UMAP plots of expression in the neural restricted subset of scRNA-Seq for (A) l1cam, (G) uchl1, (M) nsg1, and (s) ass1. UMAP plots of expression in the superset scRNA-Seq dataset for (B) l1cam, (H) uchl1, (N) nsg1, and (T) ass1. Note that the topology of neural cell clusters is slightly different when subset and reclustered to give high neural cluster resolution (A,G,M,S) than when neural cells are analyzed together with all other cell types (E,K, Q, W), but the same cells and expression data are included in both cases. Dot plots representing average expression of (C) l1cam, (I) uchl1, (O) nsg1, and (U) ass1 per cluster and timepoint. In situ hybridization at 0hpa, 24hpa, and 72hpa for (D–F) l1cam, (J–L) uchl1, (P–R) nsg1, and (V–X) ass1. (Y–Z’) Representative images of 24hpa (Y/Y’) and 72hpa (Z/Z’) regenerated tails from a pax6:GFP transgenic tadpole stained for DAPI (cyan) and mitotic cells with phospho-histone3 (magenta). The white arrows indicate regeneration plane. (Y’) and (Z’) are enlarged images of the boxed areas in (Y) and (Z). (AA) Boxplot representing the number of cells per regenerated spinal cord area (mm2) at 24hpa and 72hpa. Statistics represent t-test performed between the two timepoints. Abbreviations: Neural Progenitor Cell (NPC), Differentiating Neuron (Diff), Interneuron (IN), Vulnerable Motor Neuron (VMN), Dopaminergic Neuron (DN), Motor Neuron Leptin+ (MNL), Motor Neuron (MN)).