1. Cell Biology
  2. Developmental Biology
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EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice

  1. Yuki Osawa
  2. Kazuya Murata
  3. Miho Usui
  4. Yumeno Kuba
  5. Hoai Thu Le
  6. Natsuki Mikami
  7. Toshinori Nakagawa
  8. Yoko Daitoku
  9. Kanako Kato
  10. Hossam Hassan Shawki
  11. Yoshihisa Ikeda
  12. Akihiro Kuno
  13. Kento Morimoto
  14. Yoko Tanimoto
  15. Tra Thi Huong Dinh
  16. Ken-ichi Yagami
  17. Masatsugu Ema
  18. Shosei Yoshida
  19. Satoru Takahashi
  20. Seiya Mizuno  Is a corresponding author
  21. Fumihiro Sugiyama
  1. Master’s Program in Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Japan
  2. Laboratory Animal Resource Center, Trans-border Medical Research Center, University of Tsukuba, Japan
  3. School of Medical Sciences, University of Tsukuba, Japan
  4. Ph.D Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, Japan
  5. Division of Germ Cell Biology, National Institute for Basic Biology, National Institutes of Natural Sciences, Japan
  6. Department of Basic Biology, School of Life Science, Graduate University for Advanced Studies (Sokendai), Japan
  7. Department of Comparative and Experimental Medicine, Nagoya City University Graduate School of Medical Sciences, Japan
  8. Doctoral program in Biomedical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Japan
  9. Doctoral program in Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Japan
  10. Department of Stem Cells and Human Disease Models, Research Center for Animal Life Science, Shiga University of Medical Science, Japan
Research Article
Cite this article as: eLife 2021;10:e59759 doi: 10.7554/eLife.59759
7 figures, 1 table and 2 additional files

Figures

Figure 1 with 3 supplements
Confirmation of EXOC1 expression in testes using the PA-Tag knock-in mouse.

(A) Generation of PA-Tag knock-in mice. In the Exoc1PA-C allele, the LG3-linker connected PA-tag gene fragment was knocked-in just before the stop codon of Exoc1 using CRISPR-Cas9. In the Exoc1PA-N allele, the LG3-connected PA-tag gene fragment was knocked in just after the start codon of Exoc1 using CRISPR-Cas12a. Bold letters represent the CRISPR-Cas9 and Cas12a target sequence. (B) Western blotting of PA-Tag antibody demonstrated that each C- and N-terminal PA-tagged EXOC1 protein was expressed in the adult testes (n = 2 in each genotype). (C) Immunofluorescence with PA-Tag antibody. EXOC1 is observed in every cell in the adult testes. The arrowheads indicate Sertoli cells in which the nucleus is eurochromatin with a large nucleolus. Scale bars: 50 μm.

Figure 1—figure supplement 1
Expression of mRNA for each Exocyst subunit and EXOC1 protein in adult mouse male germ cells.

(A) The expression data of each Exocyst subunit were extracted from the open data source, GSE112393 (Green et al., 2018). GC1 is spermatogonia, GC2 and GC3 are preleptotene stages, GC4–GC8 are meiotic spermatocytes, GC9–GC11 are post-meiotic haploid round spermatids, and GC12 is elongating spermatids. All exocyst subunits are expressed in all differentiation stages. Cdh1, Sall4, and Neruog3 are known markers of spermatogonia and were used as positive controls. (B) Sanger sequencing of PA-Tag and LG3 Linker in Exoc1PA-N and Exoc1PA-C alleles. Intended Knock-in sequence was found in each allele. (C) Immunofluorescence of Exoc1+/PA-C adult testis using anti-PA-tag antibody. PA-tagged EXOC1 was observed in the adult male germ cells of interest in this study: undifferentiated spermatogonia (GFRa1+, Rarγ+), differentiating spermatogonia (Kit+), and spermatocyte (γH2AX+). Scale bars: 100 μm.

Figure 1—figure supplement 2
Expression of Exocyst subunits in Sertoli cells of adult mice.

