Orderly assembly underpinning built-in asymmetry in the yeast centrosome duplication cycle requires cyclin-dependent kinase
- University of Cambridge, United Kingdom
- Stowers Institute for Medical Research, United States
Abstract
Asymmetric astral microtubule organization drives the polarized orientation of the S. cerevisiae mitotic spindle and primes the invariant inheritance of the old spindle pole body (SPB, the yeast centrosome) by the bud. This model has anticipated analogous centrosome asymmetries featured in self-renewing stem cell divisions. We previously implicated Spc72, the cytoplasmic receptor for the gamma-tubulin nucleation complex, as the most upstream determinant linking SPB age, functional asymmetry and fate. Here we used structured illumination microscopy and biochemical analysis to explore the asymmetric landscape of nucleation sites inherently built into the spindle pathway and under the control of cyclin-dependent kinase (CDK). We show that CDK enforces Spc72 asymmetric docking by phosphorylating Nud1/centriolin. Furthermore, CDK-imposed order in the construction of the new SPB promotes the correct balance of nucleation sites between the nuclear and cytoplasmic faces of the SPB. Together these contributions by CDK inherently link correct SPB morphogenesis, age and fate.
Data availability
Original data has been made available via the Stowers institutional server at ftp://odr.stowers.org/LIBPB-1526For phosphoproteomic datasets cited in this manuscript, complete details are included in the citation and reference list. The datasets are accessible as supplemental material at the journal sites.
Article and author information
Author details
Funding
NIH -NIGMS (RO1GM121443)
- Sue L Jaspersen
CSC Cambridge International Scholarship
- Qiuran Peng
- Marisa Segal
CSC Cambridge International Scholarship
- Zhiang Guo
- Marisa Segal
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2020, Geymonat et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Orderly assembly underpinning built-in asymmetry in the yeast centrosome duplication cycle requires cyclin-dependent kinase
eLife 9:e59222.
https://doi.org/10.7554/eLife.59222
