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Control of feeding by Piezo-mediated gut mechanosensation in Drosophila

  1. Soohong Min
  2. Yangkyun Oh
  3. Pushpa Verma
  4. Samuel C Whitehead
  5. Nilay Yapici
  6. David Van Vactor
  7. Greg SB Suh
  8. Stephen Liberles  Is a corresponding author
  1. Howard Hughes Medical Institute, Harvard Medical School, Department of Cell Biology, United States
  2. Skirball Institute, NYU School of Medicine, United States
  3. Harvard Medical School, Department of Cell Biology, United States
  4. Department of Physics, Cornell University, United States
  5. Department of Neurobiology and Behavior, Cornell University, United States
  6. KAIST, Department of Biological Sciences, Republic of Korea
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Cite this article as: eLife 2021;10:e63049 doi: 10.7554/eLife.63049

Abstract

Across animal species, meals are terminated after ingestion of large food volumes, yet underlying mechanosensory receptors have so far remained elusive. Here, we identify an essential role for Drosophila Piezo in volume-based control of meal size. We discover a rare population of fly neurons that express Piezo, innervate the anterior gut and crop (a food reservoir organ), and respond to tissue distension in a Piezo-dependent manner. Activating Piezo neurons decreases appetite, while Piezo knockout and Piezo neuron silencing cause gut bloating and increase both food consumption and body weight. These studies reveal that disrupting gut distension receptors changes feeding patterns and identify a key role for Drosophila Piezo in internal organ mechanosensation.

Introduction

Mechanosensory neurons detect a variety of environmental forces that we can touch or hear, as well as internal forces from organs and tissues that control physiological homeostasis (Abraira and Ginty, 2013; Ranade et al., 2015; Umans and Liberles, 2018). In many species, specialized mechanosensory neurons innervate the gastrointestinal tract and are activated by tissue distension associated with consuming a large meal (Williams et al., 2016; Zagorodnyuk et al., 2001). Gut mechanosensation may provide an evolutionarily conserved signal for meal termination as gut distension inhibits feeding in many species and evokes the sensation of fullness in humans (Phillips and Powley, 1996; Rolls et al., 1998). However, how gut distension receptors contribute to long-term control of digestive physiology and behavior is unclear as tools for selective pathway manipulation are lacking. Identifying neuronal mechanisms involved in detecting the volume of ingested food would provide basic insights into this fundamental mechanosensory process, and in humans, perhaps clinical targets for feeding and metabolic disorders.

Here, we investigated the roles and mechanisms of food volume sensation in the fruit fly Drosophila melanogaster. Volumetric control of feeding was classically studied in a larger related insect, the blowfly, with relevant mechanosensory hotspots identified in the foregut and crop, an analog of the stomach (Dethier and Gelperin, 1967; Gelperin, 1967). In Drosophila, chemosensory neurons detect nutrients in the periphery and brain to control appetite, with some neurons positively reinforcing feeding during starvation conditions (Bjordal et al., 2014; Dus et al., 2015; Miyamoto et al., 2012). In contrast, the importance of gut mechanosensation in Drosophila feeding control and digestive physiology has not been similarly investigated; mechanosensory neurons of the gustatory system sense food texture and modulate ingestion (Sánchez-Alcañiz et al., 2017; Zhang et al., 2016), and other mechanosensory neurons in the posterior gut control defecation and food intake (Olds and Xu, 2014; Zhang et al., 2014). In contrast, food storage during a meal occurs primarily in the anterior gut (Lemaitre and Miguel-Aliaga, 2013; Stoffolano and Haselton, 2013). Enteric neurons of the hypocerebral ganglion innervate the fly crop, foregut, and anterior midgut, and lesioning of the recurrent nerve (which contains neurons of the hypocerebral ganglion) in Drosophila and blowfly increases feeding duration (Dethier and Gelperin, 1967; Gelperin, 1967; Pool et al., 2014). Together, these prior studies raise the possibility that a subpopulation of enteric neurons in Drosophila could be specialized to sense meal-associated gut distension.

Results and discussion

Piezo-expressing enteric neurons innervate the gastrointestinal tract

To explore whether food volume sensation occurs in Drosophila and to investigate underlying mechanisms, we first asked whether neurons expressing various mechanosensory ion channels innervated the anterior gut. Several mechanosensitive ion channels have been reported in Drosophila, including TRP channels (Nompc, Nanchung, and Inactive), the degenerin/epithelial sodium channel Pickpocket (Ppk), transmembrane channel-like (Tmc) protein, and Piezo (Coste et al., 2012; Montell, 2005; Zhang et al., 2016; Zhong et al., 2010). We obtained Gal4 driver lines that mark neurons containing mechanoreceptor proteins or related family members, induced expression of membrane-tethered CD8-Green Fluorescent Protein (GFP) or dendritically targeted DenMark fluorescent reporters, and visualized neuronal innervation of the anterior gut. We observed a small group of Piezo-expressing enteric neurons located in the hypocerebral ganglion (~5–6 neurons per fly), and a dense network of Piezo fibers throughout the crop and anterior midgut (Figure 1A and B). Hypocerebral ganglion neurons were similarly labeled and anterior gut innervation similarly observed in three independent Piezo-Gal4 driver lines (Figure 1—figure supplement 1A), but not in other Gal4 lines analyzed. We noted Nanchung expression in some epithelial cells of the crop duct, but not in crop-innervating neurons. The hypocerebral ganglion and adjacent corpora cardiaca together contain ~35 neurons per fly based on Elav immunohistochemistry, and Piezo neurons therein were distinct from other neurons that expressed the fructose receptor Gr43a (~5 neurons per fly) or the glucagon analog adipokinetic hormone (Akh, ~20 neurons per fly) (Figure 1C, D, Figure 1—figure supplement 1B). Piezo neurites formed a muscle-associated lattice in the gut, and ascending axons contributed to the recurrent nerve (Figure 1E, F). Using a genetic approach involving the MultiColor FlpOut system (Nern et al., 2015) for sparse labeling of Piezo cells, flies were obtained with reporter expression in one or a few hypocerebral ganglion neurons but not in brain structures such as the pars intercerebralis; in these flies, separate Piezo neurons were observed to innervate the crop and/or anterior midgut (Figure 1—figure supplement 1C). Drosophila Piezo was previously shown to confer mechanically activated currents when expressed in human cells and to mediate mechanical nociception (Coste et al., 2012; Kim et al., 2012). Furthermore, vertebrate Piezo homologs play diverse mechanosensory roles, including in internal sensation of airway volume and blood pressure (Min et al., 2019; Nonomura et al., 2017; Zeng et al., 2018). We hypothesized that Drosophila enteric neurons that express Piezo and innervate the anterior gut might mediate volumetric control of appetite.

