TorsinB overexpression prevents abnormal twisting in DYT1 dystonia mouse models

  1. Jay Li
  2. Chun-Chi Liang
  3. Samuel S Pappas  Is a corresponding author
  4. William T Dauer  Is a corresponding author
  1. University of Michigan, United States
  2. University of Texas Southwestern, United States

Abstract

Genetic redundancy can be exploited to identify therapeutic targets for inherited disorders. We explored this possibility in DYT1 dystonia, a neurodevelopmental movement disorder caused by a loss-of-function (LOF) mutation in the TOR1A gene encoding torsinA. Prior work demonstrates that torsinA and its paralog torsinB have conserved functions at the nuclear envelope. This work established that low neuronal levels of torsinB dictate the neuronal selective phenotype of nuclear membrane budding. Here, we examined whether torsinB expression levels impact the onset or severity of abnormal movements or neuropathological features in DYT1 mouse models. We demonstrate that torsinB levels bidirectionally regulate these phenotypes. Reducing torsinB levels causes a dose-dependent worsening whereas torsinB overexpression rescues torsinA LOF-mediated abnormal movements and neurodegeneration. These findings identify torsinB as a potent modifier of torsinA LOF phenotypes and suggest that augmentation of torsinB expression may retard or prevent symptom development in DYT1 dystonia.

Data availability

Our study did not generate sequencing or structural data. All source data files have been provided.

Article and author information

Author details

  1. Jay Li

    Department of Neurology, University of Michigan, Ann Arbor, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-8146-4450
  2. Chun-Chi Liang

    Neurology, University of Michigan, Ann Arbor, United States
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-8345-8564
  3. Samuel S Pappas

    Peter O'Donnell Jr Brain Institute, University of Texas Southwestern, Dallas, United States
    For correspondence
    samuel.pappas@utsouthwestern.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-6980-2058
  4. William T Dauer

    Peter O'Donnell Jr Brain Institute, University of Texas Southwestern, Dallas, United States
    For correspondence
    william.dauer@utsouthwestern.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-1775-7504

Funding

Bachmann-Strauss Dystonia and Parkinson Foundation

  • William T Dauer

National Institutes of Health (R01 NS077730)

  • William T Dauer

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Harry T Orr, University of Minnesota, United States

Ethics

Animal experimentation: All procedures complied with national ethical guidelines regarding the use of rodents in scientific research and were approved by the University of Michigan (Protocol #00006600 and Protocol #00008870) and University of Texas Southwestern (Protocol #102767) Institutional Animal Care and Use Committees. Every effort was made to minimize both number of mice utilized as well as suffering.

Version history

  1. Received: December 9, 2019
  2. Accepted: March 23, 2020
  3. Accepted Manuscript published: March 23, 2020 (version 1)
  4. Version of Record published: April 8, 2020 (version 2)

Copyright

© 2020, Li et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Jay Li
  2. Chun-Chi Liang
  3. Samuel S Pappas
  4. William T Dauer
(2020)
TorsinB overexpression prevents abnormal twisting in DYT1 dystonia mouse models
eLife 9:e54285.
https://doi.org/10.7554/eLife.54285

Share this article

https://doi.org/10.7554/eLife.54285

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