Quantitative modeling of the effect of antigen dosage on B-cell affinity distributions in maturating germinal centers
- PSL Research and CNRS, France
- ETH, Switzerland
- ESPCI, PSL Research and CNRS, France
- École Normale Supérieure, France
Abstract
Affinity maturation is a complex dynamical process allowing the immune system to generate antibodies capable of recognizing antigens. We introduce a model for the evolution of the distribution of affinities across the antibody population in germinal centers. The model is amenable to detailed mathematical analysis, and gives insight on the mechanisms through which antigen availability controls the rate of maturation and the expansion of the antibody population. It is also capable, upon maximum-likelihood inference of the parameters, to reproduce accurately the distributions of affinities of IgG-secreting cells we measure in mice immunized against Tetanus Toxoid under largely varying conditions (antigen dosage, delay between injections). Both model and experiments show that the average population affinity depends non-monotonically on the antigen dosage. We show that combining quantitative modelling and statistical inference is a concrete way to investigate biological processes underlying affinity maturation (such as selection permissiveness), hardly accessible through measurements.
Data availability
All the data analysed in this work are reported in the supporting excel file attached to the submission. These data come from (1) new experiments reported in the present work, and (2) previously published experiments, see Eyer et al., 2017 (referenced in manuscript). The code containing the implementation of our stochastic and deterministic model is made publicly available in the following repository: https://github.com/mmolari/affinity_maturation. The repository also includes the experimental dataset, the code to run the inference procedure and the code to reproduce the figures of the main paper (Fig. 2 to 6). Please refer to README.md file for further details.
Article and author information
Author details
Funding
H2020 European Research Council (80336)
- Klaus Eyer
Agence Nationale de la Recherche (CE30-0021-01 RBMPro)
- Rémi Monasson
Agence Nationale de la Recherche (ANR-10-LABX-31)
- Jean Baudry
Agence Nationale de la Recherche (ANR- 10-EQPX-34)
- Jean Baudry
Agence Nationale de la Recherche (ANR-10-IDEX-0001-02 PSL)
- Jean Baudry
- Simona Cocco
- Rémi Monasson
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Animal experimentation: Experiments using mice were validated by the CETEA ethics committee number 89 (Institut Pasteur, Paris, France) under #2013-0103, and by the French Ministry of Research under agreement #00513.02.
Copyright
© 2020, Molari et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 2,087
- views
-
- 348
- downloads
-
- 26
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Citations by DOI
-
- 26
- citations for umbrella DOI https://doi.org/10.7554/eLife.55678
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Quantitative modeling of the effect of antigen dosage on B-cell affinity distributions in maturating germinal centers
eLife 9:e55678.
https://doi.org/10.7554/eLife.55678