Figure 1 with 1 supplement see all
Piezo neurons innervate the gastrointestinal tract.

(A) Wholemount image of the digestive tract from a Piezo-Gal4 (59266); UAS-DenMark fly visualized with immunofluorescence for DenMark (red, anti-Red Fluorescent Protein or RFP) and a fluorescent Phalloidin conjugate (blue) to label visceral muscle. HCG: hypocerebral ganglion, scale bar 100 μm. (B) Immunofluorescence for RFP (red) and Elav (blue) in the HCG from a Piezo-Gal4; UAS-CD8RFP fly, scale bar 10 μm. (C) Immunofluorescence for GFP (green) and Akh (magenta) in the corpora cardiaca (CC) and HCG from a Piezo-Gal4; UAS-CD8GFP fly, scale bar 10 μm. (D) Native GFP and RFP fluorescence from the HCG of a Piezo-Gal4; UAS-CD8RFP; Gr43a-LexA; LexAop-CD8GFP fly, scale bar 10 μm. (E) Image of the recurrent nerve (arrows) labeled by native RFP fluorescence in a Piezo-Gal4; UAS-CD8RFP fly and a fluorescent Phalloidin conjugate (blue), scale bar 10 μm. (F) The anterior midgut (left) and crop (right) of a Piezo-Gal4; UAS-DenMark fly visualized by immunofluorescence for DenMark (green) and a fluorescent Phalloidin conjugate (magenta), scale bar 50 μm. See Figure 1—figure supplement 1 and source data.

Piezo neurons control feeding behavior

To explore this model, we activated and silenced Piezo neurons using genetic approaches and monitored feeding behavior. We expressed temperature-sensitive Shibire (Shits) that blocks synaptic transmission at non-permissive temperatures (>32°C) in Piezo neurons using three independent Piezo-Gal4 drivers (Piezo>Shits). Piezo>Shits flies were reared at a permissive temperature (18°C) and later tested for physiological and behavioral changes at 32°C. To measure feeding behavior, flies were fasted for 24 hr, and then given brief access (30 min) to food containing a dye for visualization and quantification of ingestion (Figure 2A). Piezo>Shits flies from all three genotypes fed ravenously, and histological examination of the gastrointestinal tract showed gut bloating with increased crop size (Figure 2B). For comparison, genetic silencing of other gut-innervating neurons labeled in GMR51F12-Gal4 flies (Figure 2—figure supplement 1) did not impact appetite or cause crop distension. These findings indicate that disrupting Piezo neurons compromises gut volume homeostasis and associated control of feeding.

Figure 2 with 3 supplements see all
Piezo neurons control feeding behavior.

(A) Depiction of the colorimetric feeding assay. (B) Fasted flies with Shibire alleles indicated were given brief access (30 min) to dye-labeled food at 32°C, and feeding indices and crop sizes were calculated. n (left to right) (feeding index): 16, 11, 13, 10, and 10 trials involving 12 flies per trial. n (crop size): 13, 9, 11, 9, and 9 flies, mean ± SEM, ***p<0.0005, **p<0.005, *p<0.05, ns: not significant by ANOVA Dunnett’s multiple comparison test. (C) Fasted flies with Trpa1 alleles indicated were given brief access (30 min) to dye-labeled food at 30°C, and feeding indices and crop sizes were calculated. n (left to right) (feeding index): 19, 20, 14, 10, and 13 trials involving 12 flies per trial. n (crop size): 12, 6, 11, 10, and 12 flies, mean ± SEM, ***p<0.0005, **p<0.005, ns: not significant by ANOVA Dunnett’s multiple comparison test. (D) Native RFP fluorescence in brain (top), ventral nerve cord (VNC, middle), and hypocerebral ganglion (HCG, bottom) of Piezo-Gal459266; UAS-CD8RFP flies with Gal80 alleles indicated, scale bar 100 μm (brain, VNC), 20 μm (HCG). (E) Fasted flies with Trpa1 alleles indicated were given brief access (30 min) to dye-labeled food at 30°C, and feeding indices were calculated. n (left to right): 15, 20, 14, and 15 trials involving 12 flies per trial, mean ± SEM, ***p<0.0005, **p<0.005, ns: not significant by ANOVA Dunnett’s multiple comparison test. See Figure 2—figure supplements 13 and source data.