The expression data of each Exocyst subunit in Sertoli cells (from 9 weeks mouse) was extracted from the open data source, GSM3069461 (Green et al., 2018). The y-axis is the expression level of all genes, and the x-axis is the number of genes at that expression level. All Exocyst subunits might be expressed slightly higher than average.

Figure 1—figure supplement 3
The production of Exoc1 flox mice.

The Exoc1 flox strain was from the Exoc1tm1a(EUCOMM)Hmgu mouse. The Exoc1tm1a(EUCOMM)Hmgu allele was changed to the Exoc1tm1c(EUCOMM)Hmgu (is equal to Exoc1flox) allele by mating with the Flpe expression mouse (B6;SJL-Tg(ACTFLPe)9205Dym/J) (Muranishi et al., 2011). Exon 4 of Exoc1 was floxed in this allele. Arrows indicate primers for detecting the flox allele.

Figure 2 with 2 supplements
Impaired spermatogenesis in Exoc1 cKO mice.

(A) PNA-lectin staining of the Exoc1 adult cKO testis. Signals of PNA-lectin, an acrosomal marker was not observed in the Exoc1 cKO testis. Scale bars: 50 μm. (B) The macroscopic images for H and E staining. Normal spermatogenesis was not observed in almost all of the seminiferous tubules in the Exoc1 cKO adult testis. Scale bars: 300 μm. (C) The mesoscopic images for H and E staining of the Exoc1 cKO testis. Large and circular cells, appearing to be aggregates of syncytia (AGS), containing multiple nuclei were observed in the lumen of seminiferous tubules (arrowheads). Scale bars: 100 μm. Control: Exoc1flox/wt:: Nanos3+/Cre adult mice. (D) SEM observation of the Exoc1 cKO adult testis. Intercellular bridges (ICBs) (arrows) were found in the syncytia in the control (Exoc1flox/wt:: Nanos3+/Cre) testis. There were no ICB observed in the AGS of Exoc1 cKO. Scale bars: 5 μm. (E) The serial section overlay image of Exoc1 cKO adult testis. There were γ-H2AX (marker of spermatocyte) signals in the AGS nucleus. Scale bars: 50 μm. (F) A representative immunofluorescence image of an Exoc1 cKO seminiferous tubule. Kit+ syncytia, which are observed in differentiating spermatogonia, have ICB. Scale bars: 50 μm. (G) H and E staining and immunofluorescence with CLDN11. CLDN11-positive Sertoli cell tight junction (SCTJ) divides the space between the basal and the luminal compartment, and AGS are present within the luminal compartment (arrowheads). Scale bars: 50 μm.

Figure 2—figure supplement 1
The occurrence frequency of aggregates of syncytia (AGS) in the Exoc1 cKO.

(A) Classification reference image of cross-sections of seminiferous tubules to evaluate the incidence of AGS. Cross-sections with AGS indicated by arrowheads were classified as ‘Section containing AGS’. Cross-sections with nuclear condensed sperm (including spermatid and spermatozoa) indicated by arrow were classified as ‘Section containing No AGS But Sperm’. Cross-sections with no AGS or Sperm and empty space in the lumen were classified as ‘Section containing neither AGS nor Sperm’. (B) Cross-sections of the seminiferous tubules of Exoc1 cKO and control (Exoc1flox/wt:: Nanos3+/Cre) adult mice (n = 3 in each genotype) were classified into three categories based on the criteria in (A). (C) Graphical representation of the results in (B). (D) Genotypic analysis of blastocysts obtained by in vitro fertilization of sperm from Exoc1 cKO mice with wild-type oocytes revealed no cKO alleles that underwent Cre-LoxP recombination (0/16, n = 2). Control: Exoc1flox/cKO. M5: Marker 5 (Nippon Genetics).

Figure 2—figure supplement 2
Normal meiotic chromosome synapsis in Exoc1 cKO mice.

Meiotic chromosome synapsis was confirmed by immunofluorescence. In pachytene stage, both the homologous pairing (marked by Sycp3) and synaptonemal complex (marked by Sycp1) were normal in Exoc1 cKO and control (wild-type) autosomal chromosomes (n = 2 in each genotype, 8–17 cells in each mouse).

