Dynamic control of adipose tissue development and adult tissue homeostasis by platelet-derived growth factor receptor alpha
- Department of Physiology and Biophysics, College of Medicine, The University of Illinois, United States
- Department of Pharmacology, College of Medicine, The University of Illinois, United States
- Division of Endocrinology, Department of Medicine, The University of Illinois at Chicago, United States
- Division of Nutritional Sciences, Cornell University, United States
Figures
Figure 1 with 1 supplement
Developmental, but not adult, adipocytes derive from a PDGFRα+ cell source.
(A–B) PdgfraCre-ERT2; Rosa26RRFP (PDGFRα-RFP) mice were administered tamoxifen (TM) (A) at postnatal day 10 (P10) and fed chow or HFD until P60 or (B) at P60 and fed chow or HFD until P120. IGW, …
Figure 1—figure supplement 1
Pdgfra-dependent RFP expression at pulse, P60-P180, and P60-P120 TZD pulse-chase.
(A–B) PdgfraCre-ERT2; Rosa26RRFP (PDGFRα-RFP) mice were administered TM (A) at postnatal day (P) 10 or (B) at P60. IGW, PGW, and BATs were examined for direct RFP fluorescence and RFP IHC staining …
Figure 2 with 1 supplement
Developmental, but not adult, PDGFRα+ cells are adipogenic.
(A–B) PdgfraCre-ERT2; Rosa26RRFP (PDGFRα-RFP) male mice were administered TM at P10 (A) or P60 (B). Stromal vascular fraction of cells (SVF) were isolated from IGW of the mice after 3 days and …
Figure 2—figure supplement 1
Developmental, but not adult, PDGFRα+ cells overlap with PPARγ+ cells.
(A) Schematic of PpargtTA-H2BGFP; PDGFRα-RFP male mice breeding. Mice were administered TM at P10 or P60. SVF were isolated after 3 days. GFP+RFP+ cells were quantified in IGW, PGW, and BAT. (B) The …
Figure 3 with 1 supplement
Developmental PDGFRα+ cells contribute to postnatal but not adult WAT development.
(A) PdgfraCre-ERT2; Rosa26RRFP (PDGFRα-RFP) mice were administered TM (A) at postnatal day (P) 10 and fed chow or HFD until P120. IGW, PGW, and BATs were examined for direct RFP fluorescence and RFP …
Figure 3—figure supplement 1
P10 PDGFRα+ cells contribute to postnatal but not adult WAT development.
(A) PdgfraCre-ERT2; Rosa26RRFP (PDGFRα-RFP) mice were administered TM (A) at postnatal day (P) 10 and fed chow or HFD until P120. IGW, PGW, and BATs were examined for direct RFP fluorescence. Scale =…
Figure 4 with 1 supplement
Deleting Pdgfra in adult SMA+ APCs is dispensable for adult white adipogenesis.
(A–B) Acta2Cre-ERT2; Pdgfrafl/fl male control and mutant (SMA-PDGFRα-KO) mice were administered TM at P60 and analyzed at P90. (C) Body weight of control and SMA-PDGFRα-KO mice at P90. Data are …
Figure 4—figure supplement 1
SMA-PDGFRα-KO mice do not display abnormal energy expenditure.
(A) Body fat mass of Acta2Cre-ERT2; Pdgfrafl/fl male control and SMA-PDGFRα-KO mice at P90. Data are expressed as mean ± SEM. (B) Food intake of control and SMA-PDGFRα-KO mice. Data are expressed as …
Figure 5 with 1 supplement
Deleting Pdgfra in adult SMA+ APCs is dispensable for cold-induced beige adipogenesis.
(A) A 3-month-old Acta2Cre-ERT2; Pdgfrafl/fl male control and SMA-PDGFRα-KO mice were administered TM. After 14 days of TM washout, the mice were cold-exposed for 7 days. (B) Rectal temperature …
Figure 5—figure supplement 1
Deleting Pdgfra in adult SMA+ APCs is dispensable for cold-induced beige adipogenesis.
(A) A 3-month-old Acta2Cre-ERT2; Pdgfrafl/fl male control and SMA-PDGFRα-KO mice were administered TM. After 14 days of TM washout, the mice were cold-exposed for 7 days. H&E staining of BAT. Scale =…
Figure 6 with 1 supplement
PDGFRα in developmental APCs is essential for adipose tissue development.
(A) A 2-month-old PpargtTA; TRE-Cre; TRE-H2B-GFP; Pdgfrafl/fl male control and AT-PDGFRα-KO mice were analyzed. (B) Body weight. Data are expressed as mean ± SEM. (C) IGW and PGW tissue. (D) Adipose …
Figure 6—figure supplement 1
PDGFRα in developmental APCs is essential for adipose tissue development.
(A) IGW tissues of 2-month-old PpargtTA; TRE-Cre; TRE-H2B-GFP; Pdgfrafl/fl male control and AT-PDGFRα-KO mice. (B) Hematoxylin and eosin (H&E) staining of ISCW and liver of control and AT-PDGFRα-KO …
Figure 7 with 1 supplement
PDGFRα regulates adipose tissue development through lineage control.
(A) A 2-month-old PpargtTA; TRE-Cre; TRE-H2B-GFP; Pdgfrafl/fl male control and AT-PDGFRα-KO mice were analyzed. Trichrome staining of IGW. Scale = 200 μm. (B) Direct GFP fluorescence of IGW. Scale = …
Figure 7—figure supplement 1
Pdgfra deletion in developmental APCs.
IGW tissues of P60 PpargtTA; TRE-Cre; TRE-H2B-GFP; Pdgfrafl/fl male control and AT-PDGFRα-KO mice. White arrowheads indicate the GFP+ progenitors align on the vasculature.
Additional files
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Supplementary file 1
Primer sequences used.
- https://cdn.elifesciences.org/articles/56189/elife-56189-supp1-v2.docx
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Transparent reporting form
- https://cdn.elifesciences.org/articles/56189/elife-56189-transrepform-v2.docx
