Systematic functional analysis of rab GTPases reveals limits of neuronal robustness to environmental challenges in flies

(A) Expression notes on optic lobe expression at 40% pupal development. (B) Expression notes on adult brains. The expression patterns are shown in Figure 1—figure supplements 2–3.

For the human protein atlas (www.proteinatlas.org based on Fagerberg et al., 2014) 27 tissues were analyzed. The data was summarized in the following way: “ubiquitous” (detected in all tissue/region/cell types), “widespread” (detected in at least a third but not all tissue/region/cell types), “restricted” (detected in more than one but less than one third of tissue/region/cell types). The classifications “tissue specific”, “tissue enriched”, “group enriched” and “uncertain” were used as described in the human protein atlas. Regarding the data of the mouse embryo (E 14.5) transcriptome atlas (www.eurexpress.org based on Diez-Roux et al., 2011) the original classifications were adopted: “regional signal” (signal detected in a limited number of discrete locations), “no regional signal” (in all tissues or not detectable) or “not detected”. Out of the analyzed tissues “brain, spinal cord, CNS nerves, peripheral nervous system, ganglia” were grouped as nervous system and “gut, stomach, liver, pancreas” as intestines. For the flyatlas2 (www.flyatlas.gla.ac.uk, see also based on Leader et al., 2018) only data of female adults were considered. “Head, brain and thoracicoabdominal ganglion” were grouped as “nervous system high”. The following abbreviations were used: human (H), rodent (R), Drosophila melanogaster (Dm), embryo (E), larva (L), adult (A), Mus musculus (Mm), Rattus norvegicus (Rn), Oryctolagus cuniculus (Oc), cell culture (CC). Asterisks indicate if the Rab is specific to Hominidae (*), specific to primates (**) or specific to primates and dolphins (***).

Mouse knockout models were listed for the mammalian rab GTPase mutants. Among primary publications, the International Mouse Phenotype Consortium (https://www.mousephenotype.org/) was used for information on the viability of mouse knockout models Information on Drosophila mutant viability is based on this study, if not stated otherwise in the table. Only viability / lethality for homozygous mutants was listed. The following abbreviations were used: Drosophila melanogaster (Dm), endoplasmic reticulum (ER), glucose transporter type 4 (GLUT4), insulin-producing cells (IPCs), Jun-N-terminal kinase (JNK), knockout (KO), mammals (M), matrix metalloproteinases (MMP), multivesicular bodies(MVBs), neuromuscular junction (NMJ), planar cell polarity (PCP), plasma membrane (PM), Saccharomyces cerevisiae(Sc), trans-Golgi network (TGN), 37tyrosinase-related protein-1 (Tyrp-1), ventral nerve cord (VNC). Asterisks indicate if the Rab isspecific to Hominidae (*), specific to primates (**) or specific to primates and dolphins (***).

(A) Summary of developmental time for wild type and all fertile, homozygous viable rab mutants at 18°C, 25°C, and 29°C. Listed are number of days (after 24 hr of egg collection) until first 1st instar larvae, pupae, or adults appear, as well as total number of adults hatched and number of adults per vial. Days are given in mean ± SEM. (B) Summary of developmental time for wild type and tested backcrossed rab mutants at 18°C, 25°C, and 29°C. Listed are number of days (after 24 hr of egg collection) until first 1st instar larvae, pupae, or adults appear, as well as total number of adults hatched and number of adults per vial. Days are given in mean ± SEM. (C) Summary of developmental time for wild type and tested rab mutants over deficiencies at 18°C, 25°C and 29°C. Listed are number of days (after 24 hr of egg collection) until first 1st instar larvae, pupae, or adults appear, as well as total number of adults hatched and number of adults per vial. Days are given in mean ± SEM.