Intestinal infection regulates behavior and learning via neuroendocrine signaling

Acceptance summary:

The manuscript challenges the current view that induced expression of daf-7 in ASJ sensory neurons has the major role in transmitting the pathogen information for generation of the pathogen avoidance behavior, because the authors show that expression of daf-7 in ASJ is not correlated with pathogen avoidance, but they instead show clearly that intestinal bloating is the major cause. Upon revision, authors addressed all major concerns that the reviewers raised. We are therefore glad to accept the manuscript for publication in eLife.

Decision letter after peer review:

Thank you for submitting your article "Intestinal infection regulates behavior and learning via neuroendocrine signaling" for consideration by eLife. Your article has been reviewed by three peer reviewers, and the evaluation has been overseen by a Reviewing Editor and K VijayRaghavan as the Senior Editor. The following individuals involved in review of your submission have agreed to reveal their identity: Javier Irazoqui (Reviewer #1).

The reviewers have discussed the reviews with one another and the Reviewing Editor has drafted this decision to help you prepare a revised submission.

All three reviewers appreciate the importance of the work, especially because it challenges the prevailing view of the major role of daf-7 in ASJ in transmitting the pathogen information for generation of the pathgen avoidance behavior. However, they also agree that some of the authors' assertion needs to be substantiated further. We therefore invite revision of the manuscript, and as a consensus of the reviewers, we request the following to be addressed in revision.

Essential revisions:

1) The authors seem to claim that the pathogen avoidance behavior is driven by aerotaxis (at least in the present manuscript it sounds so). However, is it really aerotaxis which causes preference of E. coli over pathogenic bacteria after pathogenic exposure? The authors' claim is mainly based on the fact that lawn of P. aeruginosa has lower oxygen level than that of E. coli, and the requirement of npr-1, ocr-2 and osm-9 for avoidance and suppression by egl-9. However, these pathways regulate other functions as well. We request authors either test whether dead bacteria cannot cause the avoidance behavior, or perform behavioral assay that directly tests aerotaxis. Another option is to weaken the claim that it is aerotaxis.

2) Genetic requirements were assessed by RNAi for nol-6, aex-5 and egl-8. Because RNAi does not always generate specific and complete loss of the targeted gene, and because mutants are available for all these genes, the results need to be backed up by using respective mutants.

3) The following point is not an absolute requirement, but it would be nice to be added: this study intriguingly shows that increased ASJ-expression of daf-7 has no correlation with lawn avoiding. Since this finding will be very important for the field, it will be useful to provide a more complete analysis on daf-7 by including ASI-expression of daf-7, because Meisel et al. shows that daf-7 in ASI also rescues the lawn avoidance defects of daf-7 mutant and the expression of daf-7 in ASI also increases by exposure to Pseudomonas.

Reviewer #1:

In this very interesting paper, Singh and Aballay tackle a central question in innate immunity in general and in C. elegans innate immunity in particular: how is infection detected and host defense mechanisms engaged in vivo? The paradigm in this manuscript is C. elegans aversive learning, in which behavioral innate immunity is activated during infection so that the animal can escape an unfavorable (pathogenic) environment. The assays are very simple: in one case (lawn assay), the animals are exposed to stimuli (chemical, bacterial) on lawns of bacteria, and then their preference to stay on the lawn (and, thus, feed) or avoid it (and, thus, escape infection) is measured over time. In the other case (choice assay), animals reared on non-pathogenic E. coli are given the choice to continue eating E. coli or to feed on the "tastier" Pseudomonas aeruginosa – which is pathogenic.

First, the investigators dispel the notion, put forward in Meisel et al. (2014) in Cell, that sensory-neuron-mediated detection of phenazine-1-carboxamide is the trigger of aversive learning and pathogen avoidance through the induction of daf-7 expression in ASJ neurons. This result is extremely important to the field, as many researchers have expressed their doubts about such a simplistic model, and is consistent with prior research from the Ausubel lab that showed that phenazine mutants of P. aeruginosa are just as virulent as wild type (replicated in this study).