Figure 3 with 2 supplements
EXOC1 regulates ICB formation in cooperation with STX2 and SNAP23.

(A) Co-immunoprecipitation of EXOC1-STX2-SNAP23 complex in vitro. FLAG-tagged mouse EXOC1, HA-tagged mouse STX2, and Myc-tagged mouse SNAP23 were co-overexpressed in HEK293T cells. The binding of the three factors was confirmed in all combinations of Co-IP experiments. FLAG-EGFP, V5-mCherry-HA, and E2 crimson-MYC-His were used as negative controls. (B) Interaction of EXOC1 with SNAP23 in vivo. PA-tagged EXOC1 was co-immunoprecipitated with endogenous SNAP23 in the adult Exoc1PA-N testis. The upper and middle panels show short and long period exposure images, respectively. Arrowhead indicates PA-EXOC1. (C) The macroscopic images for H and E staining. Sperms were found in frequent seminiferous tubules in adult Snap23 cKO mice. Scale bars: 300 μm. Control: Snap23flox/wt:: Nanos3+/Cre mice. (D) The mesoscopic images for H and E staining of Snap23 cKO adult testis. Large and circular cells containing multiple nuclei (arrowheads), appearing to be aggregates of syncytia (AGS) were observed. In contrast with Exoc1 cKO, every seminiferous tubule had sperms with elongated nuclei (arrows). Scale bars: 50 μm. Control: Snap23flox/wt:: Nanos3+/Cre mice. (E) The serial section overlay image of Snap23 cKO testis. Immunofluorescence signals of γ-H2AX were found in AGS. Scale bars: 50 μm. (F) PNA-lectin staining of Snap23 cKO testis. PNA-lectin that was used to detect the acrosome is observed in the lumen of the seminiferous tubule of Snap23 cKO testis. Scale bars: 50 μm.

Figure 3—source data 1

Raw data of the in vitro immunoprecipitation.

https://cdn.elifesciences.org/articles/59759/elife-59759-fig3-data1-v1.pptx
Figure 3—source data 2

Raw data of the in vivo immunoprecipitation.

https://cdn.elifesciences.org/articles/59759/elife-59759-fig3-data2-v1.pptx
Figure 3—source data 3

Occurrence rate of AGS per area in the extracted Section containing AGS.

https://cdn.elifesciences.org/articles/59759/elife-59759-fig3-data3-v1.xlsx
Figure 3—figure supplement 1
The production of Snap23 flox mice.

Snap23 flox mice were from the Snap23tm1a(EUCOMM)Wtsi frozen sperms. Snap23tm1a(EUCOMM)Wtsi frozen sperms were thawed and fertilized to wild-type oocyte in vitro. Flpe mRNA was electroplated into the fertilized embryos. Snap23tm1a(EUCOMM)Wtsi was changed to Snap23tm1c(EUCOMM)Wtsi (equal to Snap23 flox) allele in every newborn. Exon 5 of Snap23 was floxed in this allele. Arrows indicate the primers for the detection of flox allele.

Figure 3—figure supplement 2
The occurrence frequency of aggregates of syncytia (AGS) in the Snap23 cKO.

(A) Results of classifying the cross-section of the seminiferous tubules of adult Snap23 cKO (n = 3) using the same criteria as in Figure 3—figure supplement 1A. (B) Graph is based on Figure 3—figure supplement 1C, with Snap23 cKO data (A) added to it. (C) ‘Section containing AGS’ classified images were extracted and plotted with the area of each cross-section on the X-axis and the number of AGS in each cross-section on the Y-axis.

Figure 4 with 1 supplement
EXOC1 regulates pseudopod elongation via Rac1 inactivation.

