Cell and molecular transitions during efficient dedifferentiation

  1. John ME Nichols
  2. Vlatka Antolovic
  3. Jacob D Reich
  4. Sophie Brameyer
  5. Peggy Paschke
  6. Jonathan R Chubb  Is a corresponding author
  1. University College London, United Kingdom
  2. Ludwig-Maximilians-University Munich, Germany
  3. Beatson Institute, United Kingdom

Abstract

Dedifferentiation is a critical response to tissue damage, yet is not well understood, even at a basic phenomenological level. Developing Dictyostelium cells undergo highly efficient dedifferentiation, completed by most cells within 24 hours. We use this rapid response to investigate the control features of dedifferentiation, combining single cell imaging with high temporal resolution transcriptomics. Gene expression during dedifferentiation was predominantly a simple reversal of developmental changes, with expression changes not following this pattern primarily associated with ribosome biogenesis. Mutation of genes induced early in dedifferentiation did not strongly perturb the reversal of development. This apparent robustness may arise from adaptability of cells: the relative temporal ordering of cell and molecular events was not absolute, suggesting cell programmes reach the same end using different mechanisms. In addition, although cells start from different fates, they rapidly converged on a single expression trajectory. These regulatory features may contribute to dedifferentiation responses during regeneration.

Data availability

Sequencing data have been deposited to GEO under the accession number GSE144892

The following data sets were generated

Article and author information

Author details

  1. John ME Nichols

    Laboratory for Molecular Cell Biology, University College London, London, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
  2. Vlatka Antolovic

    Laboratory for Molecular Cell Biology, University College London, London, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
  3. Jacob D Reich

    Laboratory for Molecular Cell Biology, University College London, London, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
  4. Sophie Brameyer

    Microbiology, Ludwig-Maximilians-University Munich, Martinsried, Germany
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-6779-2343
  5. Peggy Paschke

    Beatson Institute, Glasgow, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
  6. Jonathan R Chubb

    Laboratory for Molecular Cell Biology, University College London, London, United Kingdom
    For correspondence
    j.chubb@ucl.ac.uk
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-6898-9765

Funding

Wellcome (202867/Z/16/Z)

  • Jonathan R Chubb

Medical Research Council (MC_U12266B)

  • Jonathan R Chubb

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Richard Gomer

Publication history

  1. Received: January 23, 2020
  2. Accepted: April 6, 2020
  3. Accepted Manuscript published: April 7, 2020 (version 1)
  4. Version of Record published: April 29, 2020 (version 2)

Copyright

© 2020, Nichols et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. John ME Nichols
  2. Vlatka Antolovic
  3. Jacob D Reich
  4. Sophie Brameyer
  5. Peggy Paschke
  6. Jonathan R Chubb
(2020)
Cell and molecular transitions during efficient dedifferentiation
eLife 9:e55435.
https://doi.org/10.7554/eLife.55435

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