(A) Increased Pto DC3000 vascular propagation in the two independent ros1 mutant alleles, ros1-3 and ros1-4. Secondary veins of 5-week-old Col-0, ros1-3 and ros1-4 mutants were inoculated with a …
Original data of bacterial propagation assays for Figure 1A,C,D, and F.
(A) RMG1 mRNA levels in Col-0 and the two rmg1 mutant alleles, namely rmg1-1 and rmg1-2, were monitored by RT-qPCR at 6 hr after syringe-infiltration of mock (water) or 1 μM of flg22 peptide. (B) RMG…
Original qRT-PCR data for Figure 2A and F, and bacterial propagation data for Figure 2B and C.
The T-DNA insertions SALK_007034 (rmg1-1 allele) and SALK_023944 (rmg1-2 allele) are located in the second exon and in the first intron of RMG1 (AT4G11170), respectively.
IGV snapshots showing mRNA levels (mRNA-seq) after syringe-infiltration of mock (water) or 1 μM of flg22 peptide in 5-week-old rosette leaves of Col-0 and ros1-3, and cytosine methylation levels …
Second biological replicate of RT-qPCR analyses depicting RMG1, FRK1, RLP43, and RBA1 mRNA levels in 5-week-old rosette leaves of Col-0, ros1-3, dcl23, and ros1dcl23 treated with either mock (water) …
Original qRT-PCR data for Figure 2—figure supplement 3.
(A) Proportion of flg22-responsive genes that are regulated by ROS1. One hundred and two flg22-responsive genes that are ‘less-induced’ and 115 flg22-responsive genes that are ‘less-repressed’ in ros…
Original qRT-PCR data for Figure 3G.
Original transcriptomic data used for the heatmap presented in Figure 3B.
(A) Heatmap depicting the relative expression of the 102 less-induced genes in Col-0 and ros1-3 mutants upon 6 hr of mock or flg22 treatments. AGI numbers and gene names are shown for all individual …
(A) Heatmaps depicting 23nt- and 24nt-long siRNA levels at the 102 less-induced genes (left panel) and at the 2943 genes presenting hyperDMR in ros1-3 (right panel) on replicate 1. Global siRNA …
(A) Snapshot representing levels of DNA methylation and siRNA species classified per size at the RLP43 promoter, in 5-week-old rosette leaves of untreated Col-0, ros1-3, and ros1dcl23. (B) Snapshot …
(A) A subgroup of the ROS1 targets exhibits an over-representation of WRKY DNA binding at the promoter regions corresponding to the hyperDMRs that were retrieved in ros1-3. GAT analysis performed on …
Original qRT-PCR data of GUS transcript quantification for Figure 4E.
(A) GAT analysis performed on publicly available DNA affinity purification sequencing (DAP-seq) data (O'Malley et al., 2016) for WRKY TFs at the 26 stringent ROS1 primary targets. This analysis was …
(A) GAT analysis performed on publicly available DNA affinity purification sequencing (DAP-seq) data (O'Malley et al., 2016) for WRKY TFs at the 28 stringent less-repressed ROS1 targets, from which …
Snapshots representing, from top to bottom, RNA-seq, BS-seq, and DNA affinity purification sequencing (DAP-seq) data sets at the RMG1 locus (left panel) with an enlargement surrounding the hyperDMR …
Snapshots depicting RNA-seq (rep1), BS-seq (rep1 and rep2), and the position of WRKY-binding sites observed in the DNA affinity purification sequencing (DAP-seq) data (O'Malley et al., 2016) in …
Snapshots representing mRNA-seq and BS-seq of Col-0 and ros1 untreated rosette leaves and ChIP-seq data for wrky18 WRKY18-HA and wrky40 WRKY40-HA seedlings subjected to either mock (medium without …
(A) DAP-seq data from O'Malley et al., 2016 showing that WRKY family members are generally sensitive to DNA methylation. Graph representing ratio of binding capacity in DAP versus ampDAP data at …
Original DAP-qPCR data for Figure 5B.
The ability of WRKY18 and WRKY40 to bind DNA corresponding to the demethylated region of the RLP43 promoter is abolished in the ros1-3 mutant background. Second biological replicate of the DAP-qPCR …
Original DAP-qPCR data for Figure 5—figure supplement 1.
(A) Scheme depicting the chimeric inverted repeat (IR) construct designed to simultaneously direct DNA remethylation at the RMG1 and RLP43 promoter regions. The IR-RMG1p/RLP43p contains the …
Original McrBC-qPCR, qRT-PCR, and bacterial propagation data for Figure 6B–E.
(A) Accumulation of siRNAs at RLP43 and RMG1 promoters in IR-RMG1p/RLP43p lines was detected by low molecular weight northern blot analysis. U6 was used as a loading control. (B) Result from another …
Original qRT-PCR data for Figure 6—figure supplement 1.
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Gene (Arabidopsis thaliana) | ROS1 | Arabidopsis.org | AT2G36490 | |
Gene (Arabidopsis thaliana) | DCL2 | Arabidopsis.org | AT3G03300 | |
Gene (Arabidopsis thaliana) | DCL3 | Arabidopsis.org | AT3G43920 | |
Gene (Arabidopsis thaliana) | RMG1 | Arabidopsis.org | AT4G11170 | |
Gene (Arabidopsis thaliana) | RLP43 | Arabidopsis.org | AT3G28890 | |
Strain, strain background (Pseudomonas syringae pv. tomato) | Pto DC3000-GFP | Gift from Pr. Sheng Yang He (Duke University, US). | ||
Genetic reagent (Arabidopsis thaliana) | ros1-3 | Penterman et al., 2007 | ||
Genetic reagent (Arabidopsis thaliana) | ros1-4 | Nottingham Arabidopsis stock center (NASC) | N682295 | |
Genetic reagent (Arabidopsis thaliana) | dcl2-1 dcl3-1 | Xie et al., 2004 | ||
Genetic reagent (Arabidopsis thaliana) | ros1-3 dcl2-1 dcl3-1 | This study | ||
Genetic reagent (Arabidopsis thaliana) | rmg1-1 | Nottingham Arabidopsis stock center (NASC) | N678063 | |
Genetic reagent (Arabidopsis thaliana) | rmg1-2 | Nottingham Arabidopsis stock center (NASC) | N674117 | |
Commercial assay or kit | MagneGST Pull-Down System | Promega | V8870 | |
Commercial assay or kit | McrBC | New England Biolab (NEB) | M0272 |
DMRs position of 102 less-induced and 115 less-repressed ROS1 targets.
Primers used in this study.