(A) A representative image of GFRα1+ undifferentiated spermatogonia in Exoc1 cKO and Stx2 KO. Pseudopod elongation was impaired in Exoc1 cKO, but not in Stx2 KO. Scale bars: 10 μm. (B) Pseudopod length quantification using sections. Average length of GFRα1+ spermatogonia pseudopods in Exoc1 cKO was shorter than that of Stx2 KO and wild type (n = 3 in each genotype, 25–36 cells in each mouse). *p=0.052, **p=1.8 × 10−6, ***p=9.5 × 10−9. one-way ANOVA. (C) Pseudopod length quantification through whole-mount immunofluorescence staining of adult testes. GFRα1+ spermatogonia with elongated pseudopod (white arrows) were frequently observed in control (Exoc1flox/cKO) mice, whereas they were rarely observed in Exoc1 cKO mice. Scale bars: 30 μm. (D) Measurement of the length of pseudopod of Asingle GFRα1+ cells based on whole-mount immunofluorescence images (n = 3 in each genotype, 20 cells in each mouse). *p=7.2 × 10−17, Student’s t-test. Control: Exoc1flox/cKO. (E, F) Measurement of intercellular length in connected Apair (n = 3 in each genotype, 20 intercellular distances in each mouse) or Aaligned (n = 3 in each genotype, 14–19 intercellular distances in each mouse) based on whole-mount immunofluorescence images. *p=3.6 × 10−12, **p=0.00014, Student’s t-test. Control: Exoc1flox/cKO. (G) A representative image of active-Rac1 in GFRα1+ spermatogonia of Exoc1 cKO adult testis. In control mice (Exoc1flox/wt:: Nanos3+/Cre), active-Rac1 signal was lower than the detection limit. Non-polar active-Rac1 signal was detected in Exoc1 cKO adult testis. Scale bars: 5 μm. (H) Quantification of signal intensity of active-Rac1 in GFRα1+ spermatogonia based on immunostaining images. The average intensity in each cell is higher in the Exoc1 cKO group than that in the control group (n = 3 in genotype, 8–10 cells in each mouse). *p=0.000036, Student’s t-test. Control: Exoc1flox/flox.

Figure 4—source data 1

Measurement of the length of the pseudopodia of GFRα1+ cells in section observation.

https://cdn.elifesciences.org/articles/59759/elife-59759-fig4-data1-v1.xlsx
Figure 4—source data 2

Measurement of the length of the pseudopodia of GFRα1+ cells in whole-mount observation.

https://cdn.elifesciences.org/articles/59759/elife-59759-fig4-data2-v1.xlsx
Figure 4—source data 3

Intensity of active Rac1 signal in each cell.

https://cdn.elifesciences.org/articles/59759/elife-59759-fig4-data3-v1.xlsx
Figure 4—figure supplement 1
The production of the Stx2 KO.

(A) Stx2 KO mice generated by the CRISPR-Cas9 genome editing in mouse zygotes. Two CRISPR targets located 482 bp upstream and 148 bp downstream of exon 5 of Stx2. Arrows indicate long and short PCR primers for detecting the deletion of exon 5 of Stx2. (B) Electrophoresis data of PCR genotyping. We used #4, 9, and 15 as the Stx2 KO mice. M5: Marker 5. M6: Marker 6. (C) H and E staining of Stx2 KO testis. Consistent with reported Stx2 null phenotype (Fujiwara et al., 2013), our Stx2 KO also showed aggregates of syncytia (AGS) (arrowheads).

Figure 5 with 1 supplement
The balance of spermatogonial differentiation is perturbed in Exoc1 cKO.

(A) Representative immunofluorescence image of adult Exoc1 cKO seminiferous tubule. GFRα1+ spermatogonia (arrow) density in the cKO was higher than that in control mice. RARγ+ spermatogonia (arrowhead) density decreased in the cKO. Control: Exoc1flox/flox mice. (B) The number of spermatogonia in a cross-section of seminiferous tubule (n = 3 in each genotype, 46–87 sections in each mouse). The number of GFRα1+ cell per section was significantly increased in the cKO. The number of RARγ+ cell in the cKO was significantly smaller than that in control (Exoc1flox/flox) mice. *p=1.9 × 10−7, **p=3.6 × 10−9, ***p=0.035, Student’s t-test.

Figure 5—figure supplement 1
The number of Kit+ differentiating spermatogonia are reduced in Exoc1 cKO.

