(A) The conidial suspension of Guy11 (1 × 105 spores/ml) was inoculated in the excised rice sheath of 4-weeks-old rice seedlings LTH and K23. The invasive hyphae growth was observed at 20, 24, 36, …
(A) Phosphorylation of MoOsm1 in Guy11 in infection of LTH. Total proteins were extracted from LTH leaves 0, 8, 20, 24, 48, and 72 hr. Eluted proteins bound to the anti-GFP beads were analyzed by …
(A) Phosphorylation of MoOsm1 in Guy11 under oxidative stress. Immunoblots were performed with total proteins from hyphae of Guy11 treated with H2O2 for 0, 10, 30, and 60 min. MoOsm1 phosphorylation …
(A) Localization of MoOsm1, MoOsm1Y173D, and MoOsm1Y173A in conidia. MoOsm1-GFP, MoOsm1Y173D-GFP, MoOsm1Y173A-GFP, and H1-RFP were observed by confocal fluorescence microscopy. Bars = 5 μm. (B) …
(A) MoOsm1 phosphopeptide (QDPQMTGYVSTR) in the strain, which treated with H2O2 was identified by mass spectrometer analysis and the phosphorylated sites were Thr171 and Tyr-173. (B) Co-IP assay …
(A) Co-IP assay. Western blot analysis of total proteins (T) extracted from MoOsm1-GFP/MoAtf1-FLAG, MoOsm1Y173A-GFP/MoAtf1-FLAG, MoOsm1Y173D-GFP/MoAtf1-FLAG, MoOsm1NES-GFP/MoAtf1-FLAG strains, …
In vitro phosphorylation analysis by the fluorescence detection in the tube (FDIT) method. Purified proteins of MoOsm1-HIS, MoAtf1-GST, and MoAtf1S124A-GST were used for the protein kinase reaction …
ChIP-Seq assays showed that MoAtf1 binds to the promoters of 16 oxidation regulation pathway genes. Output- and input-DNA was visualized in blue and pink. The red arrow indicates gene direction. The …
(A) Y2H analysis of the interaction between MoAtf1 and MoTup1. The pGADT7 and pGBKT7 fused with specific genes were co-introduced into the yeast AH109 strain, and transformants were plated on …
(A) The relative expression levels of putative oxidation regulation pathway genes in ∆Moatf1 mutant were analyzed by qRT-PCR and normalized to that of ACTIN. Error bars represent the standard …
(A) and (B) Excised rice sheaths from 3-week-old rice seedlings were inoculated with conidial suspension (1 × 105 spores/ml). Infectious growth was observed at 24- and 36-hr post-inoculation (hpi). …
Southern blot analysis of the gene knockout mutant with gene-specific probe (probe one for MoPTP1 and probe three for MoPTP2) and hygromycin phosphotransferase (HPH) probe (probe 2). The scheme of Mo…
(A) Comparison of ∆Moptps and wild-type strain in colony morphology. Wild type, the ∆Moptps mutant and complementation strains were cultured at 28°C in darkness for 7 days. (B) Statistical analyses …
(A) Pathogenicity assay. Conidial suspensions were sprayed onto two-week-old rice seedlings (CO-39). Diseased leaves were photographed after 7 days of inoculation. (B) Quantification of lesion type. …
(A) MoOsm1 phosphopeptide (QDPQMTGYVSTR) in the ΔMoptp2/MoOSM1-GFP strain was identified by mass spectrometer analysis and the phosphorylated sites were Tyr-173. (B) The pathogenicity assay on rice …
Appressorium formation rates at different time points were calculated and statistically analyzed. The percentage at a given time was recorded by observing at least 200 conidia for each strain and …
(A) Pathogenicity assay. Conidial suspensions were sprayed onto two-week-old rice seedlings (CO-39). Diseased leaves were photographed after 7 days of inoculation. (B) Dephosphorylation of MoOsm1 …
A model of MoPtp1, MoPtp2, and mutants constructs. Black bars indicate the conserved phosphatase-domain (PTPc) in MoPtp1 and MoPtp2 that were mutanted in ∆Moptp1/MoPTP1∆ptpc and ∆Moptp2/MoPTP2∆ptpc. …
Immunoblots were performed with total proteins from Guy11, ΔMoptp1, and ΔMoptp2 mutant strains without or with 5 mM H2O2 for 10, 30, and 60 min. The MoOsm1 phosphorylation level was detected using …
(A) Expression analysis of MoPTP1 in the ∆Moatf1 mutant and wild-type strains. The expression of MoPTP1 was analyzed by qRT-PCR and normalized to that of actin gene (MGG_03982). MY represents …
(A) The cis-element of MoAtf1 was predicted by MEME. The putative sequence of the cis-element obtained by analyzing the promoters of MoAtf1 binding genes with the MEME program. (B) Schematic …
(A) and (B) MoPTP1 and MoPTP2 expression analysis in Guy11, ∆Moosm1 mutant, and ∆Moosm1/MoAtf1S124D strains treated with H2O2 for 15, 30, and 60 min. Three independent biological experiments were …
Rice generates immunity, including ROS burst during its interaction with M. oryzae. Once host perception, M. oryzae induces MoOsm1 phosphorylation that disintegrates MoOsm1 dimerization leading to …
574 putative binding proteins of MoAtf1 by CHIP assay.
Genomic location and Annotation ID of MoAtf1 binding genes.