(a) Two-dimensional sketch of a parasite with a directional vector from the parasite’s back at to its apex at . (b) Three-dimensional triangulated surfaces of a RBC (red) and a parasite …
(a) A time instance of parasite motion at RBC membrane from an experimental video (Weiss et al., 2015) (top) and simulation (bottom), see also Video 1. To obtain the distribution of merozoite …
Source data for graphs shown in Figure 2(b,c).
(a) Sketch of apex distance and alignment angle . The apex distance is defined as a distance (magenta line) between the parasite’s apex and the closest vertex of RBC membrane. The alignment …
Source data for graphs shown in Figure 3b,c and Figure 3—figure supplement 1.
Probability distributions of (a) the apex distance , and (b) the alignment angle . The dashed line in the apex distance distribution indicates the cutoff of repulsive LJ interactions.
(a) Two-dimensional probability map as a function of and . Each bin represents a single alignment state and the color corresponds to probability of that state. The dark green area ( and , …
Source data for graphs shown in Figure 4a–c.
Temporal changes in the number of bonds are shown for both long and short bond types. The dashed lines in the bottom plot correspond to the alignment criteria in Equation 4. For all quantities, the …
Source data for graphs shown in Figure 5 and Figure 5—figure supplements 1 and 2.
(a) Average total number of bonds between the merozoite and RBC as a function of the distance between their centers of mass. (b) Illustration of parasite adhesion at the RBC rim (marked by I) and …
Since the off-rate controls the lifetime of bonds, a smaller off-rate results in a stronger adhesion, a lower parasite displacement, and a faster alignment time.
Source data for graphs shown in Figure 6a–c and Figure 6—figure supplement 1.
(a) RBC deformation energy and (b) the number of short and long bonds as a function of . corresponds to the reference case with parameters given in Table 2. Note that both and are changed …
Source data for graphs shown in Figure 7a,b and Figure 7—figure supplement 1.
(a) Number of short and long bonds and (b) parasite alignment times as a function of . Note that remains constant in all simulations. Here, the bond kinetic rates are , , and . In case of …
Source data for graphs shown in Figure 8a,b and Figure 8—figure supplements 1 and 2.
(a) Deformation energy, (b) the number of bonds, (c) apex distance, (d) alignment angle, and (e) fixed-time displacement of the merozoite for the three cases: (1) and , (2) and (the …
Note that for a rigid RBC with , parasite alignment time could not be computed through the MC sampling, as the alignment criteria have never been met in direct simulations.
Source data for graphs shown in Figure 9a,b.
. See Figure 2a.
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The effective RBC diameter sets a basic length, the thermal energy defines an energy scale, and RBC relaxation time sets a time scale in the simulated system, where is the RBC surface …
Parameter | Simulation value | Physical value |
---|---|---|
133.5 | ||
0.01 | ||
0.92 s | ||
1.85 | ||
µ | ||
1230 | ||
3000 | ||
The parameter values in simulations are given in terms of the length scale , energy scale , and timescale . The densities of long and short ligands are given in terms of parasite vertex …
Parameter | Simulation value | Physical value |
---|---|---|
0.4 | ||
0.6 | ||