Cohesin helps mediate sister chromatid cohesion, chromosome condensation, DNA repair and transcription regulation. We exploited proximity-dependent labeling to define the in vivo interactions of cohesin domains with DNA or with other cohesin domains that lie within the same or in different cohesin complexes. Our results suggest both cohesin's head and hinge domains bind to DNA, and cohesin structure is dynamic with differential folding of its coiled coil regions to generate butterfly confirmations. This method also reveals that cohesins form ordered clusters on and off DNA. The levels of cohesin clusters and their distribution on chromosomes are cell cycle-regulated. Cohesin clustering is likely necessary for cohesion maintenance because clustering and maintenance uniquely require the same subset of cohesin domains and the auxiliary cohesin factor Pds5p. These conclusions provide important new mechanistic and biological insights into the architecture of the cohesin complex, cohesin-cohesin interactions, and cohesin's tethering and loop extruding activities.
- Siheng Xiang
- Douglas Koshland
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
- Adèle L Marston, University of Edinburgh, United Kingdom
- Received: August 18, 2020
- Accepted: February 16, 2021
- Accepted Manuscript published: February 17, 2021 (version 1)
© 2021, Xiang & Koshland
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