To test the effects of activating Piezo neurons on food consumption, we drove expression of the temperature-regulated ion channel Trpa1 in Piezo neurons using Piezo-Gal4 lines (Piezo>Trpa1). Thermogenetic activation of Trpa1 in Piezo cells, achieved by transferring Piezo>Trpa1 flies from 18°C to 30°C, suppressed food intake after a 24-hr fast and also blocked meal-associated increases in crop volume, with similar results observed using three different Piezo-Gal4 drivers (Figure 2C). Since many cell types express Piezo (Kim et al., 2012), we next used approaches for intersectional genetics involving Gal80, a dominant suppressor of Gal4-mediated gene induction to restrict Trpa1 expression to fewer cells. First, we drove Gal80 expression broadly in neurons using Piezo>Trpa1; Elav-Gal80 flies and observed restoration of normal feeding behavior, indicating the relevant Piezo expression site to be neurons (Figure 2D, E). Among neurons, Piezo-Gal4 drove expression in various peripheral sensory neurons, the ventral nerve cord, brain, and hypocerebral neurons. Differential expression control could be partially achieved using a Cha-Gal80 driver, which silences Gal4-mediated expression in the ventral nerve cord and many central neurons, but not in gut-innervating hypocerebral neurons or a few cells of the proboscis, intestine, and brain (Figure 2D, Figure 2—figure supplement 2A). Thermogenetic experiments in Piezo>Trpa1; Cha-Gal80 flies also caused robust suppression of feeding behavior (Figure 2E). Intestinal cells are unlikely to contribute to feeding phenotypes in Piezo>Trpa1; Cha-Gal80 flies based on experiments involving Piezo>Trpa1; Elav-Gal80 flies; to provide additional evidence, we obtained Escargot-Gal4 flies in which Piezo-expressing intestinal stem cells (ISCs) are broadly marked (He et al., 2018) and found that thermogenetic activation of intestinal cells using Escargot-Gal4; UAS-Trpa1 flies also had no effect on feeding (Figure 2—figure supplement 2B, C). Piezo neurons expressing Dilp2 in the pars intercerebralis are also reported to innervate the crop and control feeding behavior (Wang et al., 2020), which potentially explain the significant differences we observe in feeding following thermogenetic activation experiments involving Piezo-Gal4; UAS-Trpa1 and Piezo-Gal4; UAS-Trpa1; Cha-Gal80 flies (Figure 2E). In control Piezo-Gal4; UAS-CD8RFP flies, we observed reporter expression per fly in 6.2 ± 0.5 hypocerebral neurons and 4.9 ± 1.0 pars intercerebralis neurons, 2.9 ± 0.7 of which express Dilp2. In Piezo-Gal4; UAS-CD8RFP; Cha-Gal80 flies, we observed reporter expression per fly in 5.2 ± 0.5 hypocerebral neurons and 1.1 ± 0.5 pars intercerebralis neuron, 0.4 ± 0.3 of which express Dilp2 (about half of flies had one co-labeled neuron and half had zero) (Figure 2—figure supplement 3A–C). In flies that lacked any reporter expression in pars intercerebralis Dilp2 neurons, we still observed labeled neurites in the anterior midgut and crop nerve, consistent with findings from stochastic labeling (Figure 1—figure supplement 1C) that neurons outside of the pars intercerebralis innervate these regions. Furthermore, Dilp2-Gal4 does not label Elav-marked hypocerebral neurons (Figure 2—figure supplement 3D). Additional studies are needed to distinguish the contributions of hypocerebral and pars intercerebralis Piezo neurons, with data so far suggesting that both subtypes of Piezo neurons contribute to feeding control.

Piezo enteric neurons respond to crop-distending stimuli

Next, we investigated the response properties of Piezo-expressing enteric neurons. We analyzed neuronal activity using a transcriptional reporter system involving CaLexA through which sustained neural activity drives expression of GFP (Masuyama et al., 2012). CaLexA reporter was expressed in Piezo neurons using Gal4 drivers, along with an orthogonal activity-independent CD8-RFP reporter for normalization. For validation and determination of response kinetics, Trpa1-induced activation of Piezo neurons increased CaLexA reporter levels gradually, with maximal induction by 24 hr (Figure 3—figure supplement 1A). First, we asked whether hypocerebral Piezo neurons, and for comparison hypocerebral Gr43a neurons that function as peripheral sugar sensors, changed activity with feeding state (Figure 3A, B). For both neuron types, we observed that CaLexA-driven GFP expression was low after a fast or in flies fed ad libitum, but was strikingly elevated when flies engorged themselves on a sucrose diet (Figure 3A, B, Figure 3—figure supplement 1B). Sucrose consumption could potentially stimulate both gut chemosensors and mechanosensors as an increase in crop volume was observed compared with flies fed ad libitum (Figure 3—figure supplement 1C). We next asked whether activity changes in enteric neurons depended on the content of ingested material. We compared CaLexA-mediated GFP expression levels in flies fed for 24 hr with (1) sucrose, (2) sucralose, a sweetener that lacks caloric value and stimulates peripheral gustatory receptors but not internal Gr43a neurons, (3) water alone after a period of water deprivation, or (4) water alone ad libitum. Flies extensively consumed sucrose, sucralose, and water when water-deprived, resulting in acute increases in crop volume that were not observed in flies given only water ad libitum (Figure 3C). Enteric Gr43a neurons displayed elevated levels of CaLexA-mediated GFP expression after engorgement on sucrose, which is converted into fructose and glucose, but not sucralose or water, consistent with a role for these neurons in sensing nutritional carbohydrates (Miyamoto and Amrein, 2014). In contrast, enteric Piezo neurons were activated more generally by sucrose, sucralose, and deprivation-induced water ingestion, but not in controls given only water ad libitum, with responses correlated to the extent of gut distension. The observation that Piezo neurons were similarly activated by water- and sucrose-induced gut distension indicated a sensory mechanism that does not require chemosensation of particular nutrients. Together, these findings suggest a model of two segregated sensory pathways through the hypocerebral ganglion, with Gr43a neurons responding to sugars and Piezo neurons responding to anterior gut mechanosensation.

Figure 3 with 1 supplement see all
Piezo mediates enteric neuron responses to crop-distending stimuli.

(A) Flies of genotypes indicated were provided solutions of (1) sucrose, (2) sucralose, (3) water alone after a period of water deprivation (water), or (4) water alone ad libitum for 24 hr (control). Representative images of native CaLexA-induced GFP reporter (green) and CD8RFP (red) fluorescence visualized in enteric Gr43a neurons (left), Piezo neurons (middle), or Piezo neurons lacking Piezo (right), scale bar 10 μm. (B) Quantification of CaLexA-induced GFP fluorescence in individual RFP-expressing neurons from flies in (A). n (from top to bottom): 59, 64, 43, and 67 Gr43a neurons from 13, 14, 9, and 15 flies; 61, 61, 59, and 66 Piezo neurons from 11, 11, 10, and 12 flies; 60, 60, 33, and 37 Piezo-null Piezo neurons from 11, 11, 5, and 6 flies, mean ± SEM, ***p<0.0001, ns: not significant by ANOVA Dunnett’s multiple comparison test. (C) Visualization of the crop from flies given stimuli indicated after 24 hr (sucrose, sucralose, control) or 15 min (water), scale bar 100 μm. See Figure 3—figure supplement 1 and source data.

