causes lethal enteropathyJournal of Biological Chemistry 290:25686–25699.https://doi.org/10.1074/jbc.M115.676700
-
FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cellsThe Journal of Cell Biology 181:497–510.https://doi.org/10.1083/jcb.200712064
-
A subdomain of the endoplasmic reticulum forms a cradle for autophagosome formationNature Cell Biology 11:1433–1437.https://doi.org/10.1038/ncb1991
-
Zebrafish: gaining popularity in lipid researchBiochemical Journal 429:235–242.https://doi.org/10.1042/BJ20100293
-
Lipid absorption defects in intestine-specific microsomal triglyceride transfer protein and ATP-binding cassette transporter A1-deficient miceJournal of Biological Chemistry 288:30432–30444.https://doi.org/10.1074/jbc.M113.501247
-
The Beclin 1 network regulates autophagy and apoptosisCell Death & Differentiation 18:571–580.https://doi.org/10.1038/cdd.2010.191
-
Cotranslational inhibition of apoB-100 synthesis by cyclosporin A in the human hepatoma cell line HepG2Arteriosclerosis and Thrombosis: A Journal of Vascular Biology 14:780–789.https://doi.org/10.1161/01.ATV.14.5.780
-
Stages of embryonic development of the zebrafishDevelopmental Dynamics 203:253–310.https://doi.org/10.1002/aja.1002030302
-
Ultrastructural analysis of autophagosome organization using mammalian autophagy-deficient cellsJournal of Cell Science 127:4089–4102.https://doi.org/10.1242/jcs.156034
-
Digesting the expanding mechanisms of autophagyTrends in Cell Biology 26:624–635.https://doi.org/10.1016/j.tcb.2016.03.006
-
Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cellsThe Journal of Cell Biology 152:657–668.https://doi.org/10.1083/jcb.152.4.657
-
The role of atg proteins in autophagosome formationAnnual Review of Cell and Developmental Biology 27:107–132.https://doi.org/10.1146/annurev-cellbio-092910-154005
-
Autophagy in mammalian development and differentiationNature Cell Biology 12:823–830.https://doi.org/10.1038/ncb0910-823
-
Deletion of autophagy-related 5 (Atg5) and Pik3c3 genes in the Lens causes cataract independent of programmed organelle degradationThe Journal of Biological Chemistry 288:11436–11447.https://doi.org/10.1074/jbc.M112.437103
-
Genome-wide CRISPR screen identifies TMEM41B as a gene required for autophagosome formationThe Journal of Cell Biology 217:3817–3828.https://doi.org/10.1083/jcb.201804132
-
Dynamics and diversity in autophagy mechanisms: lessons from yeastNature Reviews Molecular Cell Biology 10:458–467.https://doi.org/10.1038/nrm2708
-
Lipid droplets are arrested in the ER membrane by tight binding of lipidated apolipoprotein B-100Journal of Cell Science 121:2415–2422.https://doi.org/10.1242/jcs.025452
-
Inhibition of apolipoprotein B100 secretion by lipid-induced hepatic endoplasmic reticulum stress in rodentsJournal of Clinical Investigation 118:316–332.https://doi.org/10.1172/JCI32752
-
The pancreatitis-induced vacuole membrane protein 1 triggers autophagy in mammalian cellsJournal of Biological Chemistry 282:37124–37133.https://doi.org/10.1074/jbc.M706956200
-
SFT-4/Surf4 control ER export of soluble cargo proteins and participate in ER exit site organizationThe Journal of Cell Biology 217:2073–2085.https://doi.org/10.1083/jcb.201708115
-
TANGO1 and Mia2/cTAGE5 (TALI) cooperate to export bulky pre-chylomicrons/VLDLs from the endoplasmic reticulumThe Journal of Cell Biology 213:343–354.https://doi.org/10.1083/jcb.201603072
-
Fiji: an open-source platform for biological-image analysisNature Methods 9:676–682.https://doi.org/10.1038/nmeth.2019
-
Lipid transfer proteins in the assembly of apoB-containing lipoproteinsJournal of Lipid Research 59:1094–1102.https://doi.org/10.1194/jlr.R083451
-
Membrane trafficking in autophagyInternational Review of Cell and Molecular Biology 336:1–92.https://doi.org/10.1016/bs.ircmb.2017.07.001
-
Intracellular trafficking and secretion of VLDLArteriosclerosis, Thrombosis, and Vascular Biology 32:1079–1086.https://doi.org/10.1161/ATVBAHA.111.241471
-
A two-color acid-free cartilage and bone stain for zebrafish larvaeBiotechnic & Histochemistry 82:23–28.https://doi.org/10.1080/10520290701333558
-
Phospholipid remodeling in physiology and diseaseAnnual Review of Physiology 81:165–188.https://doi.org/10.1146/annurev-physiol-020518-114444
-
Compensatory increase in hepatic lipogenesis in mice with conditional intestine-specific mttp deficiencyJournal of Biological Chemistry 281:4075–4086.https://doi.org/10.1074/jbc.M510622200
-
