Live imaging of hair bundle polarity acquisition demonstrates a critical timeline for transcription factor Emx2

  1. Yosuke Tona
  2. Doris K Wu  Is a corresponding author
  1. NIDCD, NIH, United States

Abstract

The asymmetric hair bundle on top of hair cells (HCs), comprises a kinocilium and stereocilia staircase, dictates HC's directional sensitivity. The mother centriole (MC) forms the base of the kinocilium, where stereocilia are subsequently built next to it. Previously we showed that transcription factor Emx2 reverses hair bundle orientation and its expression in the mouse vestibular utricle is restricted, resulting in two regions of opposite bundle orientation (Jiang et al, 2017). Here, we investigated establishment of opposite bundle orientation in embryonic utricles by live-imaging GFP-labeled centrioles in HCs. The daughter centriole invariably migrated ahead of the MC from the center to their respective peripheral locations in HCs. Comparing HCs between utricular regions, centriole trajectories were similar but they migrated towards opposite directions, suggesting that Emx2 pre-patterned HCs prior to centriole migration. Ectopic Emx2, however, reversed centriole trajectory within hours during a critical time-window when centriole trajectory was responsive to Emx2.

Data availability

The following figures contain the source data files. Figure 2 (source data 1), Figure 2 supplement 2 (source data 1-3), Figure 2 supplement 3 (source data 1-4), Figure 2 supplement 4 (source data 1-2), Figure 3 (source data 1-2), figure 3 supplement 2 (source data 1), Figure 4 (source data 1-2), Figure 5 (source data 1), Figure 6 (source data 1-2), Figure 7 (source data 1), Figure 7 supplement 1 (source data 1), Figure 8 (source data 1-2), Figure 9 (source data 1).

Article and author information

Author details

  1. Yosuke Tona

    Section on Sensory Cell Regeneration and Development, Laboratory of Molecular Biology, NIDCD, NIH, Bethesda, United States
    Competing interests
    No competing interests declared.
  2. Doris K Wu

    Section on Sensory Cell Regeneration and Development, Laboratory of Molecular Biology, NIDCD, NIH, Bethesda, United States
    For correspondence
    wud@nidcd.nih.gov
    Competing interests
    Doris K Wu, Reviewing editor, eLife.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-1400-3558

Funding

National Institutes of Health (1ZIADC000021)

  • Yosuke Tona

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Tanya T Whitfield, University of Sheffield, United Kingdom

Ethics

Animal experimentation: All animal experiments were conducted according to NIH guidelines and under the approved Animal Care Protocol of NIDCD/NIH (#1212-17).

Version history

  1. Received: May 28, 2020
  2. Accepted: September 17, 2020
  3. Accepted Manuscript published: September 23, 2020 (version 1)
  4. Version of Record published: October 5, 2020 (version 2)

Copyright

This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

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  1. Yosuke Tona
  2. Doris K Wu
(2020)
Live imaging of hair bundle polarity acquisition demonstrates a critical timeline for transcription factor Emx2
eLife 9:e59282.
https://doi.org/10.7554/eLife.59282

Share this article

https://doi.org/10.7554/eLife.59282

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