(a) Experimental timeline and diagram of recording site on fly antenna. (b) Traces of cVa-sensing T1 neurons. The red bar denotes 300 ms cVa exposure. (c) Δ Spikes calculated as cVa-induced activity (1 s during and after cVa) – spontaneous activity (paired two-tailed t-test, p=0.002, n = 7,15,15). (d) Spontaneous activity before, during, and after ethanol exposure. Spontaneous activity calculated as the total number of spikes 10 s prior to cVa delivery/10 s (Pre- vs. Post-5% p=0.002, Pre- vs. Post-30% p<0.0001, During vs. Post-30% p=0.0152, Kruskal-Wallis test with Dunn’s correction). (e), (f), (g) Averaged spikes over time for air, 5% ethanol, and 30% ethanol, respectively. Red bar denotes 300 ms cVa exposure (h) Time constant (τ) of decay of the cVa-induced spikes (Mann-Whitney test, p=0.0043). p>0.05, n.s. (not significant); p<0.05 *; p<0.01 **; p<0.0001 ****. Error bars denote SEM.