Piezo knockout alters enteric neuron responses and fly feeding behavior

Next, we asked whether the Piezo receptor mediates neuronal responses of hypocerebral neurons. We obtained Piezo knockout flies and crossed them with flies harboring alleles, enabling the CaLexA reporter system in Piezo neurons (using Piezo-Gal459266 flies with the Piezo-Gal4 transgene remote from the endogenous Piezo locus). Remarkably, hypocerebral ganglion neurons marked in Piezo-Gal4 flies but lacking Piezo expression did not respond to engorgement by sucrose, sucralose, or water, even though the crops of Piezo knockout flies were distended (Figure 3A, B). (As shown below, the extent of distension is actually more pronounced in Piezo knockout flies, yet CaLexA-mediated responses were not observed.) A lack of neuronal responses in Piezo knockout flies is not due to gross deficits in the ability to produce reporter as Trpa1-mediated activation of Piezo neurons in Piezo knockout flies was sufficient to induce a CaLexA-mediated response (Figure 3—figure supplement 1D). Furthermore, Piezo neurons still innervated the anterior gut, suggesting that the deficit was not due to coarse developmental miswiring (Figure 3—figure supplement 1E). Instead, enteric neurons of Piezo knockout flies seemingly fail to respond to crop-distending stimuli due to a mechanosensory defect.

Next, we asked whether Piezo knockout flies display changes in behavior or physiology. We measured feeding behavior in Piezo knockout flies and, for comparison, isogenic w1118 flies. For synchronization, flies were fasted for 18 hr and then given ad libitum access to dye-labeled food for 30 min. Remarkably, Piezo knockout flies increased food intake and had visually observable crop distension (Figure 4A–C, Figure 4—figure supplement 2A). Moreover, Piezo knockout flies fed ad libitum on normal fly food for 5–7 days showed an increase in body weight compared to control flies (Figure 4D). Automated analysis of feeding patterns was performed involving an EXPRESSO platform (Yapici et al., 2016), and Piezo knockout flies displayed an increase in food intake and feeding bout duration but a similar frequency of feeding bout initiation (Figure 4E). Abnormal gut distension and feeding behavior were rescued by exogenous expression of Piezo-GFP in Piezo knockout neurons driven by Piezo-Gal4 (Figure 4F, Figure 4—figure supplement 1A). Unlike Drop-dead knockout flies that have an enlarged crop due to defective food passage into the intestine (Peller et al., 2009), Piezo knockout flies have normal food transit, a normal lifespan, and increased defecation rates, presumably due to increased feeding (Figure 4—figure supplement 1B–E). Other than food-induced distension, the anatomy of the crop appeared normal in Piezo knockout flies as visualized by histology of crop muscle, analysis of cell density, and volume measurements during starvation (Figure 4—figure supplement 1F–H). As mentioned above, knockout of Piezo does not impact the extent of gut innervation (Figure 3—figure supplement 1E); furthermore, thermogenetic Trpa1-mediated activation of Piezo neurons in Piezo knockout flies suppressed feeding behavior (Figure 4—figure supplement 1I), indicating that neural circuits downstream of enteric Piezo neurons were intact and remained capable of eliciting a behavioral response after Piezo knockout. Piezo also functions to guide the differentiation of gut enteroendocrine cells from mechanosensitive ISCs (He et al., 2018); however, selectively restoring Piezo expression in ISCs using Escargot-Gal4 (Esg-Gal4) did not rescue crop volume and feeding phenotypes (Figure 4—figure supplement 1J). We also note that while the crops of Piezo knockout flies are distended, the flies eventually stop eating (although abdomen bursting does rarely occur, Figure 4—figure supplement 2B), suggesting either a physical limitation to ingestion or eventual engagement of a secondary satiety pathway, perhaps through nutrient sensors or posterior gut mechanoreceptors. Taken together, our data indicate a role for Piezo in sensing anterior gut distension, and that disrupting the function of Piezo neurons, or Piezo itself, causes substantial changes to gut physiology and feeding behavior.

Figure 4 with 2 supplements see all
Piezo knockout alters fly feeding behavior.

(A) Fasted wild-type (WT) and Piezo knockout (KO) female flies were given brief access (30 min) to dye-colored food and imaged, scale bar 0.5 mm. (B) Representative images of the crop (arrow) in WT and Piezo KO flies, scale bar 100 μm, (C) Calculated feeding indices (left) and crop sizes (right) from flies in (A). n (feeding index: 17 trials involving 204 flies), n (crop size): 14 flies, mean ± SEM, ***p<0.0001 by unpaired t-test. (D) Body weights of WT and Piezo KO flies fed regular food ad libitum. n (left to right): 32, 34, 33, and 31 trials involving three flies per trial, mean ± SEM, ***p<0.0001 by unpaired t-test. (E) Feeding parameters of fasted WT and Piezo KO male flies were analyzed using the EXPRESSO assay for 30 min after food introduction to determine overall food consumption, feeding duration per bout, and the number of bouts. n: 21 (WT), 22 (PIEZO KO) flies, mean ± SEM, ***p<0.0005, ns: not significant by unpaired t-test. (F) Calculated feeding indices (left) and crop sizes (right) from Piezo rescue and control flies indicated. n (left to right) (feeding index): 29, 22, 30, 22, and 13 trials involving 12 flies per trial. n (crop size): 13, 13, 13, 18, and 16 flies, mean ± SEM, ***p<0.0005, **p<0.005 by ANOVA Dunnett’s multiple comparison test, ns: not significant by unpaired t test. See Figure 4—figure supplements 1 and 2 and source data.

Food-induced gut distension is thought to be an evolutionarily conserved signal for meal termination, yet underlying mechanisms and sensory receptors have long remained mysterious. Furthermore, whether food volume sensors are required for normal feeding control has remained unknown as tools for selective loss of function were not available without knowing the underlying sensory mechanisms. Here, we reveal a role for Drosophila Piezo in neurons that innervate the anterior gut and sense the size of a meal. Disrupting this pathway increases food consumption and body weight, and causes swelling of the gastrointestinal tract. These studies demonstrate that anterior gut mechanosensation contributes to the complex calculus that underlies the decision to eat, and provide a foundation for the comparative physiology and evolution of feeding control. Moreover, understanding related pathways in humans may enable new therapies for treating obesity and other food consumption disorders.

