Rab5 and Rab11 maintain hematopoietic homeostasis by restricting multiple signaling pathways in Drosophila

  1. Shichao Yu
  2. Fangzhou Luo
  3. Li Hua Jin  Is a corresponding author
  1. Northeast Forestry University, China

Abstract

The hematopoietic system of Drosophila is a powerful genetic model for studying hematopoiesis, and vesicle trafficking is important for signal transduction during various developmental processes; however, its interaction with hematopoiesis is currently largely unknown. In this article, we selected three endosome markers, Rab5, Rab7 and Rab11, that play a key role in membrane trafficking and determined whether they participate in hematopoiesis. Inhibiting Rab5 or Rab11 in hemocytes or the cortical zone (CZ) significantly induced cell overproliferation and lamellocyte formation in circulating hemocytes and lymph glands and disrupted blood cell progenitor maintenance. Lamellocyte formation involves the JNK, Toll, and Ras/EGFR signaling pathways. Notably, lamellocyte formation was also associated with JNK-dependent autophagy. In conclusion, we identified Rab5 and Rab11 as novel regulators of hematopoiesis, and our results advance the understanding of the mechanisms underlying the maintenance of hematopoietic homeostasis as well as the pathology of blood disorders such as leukemia.

Data availability

All data generated or analysed during this study are included in the manuscript and supporting files.

Article and author information

Author details

  1. Shichao Yu

    Department of Genetics, Northeast Forestry University, Harbin, China
    Competing interests
    The authors declare that no competing interests exist.
  2. Fangzhou Luo

    Department of Genetics, Northeast Forestry University, Harbin, China
    Competing interests
    The authors declare that no competing interests exist.
  3. Li Hua Jin

    Department of Genetics, Northeast Forestry University, Harbin, China
    For correspondence
    lhjin2000@hotmail.com
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-5912-9800

Funding

National Natural Science Foundation of China (31772521)

  • Li Hua Jin

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Jiwon Shim, Hanyang University, Republic of Korea

Version history

  1. Received: July 8, 2020
  2. Accepted: February 8, 2021
  3. Accepted Manuscript published: February 9, 2021 (version 1)
  4. Version of Record published: February 18, 2021 (version 2)

Copyright

© 2021, Yu et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Shichao Yu
  2. Fangzhou Luo
  3. Li Hua Jin
(2021)
Rab5 and Rab11 maintain hematopoietic homeostasis by restricting multiple signaling pathways in Drosophila
eLife 10:e60870.
https://doi.org/10.7554/eLife.60870

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https://doi.org/10.7554/eLife.60870

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