Then, the investigators set out to determine what really triggers aversion during infection. First, they find that "older" solid cultures of P. aeruginosa are more virulent than "younger" ones. They establish that virulence correlates with intestinal distention, or "bloating". Drawing on their seminal work published earlier this year in Dev Cell, they find that older more pathogenic Pseudomonas induce gut-derived neuropeptides more strongly than younger ones, and that intestinal distention is required for aversive learning in the lawn assay. Then, they show that avirulent mutants of Pseudomonas, identified in a whole genome screen by the Ausubel lab, are defective in inducing aversive learning by the same assay. The nail in the phenazine/daf-7 coffin is presented in Figure 4F,G, where they show a complete lack of correlation between daf-7 ASJ expression and aversive learning, but almost perfect correlation with Pseudomonas virulence. After establishing that, they turn to preference. What makes E. coli less "tasty" than Pseudomonas, despite the virulence? Why does this choice invert after about 12 hours? To answer the first question, they examine aerotaxis. Consistent with the idea (and previous research) that Pseudomonas lawns exhibit lower oxygen tension (preferred by wild type C. elegans) than E. coli ones, they find induction of a HIF-1 target gene only in animals feeding on Pseudomonas, not E. coli. Then, they examine mutants. daf-7 mutant exhibit no choice; npr-1 mutants invert their choice more slowly than wild type. The double mutants show additive effects. Importantly, they also show that distention-resistant nol-6 RNAi worms also do not invert their choice – they never learn. Conversely, ocr-2 and osm-9 mutants, which prefer higher oxygen tensions, switch their preference to E. coli and learn to avoid Pseudomonas lawns faster than wild type; this is suppressed by loss of egl-9, a negative regulator of HIF-1. The implication, which is not substantiated or tested, is that HIF-1 activation and thus target gene transcription is epistatic to the loss of aerotaxis behavior phenotype in ocr-2 or osm-9 mutants.

In summary, this important study corrects the record on an important subject, and identifies the genetics in host and pathogen that are involved in aversive learning and food choice during infection.

Reviewer #2:

Singh and Aballay examine the mechanisms that regulate the avoidance of pathogenic P. aeruginosa bacteria in C. elegans.

They first test the role of several phenazines in eliciting avoidance and increasing daf-7 expression in ASJ. They report that only one type of phenazine increases daf-7 expression in ASJ, but it does not generate avoidance when put on E. coli. They also find that another type of phenazine repels worms although it does not increase ASJ expression of daf-7 and mutants lacking phenazines repel worms similarly as wild type. These results show that phenazines are not required for the repulsion of the worms.

Next, they examine whether the growth conditions regulate lawn repulsion by using 72 hour lawns and 12 hour lawns. They found that 72 hour lawns that strongly repel worms cause intestine bloating but 12 hour lawns that repel worms less do not cause bloating. Meanwhile, the 72 hour lawns, but not the 12 hour lawns, also regulate transcription similarly as intestine bloating and decreasing bloating by nol-6 RNAi delays avoidance. Meanwhile, they show that enhancing intestine bloating (aex-5, egl-8 RNAi) enhances repulsion. These results demonstrate a strong link between intestine bloating and lawn avoidance.

Finally, they find that several P. aeruginosa mutants with weaker virulence show decreased repulsion to the worms and mutating aerotaxis gene oms-9 and ocr-2, alters the switch of preference from pathogenic P. aeruginosa to E. coli.

The findings in this study demonstrate a strong link between intestine bloating and lawn avoidance and provide a new mechanism for the worms to avoid infectious bacteria. Several questions need to be addressed.

1) The results and the analyses using RNAi should be supported by genetic experiments.

2) Previous studies show that exposure to the pathogenic P. aeruginosa bacteria increases expression of daf-7 in both ASJ and ASI and that daf-7 expression in either neuron rescues the lawn leaving phenotype in daf-7. While this work tests the association between ASJ expression of daf-7 and lawn leaving behavior, it will be helpful in furthering the understanding of daf-7 function by examining ASI expression of daf-7.