The number of Kit+ spermatogonia in each one cross-section of seminiferous tubule in adult mice (n = 3 in each genotype, 20 sections in each mouse). *p=0.000012, Student’s t-test. Control: Exoc1flox/flox.

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Tables

Key resources table
Reagent type
(species) or
resource
DesignationSource or
reference
IdentifiersAdditional
information
Strain, strain background (M. musculus)C57BL/6JCharles River Laboratories JapanStock No: 000664
Strain, strain background (M. musculus)Crl:CD1(ICR)Charles River Laboratories Japan
Genetic reagent (M. musculus)Exoc1 floxThis paperThis is from the Exoc1tm1a(EUCOMM)Hmgu. IKMC Project #78575
Genetic reagent (M. musculus)Exoc1PA-CThis paper
Genetic reagent (M. musculus)Exoc1PA-NThis paper
Genetic reagent (M. musculus)Snap23 floxThis paperThis is from the Snap23tm1a(EUCOMM)Wts. Colony Name BLA3054
Genetic reagent (M. musculus)Stx2 KOThis paper
Genetic reagent (M. musculus)Nanos3tm2 (cre)YsaSuzuki et al., 2008Prof. Saga, RIKEN
BRC (RDB13130)
RBRC02568
Genetic reagent (M. musculus)B6;SJL-Tg(ACTFLPe)9205Dym/JThe Jackson LaboratoryStock No: 003800
Cell line
(Human)
HEK293T cellATCCATCC Sales Order: SO0623448FTA Barcode: STRB4056
AntibodyMonoclonal anti-PA-tag, Biotin conjugated (Rat)FUJIFILM Wako ChemicalsCat#017–27731WB (1:500)
AntibodyPolyclonal anti-b-actin (Rabbit)MEDICAL and BIOLOGICAL LABORATORIESCat#PM053WB (1:3000)
AntibodyMonoclonal anti-DYKDDDDK tag (Mouse)FUJIFILM Wako ChemicalsCat# 018–22381WB (1:2000)
AntibodyMonoclonal anti-DYKDDDDK tag (Rat)FUJIFILM Wako ChemicalsCat# 018–23621WB (1:2000)
AntibodyMonoclonal anti-HA-tag (Rabbit)Cell Signaling TechnologyCat#3724WB (1:2000)
AntibodyMonoclonal anti-HA-tag (Mouse)BioLegendCat#901513WB (1:1000)
AntibodyMonoclonal anti-Myc (Mouse)MEDICAL and BIOLOGICAL LABORATORIESCat#M192-3WB (1:1000)
AntibodyMonoclonal anti-SNAP23 (Mouse)Santa Cruz BiotechnologyCat#sc-166244WB (1:1000)
AntibodyAnti-Rat IgG, HRP-linked (Goat)GE HealthcareCat#NA935VWB (1:30000)
AntibodyAnti-Rabbit IgG, HRP-linked (Donkey)GE HealthcareCat#NA934VWB (1:30000)
AntibodyAnti-Mouse IgG, HRP-linked (Sheep)GE HealthcareCat#NA931VWB (1:30000)
AntibodyNormal IgG (Rabbit)FUJIFILM Wako ChemicalsCat#148–09551Co-IP
AntibodyPolyclonal anti-SNAP23 (Rabbit)AbcamCat#ab3340Co-IP
AntibodyMonoclonal anti-PA-tag (Rat)FUJIFILM Wako ChemicalsCat#016–25861IF (1:1000)
AntibodyMonoclonal anti-γH2AX (Mouse)Merck-MilliporeCat#05–636IF (1:100)
AntibodyPolyclonal anti-SYCP1 (Rabbit)Novus BiologicalCat#NB300-299IF (1:50)
AntibodyMonoclonal anti-SYCP3 (Mouse)Santa Cruz BiotechnologyCat#sc-74569IF (1:50)
AntibodyPolyclonal anti-GFRα1 (Goat)R and D systemsCat#AF560IF (1:400 for section)
IF (1:1000 for whole mount)
AntibodyMonoclonal anti-RARγ1 (Rabbit)Cell Signaling TechnologyCat#8965SIF (1:200)
