Conservation of peripheral nervous system formation mechanisms in divergent ascidian embryos
- CNRS, France
- Albert Einstein college of medicine, United States
- Osaka University, Japan
Abstract
Ascidians with very similar embryos but highly divergent genomes are thought to have undergone extensive developmental system drift. We compared, in four species (Ciona and Phallusia for Phlebobranchia, Molgula and Halocynthia for Stolidobranchia), gene expression and gene regulation for a network of six transcription factors regulating peripheral nervous system (PNS) formation in Ciona. All genes, but one in Molgula, were expressed in the PNS with some differences correlating with phylogenetic distance. Cross-species transgenesis indicated strong levels of conservation, except in Molgula, in gene regulation despite lack of sequence conservation of the enhancers. Developmental system drift in ascidians is thus higher for gene regulation than for gene expression; and is impacted not only by phylogenetic distance, but also in a clade-specific manner and unevenly within a network. Finally, considering that Molgula is divergent in our analyses, this suggests deep conservation of developmental mechanisms in ascidians after 390 My of separate evolution.
Data availability
All data generated or analyzed during this study are included in the manuscript and supporting files.
Article and author information
Author details
Funding
Agence Nationale de la Recherche (ANR-11-JSV2-007)
- Sébastien Darras
Agence Nationale de la Recherche (ANR-17-CE13-0027)
- Sébastien Darras
Fondation des Treilles
- Joshua F Coulcher
Centre National de la Recherche Scientifique (DBM2020)
- Sébastien Darras
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Shigehiro Kuraku, RIKEN Center for Biosystems Dynamics Research, Japan
Version history
- Received: May 21, 2020
- Accepted: November 13, 2020
- Accepted Manuscript published: November 16, 2020 (version 1)
- Version of Record published: December 2, 2020 (version 2)
Copyright
© 2020, Coulcher et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Conservation of peripheral nervous system formation mechanisms in divergent ascidian embryos
eLife 9:e59157.
https://doi.org/10.7554/eLife.59157
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