A physical mechanism of TANGO1-mediated bulky cargo export
- The Barcelona Institute of Science and Technology, Spain
- Universitat Politècnica de Catalunya-BarcelonaTech, Spain
Abstract
The endoplasmic reticulum (ER)-resident protein TANGO1 assembles into a ring around ER exit sites (ERES), and links procollagens in the ER lumen to COPII machinery, tethers, and ER-Golgi intermediate compartment (ERGIC) in the cytoplasm (Raote et al., 2018). Here we present a theoretical approach to investigate the physical mechanisms of TANGO1 ring assembly and how COPII polymerization, membrane tension, and force facilitate the formation of a transport intermediate for procollagen export. Our results indicate that a TANGO1 ring, by acting as a linactant, stabilizes the open neck of a nascent COPII bud. Elongation of such a bud into a transport intermediate commensurate with bulky procollagens is then facilitated by two complementary mechanisms: (i) by relieving membrane tension, possibly by TANGO1-mediated fusion of retrograde ERGIC membranes; and (ii) by force application. Altogether, our theoretical approach identifies key biophysical events in TANGO1-driven procollagen export.
Data availability
All data generated or analysed during this study are included in the manuscript and supporting files.
Article and author information
Author details
Funding
Government of Spain (Severo Ochoa" Programme (CEX2019-000910-S)")
- Morgan Chabanon
- Maria F Garcia-Parajo
- Felix Campelo
Spanish Government (BFU2013-44188-P)
- Ishier Raote
- Vivek Malhotra
Spanish Government (Consolider CSD2009-00016)
- Ishier Raote
- Vivek Malhotra
Spanish Government (Severo Ochoa Program SEV-2012-0208)
- Ishier Raote
- Vivek Malhotra
Spanish Government (Maria de Maeztu MDM-2015-0502)
- Vivek Malhotra
BIST (Ignite grant eTANGO)
- Ishier Raote
- Maria F Garcia-Parajo
- Vivek Malhotra
- Felix Campelo
Spanish Ministry of Science and Innovation (IJCI-2017-34751)
- Ishier Raote
Spanish Ministry of Science and Innovation (RYC-2017-22227)
- Felix Campelo
Europen Comission (CoG-681434)
- Nikhil Walani
- Marino Arroyo
Generalitat de Catalunya (2017-SGR-1278)
- Marino Arroyo
ICREA (ICREA academia)
- Marino Arroyo
Government of Spain (BFU2015-73288-JIN)
- Maria F Garcia-Parajo
- Felix Campelo
Spanish Government (Severo Ochoa Program,CEX2018-000797-S)
- Marino Arroyo
State Research Agency (PID2019-106232RB-I00/ 10.13039/501100011033)
- Morgan Chabanon
- Felix Campelo
Government of Spain (FIS2015-63550-R)
- Maria F Garcia-Parajo
- Felix Campelo
Government of Spain (FIS2017-89560-R)
- Morgan Chabanon
- Maria F Garcia-Parajo
- Felix Campelo
Fundacio Privada Cellex
- Morgan Chabanon
- Maria F Garcia-Parajo
- Felix Campelo
Fundacio Privada Mir-Puig
- Morgan Chabanon
- Maria F Garcia-Parajo
- Felix Campelo
Generalitat de Catalunya (CERCA program)
- Ishier Raote
- Morgan Chabanon
- Maria F Garcia-Parajo
- Vivek Malhotra
- Felix Campelo
European Comission (ERC Advanced Grant (GA 788546))
- Morgan Chabanon
- Maria F Garcia-Parajo
- Felix Campelo
LaserLab 4 Europe (GA 654148)
- Morgan Chabanon
- Maria F Garcia-Parajo
- Felix Campelo
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2020, Raote et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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A physical mechanism of TANGO1-mediated bulky cargo export
eLife 9:e59426.
https://doi.org/10.7554/eLife.59426
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