Cystic proliferation of germline stem cells is necessary to reproductive success and normal mating behavior in medaka
- Instituto Tecnológico de Chascomús, INTECH (CONICET-UNSAM), Argentina
- Institute of Biosciences, São Paulo State University (UNESP), Brazil
- Salmonid Experimental Station at Campos do Jordão, Brazil
Abstract
The production of an adequate number of gametes is necessary for normal reproduction, for which the regulation of proliferation from early gonadal development to adulthood is key in both sexes. Cystic proliferation of germline stem cells is an especially important step prior to the beginning of meiosis; however, the molecular regulators of this proliferation remain elusive in vertebrates. Here, we report that ndrg1b is an important regulator of cystic proliferation in medaka. We generated mutants of ndrg1b that led to a disruption of germ cells cystic proliferation. This loss of cystic proliferation was observed from embryogenic to adult stages, impacting the success of gamete production and reproductive parameters such as spawning and fertilization. Interestingly, the depletion of cystic proliferation also impacted male sexual behavior, with a decrease of mating vigor. These data illustrate why it is also necessary to consider gamete production capacity in order to analyze reproductive behavior.
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All data generated or analysed during this study are included in the manuscript and supporting files.
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Funding
Agencia Nacional de Promoción Científica y Tecnológica (Grant 0366/12 and 2501/15)
- Luisa F Arias Padilla
Fundação de Amparo à Pesquisa do Estado de São Paulo (14/07620-7 and 18/10265-5)
- Rafael H Nóbrega
CONICET and São Paulo Research Foundation (International Cooperation Grant D 2979/16)
- Ivana F Rosa
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2021, Arias Padilla et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Cystic proliferation of germline stem cells is necessary to reproductive success and normal mating behavior in medaka
eLife 10:e62757.
https://doi.org/10.7554/eLife.62757
