Cohesin architecture and clustering in vivo

Abstract
Data availability
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Abstract

Cohesin helps mediate sister chromatid cohesion, chromosome condensation, DNA repair and transcription regulation. We exploited proximity-dependent labeling to define the in vivo interactions of cohesin domains with DNA or with other cohesin domains that lie within the same or in different cohesin complexes. Our results suggest both cohesin's head and hinge domains bind to DNA, and cohesin structure is dynamic with differential folding of its coiled coil regions to generate butterfly confirmations. This method also reveals that cohesins form ordered clusters on and off DNA. The levels of cohesin clusters and their distribution on chromosomes are cell cycle-regulated. Cohesin clustering is likely necessary for cohesion maintenance because clustering and maintenance uniquely require the same subset of cohesin domains and the auxiliary cohesin factor Pds5p. These conclusions provide important new mechanistic and biological insights into the architecture of the cohesin complex, cohesin-cohesin interactions, and cohesin's tethering and loop extruding activities.

Data availability

Sequencing data have been deposited in GEO as GSE157155.

The following data sets were generated

1. Xiang S
2. Koshland D
(2020) Chromosome localization of cohesin oligomers in mid-M arrested yeast cells
NCBI Gene Expression Omnibus, GSE157155.

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE157155

The following previously published data sets were used

(2020) Cohesin residency determines chromatin loop patterns
NCBI Gene Expression Omnibus, GSE151416.

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE151416

Article and author information

Author details

Siheng Xiang

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States

Competing interests
The authors declare that no competing interests exist.
Douglas Koshland

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States

For correspondence
koshland@berkeley.edu

Competing interests
The authors declare that no competing interests exist.

"This ORCID iD identifies the author of this article:" 0000-0003-3742-6294

Funding

Helen Hay Whitney Foundation

Siheng Xiang

National Institute of General Medical Sciences (1R35 GM-118189-01)

Douglas Koshland

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Copyright

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.