Intestinal triacylglycerol synthesis in fat absorption and systemic energy metabolismJournal of Lipid Research 56:489–501.https://doi.org/10.1194/jlr.R052902
Article and author information
Author details
Funding
Japan Science and Technology Agency (JPMJER1702)
- Noboru Mizushima
Japan Society for the Promotion of Science (25111005)
- Noboru Mizushima
National Natural Science Foundation of China (31671430)
- Yan G. Zhao
Japan Society for the Promotion of Science (18K14694)
- Hideaki Morishita
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Acknowledgements
We thank Hong Zhang for continuous encouragement and support, Kota Saito for help with the secretion assays, Nozomi Sato and Tomoya Eguchi for care of the zebrafish and mice, Akira Ohtsuka and Akiko Kuma for help in establishing the mouse genotyping protocols, Keiko Igarashi for help with the histological assays, Chieko Saito for help with electron microscopy, and Yuki Ohsaki, Toyoshi Fujimoto, Takeshi Sugawara, and Hayashi Yamamoto for the helpful discussions.
Ethics
Animal experimentation: All animal experiments were approved by the Institutional Animal Care and Use Committee of the University of Tokyo (Medical-P17-084) and the Institutional Committee of the Institute of Biophysics, Chinese Academy of Sciences (SYXK2016-35).
Copyright
© 2019, Morishita et al.
This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 4,120
- views
-
- 595
- downloads
-
- 49
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Download links
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Further reading
-
- Cell Biology
The β-catenin-dependent canonical Wnt signaling is pivotal in organ development, tissue homeostasis, and cancer. Here, we identified an upstream enhancer of Ctnnb1 – the coding gene for β-catenin, named ieCtnnb1 (intestinal enhancer of Ctnnb1), which is crucial for intestinal homeostasis. ieCtnnb1 is predominantly active in the base of small intestinal crypts and throughout the epithelia of large intestine. Knockout of ieCtnnb1 led to a reduction in Ctnnb1 transcription, compromising the canonical Wnt signaling in intestinal crypts. Single-cell sequencing revealed that ieCtnnb1 knockout altered epithelial compositions and potentially compromised functions of small intestinal crypts. While deletion of ieCtnnb1 hampered epithelial turnovers in physiologic conditions, it prevented occurrence and progression of Wnt/β-catenin-driven colorectal cancers. Human ieCTNNB1 drove reporter gene expression in a pattern highly similar to mouse ieCtnnb1. ieCTNNB1 contains a single-nucleotide polymorphism associated with CTNNB1 expression levels in human gastrointestinal epithelia. The enhancer activity of ieCTNNB1 in colorectal cancer tissues was stronger than that in adjacent normal tissues. HNF4α and phosphorylated CREB1 were identified as key trans-factors binding to ieCTNNB1 and regulating CTNNB1 transcription. Together, these findings unveil an enhancer-dependent mechanism controlling the dosage of Wnt signaling and homeostasis in intestinal epithelia.
-
- Cell Biology
- Stem Cells and Regenerative Medicine
Our understanding of the transitions of human embryonic stem cells between distinct stages of pluripotency relies predominantly on regulation by transcriptional and epigenetic programs with limited insight on the role of established morphological changes. We report remodeling of the actin cytoskeleton of human embryonic stem cells (hESCs) as they transition from primed to naïve pluripotency which includes assembly of a ring of contractile actin filaments encapsulating colonies of naïve hESCs. Activity of the Arp2/3 complex is required for the actin ring, to establish uniform cell mechanics within naïve colonies, promote nuclear translocation of the Hippo pathway effectors YAP and TAZ, and effective transition to naïve pluripotency. RNA-sequencing analysis confirms that Arp2/3 complex activity regulates Hippo signaling in hESCs, and impaired naïve pluripotency with inhibited Arp2/3 complex activity is rescued by expressing a constitutively active, nuclear-localized YAP-S127A. Moreover, expression of YAP-S127A partially restores the actin filament fence with Arp2/3 complex inhibition, suggesting that actin filament remodeling is both upstream and downstream of YAP activity. These new findings on the cell biology of hESCs reveal a mechanism for cytoskeletal dynamics coordinating cell mechanics to regulate gene expression and facilitate transitions between pluripotency states.