AntibodyMonoclonal anti-active rac1 (Mouse)NewEast BiosciencesCat#26903IF (1:1000)
AntibodyPolyclonal anti-Exoc1 (Rabbit)ProteintechCat#11690–1-APIF (1:50)
AntibodyPolyclonal anti-Exoc1 (Rabbit)Atlas AntibodiesCat#HPA037706IF (1:50)
AntibodyAnti-Goat IgG, Alexa Fluor 488 (Chicken)Thermo Fisher ScientificCat#A21467IF (1:200 for section)
AntibodyAnti-Goat IgG, Alexa Fluor 594 (Chicken)Thermo Fisher ScientificCat#A21468IF (1:400 for whole mount)
AntibodyAnti-Rat IgG, Alexa Fluor 555 (Donkey)AbcamCat#ab150154IF (1:1000)
AntibodyAnti-Mouse IgG, Alexa Fluor 555 (Goat)Thermo Fisher ScientificCat#A28180IF (1:200)
AntibodyAnti-Mouse IgG, Alexa Fluor 555 (Donkey)Thermo Fisher ScientificCat#A31570IF (1:200)
AntibodyAnti-Rabbit IgG, Alexa Fluor 647 (Goat)Thermo Fisher ScientificCat#A27040IF (1:200)
Recombinant DNA reagentpcDNA3.1 (+) Mammalian Expression VectorInvitrogenV79020
Recombinant DNA reagentT7-NLS hCas9-pA plasmidYoshimi et al., 2016RIKEN BRC
(RDB13130)
Recombinant DNA reagentpCAG-FlpeMatsuda and Cepko, 2007Addgene (Plasmid #13787)
Recombinant DNA reagentpT7-Flpe-pAThis paperRIKEN BRC
(RDB16011)
Sequence-based reagentAll primers in Supplementary file 1bThermo Fisher Scientific
Sequence-based reagentAll primers in Supplementary file 1bThermo Fisher Scientific
Sequence-based reagentExoc1 PA-C ssODNIntegrated DNA TechnologiesGAATTCACTATTCAGGACATTCTGGATTATTGCTCCAGCATCGCACAGTCCCACGGCTCAACCAGCGGATCTGGTAAGCCAGGTAGTGGAGAAGGCAGCACCAAGCCTGGCGGCGTCGCCATGCCTGGAGCCGAGGATGATGTCGTGTAAGCCCTAGGAAAGAGGAGAAAGAAGTGAGCATGCATTCTCAGTCCAGCAAA
Sequence-based reagentExoc1 PA-N ssODNIntegrated DNA TechnologiesGGAGGGCAGTGGTTTTGAGAATTATTCTAAATGTTTTTCAGCTGAGAAAAGATGGGCGTCGCCATGCCTGGAGCCGAGGATGATGTCGTGGGCTCAACCAGCGGATCTGGTAAGCCAGGTAGTGGAGAAGGCAGCACCAAGCCTGGCACAGCAATCAAGCATGCGCTGCAGAGAGATATCTTCACACCAAATGATGAACG
Software, algorithmThe R Foundationhttps://www.r-project.org/foundation/
OtherStreptavidin-HRPNichirei BiosciencesCat#426061WB (1:1000 in 2% BSA/TBS-T)
OtherLectin from Arachis hypogaea, FITCSigma-AdrichCat#L7381Lectin staining (1:100)

Additional files

Supplementary file 1

Data of differentiating spermatogonia aggregation and primers for genotyping.

(a) Differentiating spermatogonia are not aggregated. Examination of aggregated Kit+ differentiating spermatogonia in adult Exoc1 cKO mice. Aggregation was determined by observation of immunofluorescence images with anti-Kit antibody. (b) Primers for genotyping The following is a list of primers used for genotyping or vector construction.

https://cdn.elifesciences.org/articles/59759/elife-59759-supp1-v1.xlsx
Transparent reporting form
https://cdn.elifesciences.org/articles/59759/elife-59759-transrepform-v1.docx

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