3) The manuscript states that the findings on ASJ expression of daf-7 argue against the message provided by a previous study (Meisel et al., 2014). This is confusing, because Meisel et al. does not seem to propose that increased expression of daf-7 in ASJ, induced by pathogenic P. aeruginosa or phenazine-1-carboxamide and pyochelin, elicits avoidance. Rather, they propose that it is one of sensory responses to the bacteria (for example, they conclude that "Our findings demonstrate how specific bacteria can exert effects on host behavior and physiology and point to how secondary metabolites may serve as environmental cues that contribute to pathogen discrimination and avoidance."). It is important to clarify these issues for the readers to better understand the complexity of the question.

Reviewer #3

In "Intestinal infection regulates behavior and learning via neuroendocrine signaling," the authors show that feedback from the intestine modulates the worm behavioral response to infection with Pseudomonas aeruginosa – namely pathogen avoidance. The response requires daf-7 and npr-1; loss of either of these genes reduces pathogen avoidance and loss of both completely blocks the process. It had previously been thought that daf-7 expression in the ASJ neurons might drive this process because a daf-7p::GFP reporter construct is activated in these neurons upon exposure to P. aeruginosa. However, the authors show that the behavior is not correlated with this expression. In one example, 12 hour and 72 hour lawns of P. aeruginosa cause equal amounts of daf-7p::GFP expression, but elicit different levels of lawn avoidance. In a second example, worms are exposed to mutants of P. aeruginosa that change how avidly they avoid the lawns, but the degree of avoidance is not correlated with the levels of induced ASJ daf-7p::GFP expression. Rather, the authors argue that the level of intestinal bloating caused by the P. aeruginosa infection drives the pathogen avoidance response. Several examples of correlation are given.

1) The phenazine 1-hydroxyphenazine is the only one of four tested that causes both bloating and avoidance.

2) A 72 hour lawn causes more rapid bloating and more rapid avoidance.

3) The degree of bloating caused by different P. aeruginosa mutants is correlated with the degree of avoidance.

4) C. elegans mutants that have more bloating have more avoidance, and those that have less bloating have less avoidance. The data in total supports in a correlative manner the authors' postulate that the changes in the intestine (bloating) causes the ensuing pathogen avoidance behavior.

The authors then argue that it is aerotaxis behavior via neuroendocrine signaling that drives the learned pathogen avoidance behavior. Worms like bacterial lawns with less oxygen and P. aeruginosa lawns are known to have less oxygen. However, following intestinal bloating and activation of the NPR-1/DAF-7 neuroendocrine pathway on P. aeruginosa, the animals come to prefer the higher oxygen lawns of E. coli. The argument is supported by the fact that animals mutant in daf-7 and/or npr-1 cannot learn to avoid P. aeruginosa lawns.

Overall, this is a very interesting study and it will be of broad interest to the worm community. However, I am concerned about the heavy dependence on correlative results on which the main conclusions rest.

1) Are oxygen levels really the signal the worms learn to recognize as a sign of a pathogenic lawn or is it a correlation? Is there a way to equalize the oxygen levels between the pathogenic and non-pathogenic lawns? Then one could see if the worms can still learn to avoid the lawn (and if intestinal bloating still occurs (see point 2)). Alternatively, maybe the animals trained to avoid P. aeruginosa could be tested in an assay designed to test their aerotaxis preferences?

2) The relationship, if any, between low/high oxygen and intestinal bloating is not clear. Does the low oxygen cause the bloating on P. aeruginosa, or could it? Or do the authors think that the high/low oxygen is just how the worms learn to distinguish between a good (E. coli) and bad (P. aeruginosa) lawn?

3) Worms like bacterial lawns with less oxygen and P. aeruginosa lawns are known to have less oxygen. Do the authors think this explains the initial attraction to the P. aeruginosa lawn?