Materials and methods

Key resources table
Reagent type
(species) or
resource
DesignationSource or
reference
IdentifiersAdditional
information
Genetic reagent (Drosophila melanogaster)Piezo-Gal4Bloomington Drosophila Stock CenterBDSC: 59266; RRID:BDSC_59266
Genetic reagent (D. melanogaster)Piezo(KI)-Gal4He et al., 2018PMID:29414942
Genetic reagent (D. melanogaster)Piezo(gene-trap)-Gal4Bloomington Drosophila Stock CenterBDSC: 76658
RRID:BDSC_76658
Genetic reagent (D. melanogaster)Piezo KOBloomington Drosophila Stock CenterBDSC: 58770;
RRID:BDSC_58770
Isogenized with w1118
Genetic reagent (D. melanogaster)UAS-GFP-PiezoBloomington Drosophila Stock CenterBDSC: 58773;
RRID:BDSC_58773
Genetic reagent (D. melanogaster)UAS-CD8RFPBloomington Drosophila Stock CenterBDSC: 32218; RRID:BDSC_32218
Genetic reagent (D. melanogaster)Hs-Flp, UAS-MCFOBloomington Drosophila Stock CenterBDSC: 64085; RRID:BDSC_64085
Genetic reagent (D. melanogaster)UAS-CD8GFPBloomington Drosophila Stock CenterBDSC: 5137;
RRID:BDSC_5137
Genetic reagent (D. melanogaster)UAS-Trpa1Bloomington Drosophila Stock CenterBDSC: 26263; RRID:BDSC_26263
Genetic reagent (D. melanogaster)UAS-CaLexABloomington Drosophila Stock CenterBDSC: 66542; RRID:BDSC_66542
Genetic reagent (D. melanogaster)Nanchung-Gal4Bloomington Drosophila
Stock Center
BDSC: 24903; RRID:BDSC_24903
Genetic reagent (D. melanogaster)Inactive-Gal4Bloomington Drosophila Stock CenterBDSC: 36360; RRID:BDSC_36360
Genetic reagent (D. melanogaster)Painless-Gal4Bloomington Drosophila Stock CenterBDSC: 27894; RRID:BDSC_27894
Genetic reagent (D. melanogaster)Tmc-Gal4Zhang et al., 2016PMID:27478019
Genetic reagent (D. melanogaster)Gr43a-Gal4Miyamoto et al., 2012PMID:23178127
Genetic reagent (D. melanogaster)Gr43a-LexAFujii et al., 2015PMID:25702577
Genetic reagent (D. melanogaster)UAS-DenMarkBloomington Drosophila Stock CenterBDSC: 33061; RRID:BDSC_33061
Genetic reagent (D. melanogaster)UAS-DenMarkBloomington Drosophila Stock CenterBDSC: 33062; RRID:BDSC_33062
Genetic reagent (D. melanogaster)Trp-Gal4Bloomington Drosophila Stock CenterBDSC: 36359;
RRID:BDSC_36359
Genetic reagent (D. melanogaster)Nompc-Gal4Bloomington Drosophila Stock CenterBDSC: 36360; RRID:BDSC_36360
Genetic reagent (D. melanogaster)Drop-dead KOBloomington Drosophila Stock CenterBDSC: 36360; RRID:BDSC_36360
Genetic reagent (D. melanogaster) w1118Bloomington Drosophila Stock CenterBDSC: 3605;
RRID:BDSC_3605
Genetic reagent (D. melanogaster)Trpa1-Gal4Bloomington Drosophila Stock CenterBDSC: 36362;
RRID:BDSC_36362
Genetic reagent (D. melanogaster)Ppk-Gal4Bloomington Drosophila Stock CenterBDSC: 32078;
RRID:BDSC_32078
Genetic reagent (D. melanogaster)GMR51F12-Gal4Bloomington Drosophila Stock CenterBDSC: 58685;
RRID:BDSC_58685
Genetic reagent (D. melanogaster)Cha-Gal80Sakai et al., 2009PMID:19531155
Genetic reagent (D. melanogaster)UAS-ShibiretsKitamoto, 2001PMID:11291099
Genetic reagent (D. melanogaster)Escargot-Gal4Hayashi et al., 2002PMID:12324948
Genetic reagent (D. melanogaster)Elav-Gal80Yang et al., 2009PMID:19249273
AntibodyAnti-Dilp2;
rabbit polyclonal
Veenstra Jan (University of Bordeaux, France)(1:200)
AntibodyAnti-GFP; chicken polyclonalThermo Fisher ScientificThermo Fisher Scientific Cat# A10262;
RRID:AB_2534023
(1:200)
AntibodyAnti-RFP; rabbit polyclonalRocklandRockland Cat# 600-401-379;
RRID:AB_2209751
(1:200)
AntibodyAnti-Elav;
mouse monoclonal
Developmental Studies Hydridoma BankDSHB Cat# Elav-9F8A9;
RRID:AB_528217
(1:200)
AntibodyAnti-Akh;
rabbit polyclonal
KerafastKerafast Cat# EGA261(1:200)
AntibodyAnti-Flag;
Rat monoclonal
Novus BiologicalsNovus Cat# NBP1-06712SS;
RRID:AB_1625982
(1:200)
AntibodyAnti-HA; Rabbit monoclonalCell Signaling TechnologyCell Signaling Technology Cat# 3724S;
RRID:AB_1549585
(1:200)
AntibodyAnti-V5; Mouse monoclonalBio-RadBio-Rad Cat# MCA2894D549GA
RRID:AB_10845946
(1:200)
AntibodyAlexa Fluor-488;
Chicken polyclonal
Jackson ImmunoResearchJackson ImmunoResearch Cat# 703-545-155;
RRID:AB_2340375
(1:400)
AntibodyAlexa Fluor-488;
Rabbit polyclonal
Jackson ImmunoResearchJackson ImmunoResearch Cat# 711-545-152;
RRID:AB_2313584
(1:400)
AntibodyCy3-AffiniPure;
Rabbit polyclonal
Jackson ImmunoResearchJackson ImmunoResearch Cat# 711-165-152;
RRID:AB_2307443
(1:400)
AntibodyAlexa Fluor 647;
Rabbit polyclonal
Jackson ImmunoResearchJackson ImmunoResearch Cat# 711-605-152;
RRID:AB_2492288
(1:400)
AntibodyAlexa Fluor 647;
Mouse polyclonal
Jackson ImmunoResearchJackson ImmunoResearch Cat# 715-605-150;
RRID:AB_2340862
(1:400)
AntibodyAlexa Fluor 488;
Mouse polyclonal
Jackson ImmunoResearchJackson ImmunoResearch Cat# 715-545-150;
RRID:AB_2340846
(1:400)
AntibodyAlexa Fluor 488;
Rat polyclonal
Jackson ImmunoResearchJackson ImmunoResearch Cat# 712-545-153; RRID:AB_2340684(1:400)
Chemical compound, drugNormal goat serumJackson ImmunoResearchJackson ImmunoResearch Cat# 005-000-121;
RRID:AB_2336990
(5%)
Chemical compound, drugFluoromount-GSouthern Biotech0100-01
Chemical compound, drugPhalloidin-FITCSigmaP5282-1MG(1:400)
Chemical compound, drugPhalloidin-TRITCSigmaP1951-1MG(1:400)
Chemical compound, drugTO-PRO-3ThermoFisherT3605(1:400)
Chemical compound, drugGreen food dyeAmazonAmazon standard identification number (ASIN): B0055AFE5GManufacturer: McCormick
Software, algorithmPrism 8GraphPadRRID:SCR_002798
Software, algorithmFijiSchindelin et al., Nature Methods, 2012PMID:22743772https://imagej.net/Fiji
Software, algorithmPython-based custom data analysis code used for EXPRESSO assaySamuel C. Whitehead, 2021, PiezoPaperExpressoCodehttps://github.com/scw97/PiezoPaperExpressoCode; Min, 2021; copy archived at swh:1:rev:bd8a58fa0e4f796e2ed0b72fe807862305b84b6b
OtherConfocal microscopeLeicaLeica SP5

