1. Cell Biology
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Polo-like kinase acts as a molecular timer that safeguards the asymmetric fate of spindle microtubule-organizing centers

  1. Laura Matellán
  2. Javier Manzano-López
  3. Fernando Monje-Casas  Is a corresponding author
  1. University of Seville, Spain
  2. Spanish National Research Council (CSIC), Spain
Research Article
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Cite this article as: eLife 2020;9:e61488 doi: 10.7554/eLife.61488

Abstract

The microtubules that form the mitotic spindle originate from microtubule organizing centers (MTOCs) located at either pole. After duplication, spindle MTOCs can be differentially inherited during asymmetric cell division in organisms ranging from yeast to humans. Problems with establishing predetermined spindle MTOC inheritance patterns during stem cell division have been associated with accelerated cellular aging and the development of both cancer and neurodegenerative disorders. Here, we expand the repertoire of functions Polo-like kinase family members fulfill in regulating pivotal cell cycle processes. We demonstrate that the Plk1 homolog Cdc5 acts as a molecular timer that facilitates the timely and sequential recruitment of two key determinants of spindle MTOCs distribution, i.e., the γ-tubulin complex receptor Spc72 and the protein Kar9, and establishes the fate of these structures, safeguarding their asymmetric inheritance during Saccharomyces cerevisiae mitosis.

Data availability

All data generated or analyzed during this study are included in the manuscript and supporting files. No new datasets were generated or previously published datasets used in our work. Rich media files such as videos, audio clips or animations were also not created for this article. No further relevant additional data file was provided.

Article and author information

Author details

  1. Laura Matellán

    CABIMER, University of Seville, Seville, Spain
    Competing interests
    The authors declare that no competing interests exist.
  2. Javier Manzano-López

    CABIMER, Spanish National Research Council (CSIC), Seville, Spain
    Competing interests
    The authors declare that no competing interests exist.
  3. Fernando Monje-Casas

    CABIMER, Spanish National Research Council (CSIC), Seville, Spain
    For correspondence
    fernando.monje@cabimer.es
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-3587-2373

Funding

Spanish Ministry of Economy, Industry and Competitiveness and European Union (BFU2013-43718-P)

  • Fernando Monje-Casas

Spanish Ministry of Economy, Industry and Competitiveness and European Union (BFU2016-76642-P)

  • Fernando Monje-Casas

Spanish Ministry of Economy, Industry and Competitiveness and European Union (FPI fellowship)

  • Laura Matellán

Spanish Ministry of Economy, Industry and Competitiveness and European Union (Juan de la Cierva research contract)

  • Javier Manzano-López

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Reviewing Editor

  1. Elçin Ünal, University of California, Berkeley, United States

Publication history

  1. Received: July 28, 2020
  2. Accepted: October 20, 2020
  3. Accepted Manuscript published: November 2, 2020 (version 1)
  4. Version of Record published: November 16, 2020 (version 2)

Copyright

© 2020, Matellán et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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