1. Computational and Systems Biology

Breakage of the Oligomeric CaMKII Hub by the Regulatory Segment of the Kinase

  1. Deepti Karandur
  2. Moitrayee Bhattacharyya
  3. Zijie Xia
  4. Young Kwang Lee
  5. Serena Muratcioglu
  6. Darren McAffee
  7. Ethan McSpadden
  8. Baiyu Qiu
  9. Jay T Groves
  10. Evan R Williams  Is a corresponding author
  11. John Kuriyan  Is a corresponding author
  1. Howard Hughes Medical Institute, University of California, Berkeley, United States
  2. University of California, Berkeley, United States
  3. San Diego State University, United States
https://doi.org/10.7554/eLife.57784
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  1. Deepti Karandur
  2. Moitrayee Bhattacharyya
  3. Zijie Xia
  4. Young Kwang Lee
  5. Serena Muratcioglu
  6. Darren McAffee
  7. Ethan McSpadden
  8. Baiyu Qiu
  9. Jay T Groves
  10. Evan R Williams
  11. John Kuriyan
(2020)
Breakage of the Oligomeric CaMKII Hub by the Regulatory Segment of the Kinase
eLife 9:e57784.
https://doi.org/10.7554/eLife.57784
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Abstract

Ca2+/calmodulin dependent protein kinase II (CaMKII) is an oligomeric enzyme with crucial roles in neuronal signaling and cardiac function. Previously, we showed that activation of CaMKII triggers the exchange of subunits between holoenzymes, potentially increasing the spread of the active state (Stratton et al. 2014; Bhattacharyya et al. 2016). Using mass spectrometry, we show now that unphosphorylated and phosphorylated peptides derived from the CaMKII-α regulatory segment bind to the CaMKII-α hub and break it into smaller oligomers. Molecular dynamics simulations show that the regulatory segments dock spontaneously at the interface between hub subunits, trapping large fluctuations in hub structure. Single-molecule fluorescence intensity analysis of CaMKII-α expressed in mammalian cells shows that activation of CaMKII-α results in the destabilization of the holoenzyme. Our results suggest that release of the regulatory segment by activation and phosphorylation allows it to destabilize the hub, producing smaller assemblies that might reassemble to form new holoenzymes.

Data availability

Molecular dynamics simulation trajectories will be made available by Pittsburg Supercomputing Center's data storage facility and will be accessible at the following link: https://psc.edu/anton-project-summaries?id=3071&pid=35

Article and author information

Author details

  1. Deepti Karandur

    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United States
    Competing interests
    No competing interests declared.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-6949-6337

  2. Moitrayee Bhattacharyya

    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States
    Competing interests
    No competing interests declared.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-2168-1541

  3. Zijie Xia

    Department of Chemistry, University of California, Berkeley, Berkeley, United States
    Competing interests
    No competing interests declared.

  4. Young Kwang Lee

    Department of Molecular and Cell Biology, San Diego State University, San Diego, United States
    Competing interests
    No competing interests declared.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-0056-6357

  5. Serena Muratcioglu

    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States
    Competing interests
    No competing interests declared.

  6. Darren McAffee

    Department of Molecular and Cell Biology, Department of Chemistry, University of California, Berkeley, Berkeley, United States
    Competing interests
    No competing interests declared.

  7. Ethan McSpadden

    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States
    Competing interests
    No competing interests declared.

  8. Baiyu Qiu

    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States
    Competing interests
    No competing interests declared.

  9. Jay T Groves

    QB3, University of California, Berkeley, Berkeley, United States
    Competing interests
    No competing interests declared.

  10. Evan R Williams

    Department of Chemistry, University of California, Berkeley, Berkeley, United States
    For correspondence
    erw@berkeley.edu
    Competing interests
    No competing interests declared.

  11. John Kuriyan

    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States
    For correspondence
    jkuriyan@mac.com
    Competing interests
    John Kuriyan, Senior editor, eLife.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-4414-5477

Funding

National Institute of General Medical Sciences (K99 GM 126145)

  • Moitrayee Bhattacharyya

National Science Foundation (CHE-1609866)

  • Zijie Xia

National Science Foundation (CHE-1609866)

  • Evan R Williams

Howard Hughes Medical Institute

  • John Kuriyan

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Copyright

© 2020, Karandur et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Deepti Karandur
  2. Moitrayee Bhattacharyya
  3. Zijie Xia
  4. Young Kwang Lee
  5. Serena Muratcioglu
  6. Darren McAffee
  7. Ethan McSpadden
  8. Baiyu Qiu
  9. Jay T Groves
  10. Evan R Williams
  11. John Kuriyan
(2020)
Breakage of the Oligomeric CaMKII Hub by the Regulatory Segment of the Kinase
eLife 9:e57784.
https://doi.org/10.7554/eLife.57784

Share this article

https://doi.org/10.7554/eLife.57784

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Further reading

    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Molecular mechanism of activation-triggered subunit exchange in Ca2+/calmodulin-dependent protein kinase II

    Moitrayee Bhattacharyya, Margaret M Stratton ... John Kuriyan
    Research Article Updated

    Activation triggers the exchange of subunits in Ca2+/calmodulin-dependent protein kinase II (CaMKII), an oligomeric enzyme that is critical for learning, memory, and cardiac function. The mechanism by which subunit exchange occurs remains elusive. We show that the human CaMKII holoenzyme exists in dodecameric and tetradecameric forms, and that the calmodulin (CaM)-binding element of CaMKII can bind to the hub of the holoenzyme and destabilize it to release dimers. The structures of CaMKII from two distantly diverged organisms suggest that the CaM-binding element of activated CaMKII acts as a wedge by docking at intersubunit interfaces in the hub. This converts the hub into a spiral form that can release or gain CaMKII dimers. Our data reveal a three-way competition for the CaM-binding element, whereby phosphorylation biases it towards the hub interface, away from the kinase domain and calmodulin, thus unlocking the ability of activated CaMKII holoenzymes to exchange dimers with unactivated ones.