Cell and molecular transitions during efficient dedifferentiation
- University College London, United Kingdom
- Ludwig-Maximilians-University Munich, Germany
- Beatson Institute, United Kingdom
Abstract
Dedifferentiation is a critical response to tissue damage, yet is not well understood, even at a basic phenomenological level. Developing Dictyostelium cells undergo highly efficient dedifferentiation, completed by most cells within 24 hours. We use this rapid response to investigate the control features of dedifferentiation, combining single cell imaging with high temporal resolution transcriptomics. Gene expression during dedifferentiation was predominantly a simple reversal of developmental changes, with expression changes not following this pattern primarily associated with ribosome biogenesis. Mutation of genes induced early in dedifferentiation did not strongly perturb the reversal of development. This apparent robustness may arise from adaptability of cells: the relative temporal ordering of cell and molecular events was not absolute, suggesting cell programmes reach the same end using different mechanisms. In addition, although cells start from different fates, they rapidly converged on a single expression trajectory. These regulatory features may contribute to dedifferentiation responses during regeneration.
Data availability
Sequencing data have been deposited to GEO under the accession number GSE144892
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Cell and molecular transitions during efficient dedifferentiationNCBI Gene Expression Omnibus, GSE144892.
Article and author information
Author details
Funding
Wellcome (202867/Z/16/Z)
- Jonathan R Chubb
Medical Research Council (MC_U12266B)
- Jonathan R Chubb
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2020, Nichols et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Cell and molecular transitions during efficient dedifferentiation
eLife 9:e55435.
https://doi.org/10.7554/eLife.55435