Flies

Fly stocks were maintained on a regular cornmeal agar diet (Harvard Exelixis facility) at 25°C, with mating and collection performed under CO2 anesthesia. For Piezo knockout studies, Piezo knockout flies were isogenized by outcrossing five times into a wild-type w1118 isogenic background. We obtained Piezo knockout, knock-in (KI) Piezo-Gal4 and UAS-Piezo-GFP flies (Norbert Perrimon), Tmc-Gal4 (Craig Montell), knock-in Gr43a-LexA and knock-in Gr43a-Gal4 (Hubert Amrein), and from Bloomington Drosophila Stock Center Piezo-Gal4 (BDSC# 59266), Recombinase-Mediated Cassette Exchange (RMCE) gene-trap Piezo-Gal4 (BDSC# 76658), UAS-CD8GFP (BDSC# 5137), UAS-CD8RFP (BDSC# 32218), UAS-Trpa1 (BDSC# 26263), Cha-Gal80 (BDSC# 60321), UAS-CaLexA (BDSC# 66542), Nanchung-Gal4 (BDSC# 24903), Inactive-Gal4 (BDSC# 36360), Painless-Gal4 (BDSC# 27894), Trp-Gal4 (BDSC# 36359), Trpa1-Gal4 (BDSC# 36362), Nompc-Gal4 (BDSC# 36361), Ppk-Gal4 (BDSC# 32078), UAS-DenMark (BDSC# 33061 and 33062), Drop-dead KO (BDSC# 24901), w1118 (BDSC# 3605), GMR51F12-Gal4 (BDSC# 58685), and Hs-Flp; UAS-MCFO (BDSC# 64085). Escargot-Gal4, Cha-Gal80, Elav-Gal80, UAS-Shibirets, and Dilp2-Gal4 were as published (Hayashi et al., 2002; Ikeya et al., 2002; Kitamoto, 2001; Sakai et al., 2009; Yang et al., 2009).

Feeding analysis

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Acute feeding assays were performed as previously described with modifications (Albin et al., 2015; Min et al., 2016). Twelve adult female flies were collected upon eclosion and housed in a vial with for 5–7 days. Prior to testing, baseline hunger was synchronized by starving flies for 15–18 hr in a vial containing only on a dampened kimwipe section. The surface of regular fly food (typically ~16.25 ml per 50 ml vial) was dyed with green food coloring (McCormick, 70 µl dye per vial) and dried (24 hr). For testing, starved flies were transferred to vials containing dyed food for 30 min. Trials were ended by cooling the vials on ice, and a feeding index was scored as described below (see quantification). For thermogenetic experiments, flies expressing Trpa1 or Shibire were maintained and starved at 18°C prior to testing. Ten minutes prior to testing, starved flies and dye-labeled food were pre-warmed to 30°C or 32°C for experiments with either Trpa1 or Shibire, and then tested as above. Feeding behavior was scored by visual inspection of ingested dye with scores given from 0 to 5 based on dye intensity, as reported previously (Albin et al., 2015; Min et al., 2016). A feeding index was expressed by averaging the feeding scores for all flies per vial (~12 flies). For automated analysis of feeding patterns, fasted male flies (3–5 days old) were individually introduced into chambers connected to an EXPRESSO machine (http://public.iorodeo.com/docs/expresso/hardware_design_files.html) and feeding bouts were analyzed using EXPRESSO acquisition software (http://public.iorodeo.com/docs/expresso/device_software.html). Briefly, flies were given access (30 min) to a 200 mM sucrose solution through a capillary, and capillary fluid volume was measured over time using the EXPRESSO instrument. Total food consumption, feeding duration, feeding bout numbers, and feeding latency were then calculated using a Python-based custom data analysis code available at https://github.com/scw97/PiezoPaperExpressoCode.

Chronic studies of body weight, intestinal transit, fecal rate, and lifespan

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Chronic studies were performed on 5–7-day-old male and female flies fed ad libitum with regular fly food. Flies were anesthetized (ice, 10 min) and weighed in groups of three in a 1.5-ml Eppendorf tube, with body weight expressed as the average weight per group of three. Lifespan was analyzed for a group of 12 flies by counting the number of surviving flies each day. Fecal rates were measured after feeding flies dye-colored food (dye-colored food is described above) for 1 hr, with visual inspection of abdominal dye to ensure ingestion. Flies were transferred to an empty vial containing a 1 × 1 cm filter paper floor for 30 min, and dye-labeled fecal spots on the filter paper were counted. For analysis of fecal deposition, individual data points reflect the mean behavior of ten flies. Intestinal transit was measured in flies given brief access (30 min) to dye-colored food, with dye location in the intestine determined visually. A transit index was calculated based on the leading dye edge position, with scores of 1, 2, and 3 referring to dye edge in the crop/anterior midgut, middle midgut, and hindgut/anus, respectively.

