Self-restoration of cardiac excitation rhythm by anti-arrhythmic ion channel gating
- Laboratory of Experimental Cardiology, Department of Cardiology, Leiden University Medical Center, Netherlands
- Department of Physics and Astronomy, Ghent University, Belgium
- Department of Cardiovascular Sciences, KU Leuven, Belgium
- Department of Medical Biology, Amsterdam UMC, Netherlands
- Department of Experimental Cardiology, Amsterdam UMC, Netherlands
- Laboratory of Computational Biology and Medicine, Ural Federal University, Russian Federation
Figures
Figure 1
Anti-arrhythmic action of the BioICD channel (Model I, see below).
(A) Development of a sustained tachyarrhythmia, induced via an extrasystole, in a simulated monolayer of normal human ventricular tissue (TNNP model; ten Tusscher and Panfilov, 2006). (B) …
Figure 2
Schematic diagram and behaviour of BioICD channel Model I.
(A) Markov diagram for BioICD channel Model I. (B) and (C) Traces of voltage () and state variables and , at 1.2 Hz and 4.2 Hz, respectively. : open state of the frequency-sensing channel …
Figure 3
Self-restoration of excitation rhythm in human ventricular monolayers with reentrant tachyarrhythmias (TNNP model [ten Tusscher and Panfilov, 2006]) for four different pathological substrates.
(A) A substrate for VT. (B) A substrate for VF. (C) A substrate with diffuse fibrosis. (D) A substrate with an anatomical scar. In each panel, successive frames (from left to right) show the voltage …
Figure 4
Self-restoration of excitation rhythm in anatomically realistic models of human (A) atria and (B) ventricles with reentrant arrhythmias using BioICD channel Model I (more depolarised regions are more transparent).
The upper panels show representative plots of the control situation (in the absence of a BioICD channel). Induction, during sinus rhythm, of reentrant activity by giving an extra external stimulus …
Figure 5
Self-restoration of excitation rhythm in cultured human atrial myocytes (AM) with the help of dynamic patch-clamping using BioICD channel Model I.
(A) Human atrial myocytes stained for (from left to right) -actinin (ACTN2), enhanced yellow fluorescent protein (eYFP) and DNA. (B) Dynamic patch-clamp set-up creating a feedback system for …
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Figure 5—source data 1
Dynamic clamp reading 1: CSV-file containing the raw data for the C-panel in Figure 5.
There are 22 columns, the first 11 for the voltage measurement, the next 11 for the injected current measurement. The first column indicates the time, the subsequent columns are measurements that follow each other in time.
- https://cdn.elifesciences.org/articles/55921/elife-55921-fig5-data1-v2.csv
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Figure 5—source data 2
Dynamic clamp reading 2: CSV-file containing the raw data for the D-panel in Figure 5.
The data structure is similar to the one from the previous raw data file.
- https://cdn.elifesciences.org/articles/55921/elife-55921-fig5-data2-v2.csv
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Figure 5—source data 3
Dynamic clamp summary table: Table containing the experimental datapoints that were plotted in Figure 5G.
- https://cdn.elifesciences.org/articles/55921/elife-55921-fig5-data3-v2.csv
Appendix 1—figure 1
Schematic diagram and behaviour of BioICD channel Model II.
(A) Markov diagram for BioICD Model II. (B) and (C) Traces of voltage () and state variables at 1.2 Hz and 4.2 Hz, respectively.
Appendix 1—figure 2
Schematic diagram and behaviour of BioICD channel Model III.
(A) Markov diagram for BioICD Model III. (B) and (C) Traces of voltage () and state variables at 1.2 Hz and 4.2 Hz, respectively.
Appendix 1—figure 3
Anti-arrhythmic action of BioICD Model II and III in monolayers of human ventricular tissue (TNNP model).
(A and B) Pseudocolor plots of subsequent time frames of the process of biological auto-detection and termination of reentrant tachyarrhythmias, followed by restoration of sinus rhythm, as …
Author response image 1
1D cable action potential duration (APD) restitution.
(A) Dynamic APD restitution curve for the original parameters of the human ventricular cardiomyocyte model, with and without BioICD channel. (B) Conduction velocity restitution curve for the TNNP …
Videos
Video 1
Self-restoration of cardiac excitation rhythm after VT in monolayers of human ventricular tissue.
The activation of the BioICD current is shown in grey, as an overlay on the voltage map in the BioICD case.
Video 2
Self-restoration of cardiac excitation rhythm after VF in monolayers of human ventricular tissue.
The activation of the BioICD current is shown in grey, as an overlay on the voltage map in the BioICD case.
Video 3
Self-restoration of cardiac excitation rhythm after VT in monolayers of human ventricular tissue, in the presence of 20% fibroblasts.
The activation of the BioICD current is shown in grey, as an overlay on the voltage map in the BioICD case.
Video 4
Self-restoration of cardiac excitation rhythm after VT in monolayers of human ventricular tissue, in the presence of an anatomically realistic scar (3.4 cm long), surrounded by a grey zone, where was reduced by 80%, by 70%, by 69% and by 62%.
The activation of the BioICD current is shown in purple, as an overlay on the voltage map in the BioICD case.
Video 5
Failure of self-restoration of cardiac excitation rhythm after VT in monolayers of human ventricular tissue, in the presence of a large anatomically realistic scar (5.8 cm long), surrounded by a grey zone, where was reduced by 80%, by 70%, by 69% and by 62%.
The activation of the BioICD current is shown in grey, as an overlay on the voltage map in the BioICD case.
Video 6
Self-restoration of cardiac excitation rhythm after VF in anatomically realistic human ventricles.
Video 7
Self-restoration of cardiac excitation rhythm after AF in anatomically realistic human atria.
Tables
Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo-sapiens) | Conditionally immortalised human atrial cell line | doi:10.1093/eurheartj | ||
| Gene (Chlamydomonas reinhardtii) | ChR2(H134R) | Addgene | plasmid #26973 | source of the H134R variant of channelrhodopsin 2 |
| Genetic reagent | LVV-ChR2(H134R) | This paper | Lentiviral vector particles to transduce and express ChR2(H134R) | |
| Software, algorithm | graphics processing unit-usable code | doi:10.1152/ajp-heart.00109.2006, doi:10.1038/srep20835 | ||
| Software, algorithm | RTXI dynamic clamp software | doi:10.1371/journal. pcbi.1005430 |
Additional files
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Source code 1
Key component of the graphics processing unit-usable code.
- https://cdn.elifesciences.org/articles/55921/elife-55921-code1-v2.cu
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Transparent reporting form
- https://cdn.elifesciences.org/articles/55921/elife-55921-transrepform-v2.docx
