The connectome of the adult Drosophila mushroom body provides insights into function
- Howard Hughes Medical Institute, United States
- Columbia University, United States
- University of Cambridge, United Kingdom
- University of Oxford, United Kingdom
- MRC Laboratory of Molecular Biology, United Kingdom
- Cambridge University, United Kingdom
Abstract
Making inferences about the computations performed by neuronal circuits from synapse-level connectivity maps is an emerging opportunity in neuroscience. The mushroom body (MB) is well positioned for developing and testing such an approach due to its conserved neuronal architecture, recently completed dense connectome, and extensive prior experimental studies of its roles in learning, memory and activity regulation. Here we identify new components of the MB circuit in Drosophila, including extensive visual input and MB output neurons (MBONs) with direct connections to descending neurons. We find unexpected structure in sensory inputs, in the transfer of information about different sensory modalities to MBONs, and in the modulation of that transfer by dopaminergic neurons (DANs). We provide insights into the circuitry used to integrate MB outputs, connectivity between the MB and the central complex and inputs to DANs, including feedback from MBONs. Our results provide a foundation for further theoretical and experimental work.
Data availability
All the primary data used in this study is freely available through a public server (neuprint.janelia.org). All the underlying data behind that server is open source (CC-BY). There is no institutional resource for hosting connectome data. We commit to keeping this available for at least 10 years, and provide procedures where users can copy any or all of it to their own computer. Login is via any Google account; users who wish to remain anonymous can create a separate account for access purposes only.
Article and author information
Author details
Markus William Pleijzier
Gregory SXE Jefferis
Ashok Litwin-Kumar
Funding
Howard Hughes Medical Institute (internal funding)
- Marisa Dreher
- Aljoscha Nern
- Shin-ya Takemura
- Nils Eckstein
- Audrey Francis
- Ruchi Parekh
- Louis K Scheffer
- Yoshi Aso
- Gerald M Rubin
Wellcome Trust (203261/Z/16/Z)
- Feng Li
- Elizabeth C Marin
- Nils Otto
- Georgia Dempsey
- Ildiko Stark
- Philipp Schlegel
- Tansy Yang
- Amalia Braun
- Marta Costa
- Gregory SXE Jefferis
- Scott Waddell
- Gerald M Rubin
Wellcome Trust (200846/Z/16/Z)
- Scott Waddell
National Science Foundation (NeuroNex DBI-1707398)
- Jack W Lindsey
- Larry F Abbott
- Ashok Litwin-Kumar
Department of Energy (DE-SC0020347)
- Jack W Lindsey
Medical Research Council (MC-U105188491)
- Alexander S Bates
- Markus William Pleijzier
- Philipp Schlegel
- Gregory SXE Jefferis
Simons Foundation (SCGB)
- Jack W Lindsey
- Yoshi Aso
- Larry F Abbott
- Ashok Litwin-Kumar
- Gerald M Rubin
Burroughs Wellcome Fund (1017109)
- Ashok Litwin-Kumar
National Institutes of Health (R01EB029858)
- Ashok Litwin-Kumar
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2020, Li et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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The connectome of the adult Drosophila mushroom body provides insights into function
eLife 9:e62576.
https://doi.org/10.7554/eLife.62576