Sparse neuronal labeling

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Piezo-Gal4 (59266) flies were crossed with MultiColor FlpOut (MCFO) flies (Hs-Flp; UAS-MCFO flies) that enable multicolor, stochastic, and sparse labeling of Gal4-expressing cells (Nern et al., 2015). MCFO flies contain multiple Gal4-dependent alleles encoding epitope tags, including HA, FLAG, and V5. Piezo-Gal4; Hs-Flp; UAS-MCFO fly larvae were maintained at 19°C, and at the third instar, larvae (wandering stage) were heat-shocked (37°C, 15 min/day, 3 days) to induce reporter expression in dispersed neurons, and after eclosion, were collected for dissection of the brain and anterior gut and immunohistochemistry for HA, Flag, and V5 epitopes.

Immunohistochemistry

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Wholemount preparations of the gastrointestinal tract and brain were fixed (4% paraformaldehyde, phosphate buffered saline or PBS, 20 min, room temperature [RT]), washed (2 × 5 min, PBS with 0.5% Triton X-100), permeabilized (10 min, PBS with 0.5% Triton X-100), blocked (1 hr, RT, blocking solution: 5% normal goat serum [Jackson ImmunoResearch, 005-000-121], PBS with 0.1% Triton X-100), incubated with primary antibody (1:200, blocking solution, 4°C, overnight), washed (3 × 10 min, RT, PBS with 0.1% Triton X-100), incubated with secondary antibody (1:200, PBS with 0.1% Triton X-100, 2 hr, RT), washed (3 × 10 min, RT, PBS with 0.1% Triton X-100 then 2 × 5 min, RT, PBS), mounted on a slide glass with Fluoromount-G mounting medium (Southern Biotech, 0100-01), covered with a thin coverslip, sealed with nail polish, and analyzed by confocal microscopy (Leica SP5). Primary antibodies were anti-GFP (Thermo Fisher Scientific, Chicken, A10262), anti-RFP (Rockland, Rabbit, 600-401-379), anti-Elav (Developmental Studies Hydridoma Bank, Mouse, Elav-9F8A9), anti-Akh (Kerafast, Rabbit, EGA261), anti-Dilp2 (from Veenstra Jan, University of Bordeaux, France), anti-Flag (Novus Biologicals, Rat, NBP1-06712SS), anti-HA (Cell Signaling Technology, Rabbit, 3724S), and anti-V5 (Bio-Rad, Mouse, MCA2894D549GA). Secondary antibodies were anti-Chicken-Alexa Fluor-488 (Jackson ImmunoResearch, 703-545-155), anti-Rabbit-Alexa Fluor-488 (Jackson ImmunoResearch, 711-545-152), anti-Rabbit-Cy3 (Jackson ImmunoResearch, 711-165-152), anti-Rabbit-Alexa Fluor-647 (Jackson ImmunoResearch, 711-605-152), anti-Mouse-Alexa Fluor-647 (Jackson ImmunoResearch, 715-605-150), anti-Mouse-Alexa Flour-488 (Jackson ImmunoResearch, 715-545-150), and anti-Rat-Alexa Fluor 488 (Jackson ImmunoResearch, 712-545-153). For staining of visceral muscle and nuclei, Phalloidin-FITC (Sigma, P5282-1MG), Phalloidin-TRITC (Sigma, P1951-1MG), and TO-PRO-3 (ThermoFisher, T3605) were added together with the secondary antibody.

Quantification of crop size and composition

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After the feeding assay, flies were fixed (4% paraformaldehyde, PBS, RT, 1 hr) and decapitated. The anterior gastrointestinal tract was surgically removed after gentle displacement of appendages and thoracic muscles. Dissected tissue was washed (3× PBS, RT, 5 min) and mounted for bright-field microscopy using the ‘Analyze-Measure’ tool in Fiji to calculate crop area. Crop muscle and cell density were quantified as detailed below. For quantification of crop muscle density, the intensity of the Phalloidin-labeled muscle fibers in a region of interest (ROI) was divided by the total ROI area. For cell density, the number of nuclei labeled with TO-PRO-3 and counted using ‘Analyse-3D objects counter’ function in Fiji (https://imagej.net/Fiji) was divided by total ROI area.

Analyzing neuronal responses with CaLexA

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CaLexA responses were measured in Piezo-Gal4 or Gr43a-Gal4 flies containing UAS-CaLexA (LexA-VP16-NFAT, LexAop-rCD2-GFP, and LexAop-CD8GFP-2A-CD8GFP), and UAS-CD8RFP. Responses of Piezo knockout neurons were measured by introducing Piezo knockout alleles into Piezo-Gal4; UAS-CaLexA; UAS-CD8RFP flies. For sucrose and sucralose responses, flies were fed ad libitum with regular food, transferred to vials containing a kimwipe soaked with 10% sucrose solution or 1% sucralose solution containing green food coloring for 24 hr, and analyzed for crop distension and CaLexA expression. For water responses, flies were deprived of food and water for 6 hr, and transferred to vials containing a water-soaked kimwipe. Some flies were harvested after 15 min for analysis of crop distension and others were harvested after 18 hr for analysis of CaLexA expression. Control flies were placed in a vial containing a water-soaked kimwipe but no food for 24 hr and harvested for analysis. For TrpA1-mediated neuron stimulation, WT and Piezo KO flies bearing a Piezo-Gal4, UAS-CaLexA (LexA-VP16-NFAT, LexAop-rCD2GFP, and LexAop-CD8GFP-2A-CD8GFP), and UAS-Trpa1 were placed in a 30°C incubator for 24 hr prior to analysis. For analysis of CaLexA expression, flies were anesthetized (ice, 10 min), and the anterior gastrointestinal tract was surgically removed. Dissected tissue was fixed (4% paraformaldehyde, PBS, 20 min, RT), washed (3 × 5 min, PBS), and slide mounted with Fluoromount-G mounting medium and a coverslip. Native GFP (derived from CaLexA activation) and RFP (constitutive from a Gal4-dependent reporter) fluorescence was analyzed by confocal microscopy (Leica SP5).

For quantification of CaLexA-dependent reporter in Figure 3B and S3B, intensity of GFP and RFP fluorescence was calculated per neuron and a CaLexA index expressed as GFP fluorescence divided by RFP fluorescence. For quantification of CaLexA-dependent reporter in Figure 2—figure supplement 2A, D, which involved flies lacking an RFP allele for neuron identification and normalization, GFP intensity was measured in the whole hypocerebral ganglion and a background subtraction was performed involving a comparably sized region of the proventriculus lacking Gal4-positive cell bodies. For S3A and S3D, background-subtracted GFP fluorescence was divided by RFP fluorescence from a control Piezo-Gal4; UAS-CD8RFP fly to generate a CaLexA index.

Statistical analysis

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Data in graphs are represented as means ± SEM, with sample sizes provided in figure legends. Statistical significance was analyzed by ANOVA Dunnett’s multiple comparison test or unpaired t-test using Prism 8 software (GraphPad), as indicated in figure legends.

Data availability

All datapoints used are provided in figures and in a source data file.

References

    1. Dethier VG
    2. Gelperin A
    (1967)
    Hyperphagia in the blowfly
    The Journal of Experimental Biology 191:200.
    1. Montell C
    (2005) Drosophila TRP channels
    Pflügers Archiv - European Journal of Physiology 451:19–28.
    https://doi.org/10.1007/s00424-005-1426-2
    1. Phillips RJ
    2. Powley TL
    (1996) Gastric volume rather than nutrient content inhibits food intake
    American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 271:R766–R769.
    https://doi.org/10.1152/ajpregu.1996.271.3.R766

Decision letter

  1. Claude Desplan
    Reviewing Editor; New York University, United States
  2. Piali Sengupta
    Senior Editor; Brandeis University, United States

In the interests of transparency, eLife publishes the most substantive revision requests and the accompanying author responses.

Acceptance summary:

The paper elegantly demonstrates that the mechanosensor Piezo is important in the gut for controlling meal size. It also describes the circuits that control this behavior. The requirement for Piezo in the hypocerebral ganglion to detect enlargement of the crop provides an overall mechanism for the regulation.

Decision letter after peer review:

Thank you for submitting your article "Control of feeding by Piezo-mediated gut mechanosensation in Drosophila" for consideration by eLife. Your article has been reviewed by three peer reviewers, and the evaluation has been overseen by a Reviewing Editor and Piali Sengupta as the Senior Editor. The reviewers have opted to remain anonymous.

The three reviewers have discussed the reviews with one another and the Reviewing Editor has drafted this decision to help you prepare a revised submission.

We would like to draw your attention to changes in our revision policy that we have made in response to COVID-19 (https://elifesciences.org/articles/57162). Specifically, when editors judge that a submitted work as a whole belongs in eLife but that some conclusions require a modest amount of additional new data, as they do with your paper, we are asking that the manuscript be revised to either limit claims to those supported by data in hand, or to explicitly state that the relevant conclusions require additional supporting data.

Our expectation is that the authors will eventually carry out the additional experiments and report on how they affect the relevant conclusions either in a preprint on bioRxiv or medRxiv, or if appropriate, as a Research Advance in eLife, either of which would be linked to the original paper.

The three reviewers appreciated the significance of your work and the elegance of the manuscript with the discovery of Piezo in neurons that control internal feeding. The reviewers are therefore positive but would like you to clarify three points that you should be able to complete in the coming weeks.

The paper would benefit with a better identification of the neurons involved, especially, the localization of the cell bodies and the axons. This would help readers better appreciate the work, given that another paper has already described the same neurons.

Therefore, we would like you to:

1) Clarify which subset of Piezo-positive neurons are responsible for innervating the crop vs. the anterior midgut;

2) Determine to what extent Cha-Gal80 inhibits Pz-Gal4 expression in the Dilp2-Gal4 neurons;

3) Clarify whether Dilp2-Gal4 is expressed in the HCG neurons.

We realize that the first point might require work that would exceed a reasonable timeframe, especially given the relative urgency of the situation, but you might already have these experiments in hand. Points #2 and 3 should easily be done.

Reviewer #1:

Gut mechanosensation is critical for controlling meal size, but the molecules and circuits responsible have not been described in detail. Working in Drosophila, the authors show that the evolutionarily conserved mechanosensor Piezo is important for controlling meal size. Piezo mutants are found to over-consume when feeding, with the crop becoming distended, and Piezo is shown to be required for a subset of Piezo+ neurons in the hypocerebral ganglion (HCG) to become activated post-feeding, presumably responding to inflation of the crop. Overall, the study is well-performed and the conclusions are careful and largely justified by the data presented. The only potential weak spot is the assignment of Piezo's behavioral functions to the Piezo(+) neurons in the HCG, but the authors do note this. Overall, Identifying Piezo's role in feeding control is a significant advance.

The behavioral experiments and CaLexA studies are overall well-performed and support the conclusions drawn.

The identity of the Piezo-positive neurons responsible for innervating the crop vs. the anterior midgut should be clarified. The Piezo-positive neurons in the HCG respond to feeding, but it is difficult to tell whether these neurons innervate the anterior midgut, the crop or both structures. A clearer view of what this subset of Pz(+) neurons innervate would be useful in thinking about the mechanism at work. Perhaps a FLP-out stochastic labeling experiment or an intersectional labeling experiment could help clarify this issue.

Clarifying the extent to which Cha-Gal80 blocks Pz-Gal4 activity in the brain would be useful, as descending neurons could also contribute to the behavioral effects observed. In the main figure, it appears that Cha-Gal80 eliminates Pz-Gal4-driven RFP expression throughout the brain, but in Figure 2—figure supplement 1 it appears that the IPC is spared. This is particularly interesting as the companion paper from Wang et al., 2020, identifies the Dilp2-Gal4 neurons of the IPC rather than the HCG as Piezo's site of action in feeding control. It would be helpful to know: 1) to what extent Cha-Gal80 inhibits Pz-Gal4 expression in the Dilp2-Gal4 neurons and 2) whether Dilp2-Gal4 is expressed in the HCG neurons. These experiments should be straightforward to perform and would help provide a sense of the relative importance of HCG neurons vs. Dilp2 neurons in controlling feeding behavior.

Reviewer #2:

In the 70th, Dethier and Gelperin described mechanosensory nerves innervating the crop in large flies, which induced hyperphagia after transecting them. In this work, Min et al. demonstrate convincingly that similar neurons are present in Drosophila, which express a gene called Piezo. These stretch receptors innervate the crop and the anterior midgut of the digestive system of adult flies. The cells expressing Piezo are distinct from cells expressing genes involved in meal termination like gustatory receptors (Gr43a), the receptor to the adipokinetic hormone (Akh), and also distinct from other cells innervating the gut (like GMR41F12). Using genetic constructions targeting Piezo neurons that activate them (heat activated TRPA1 inducing action potentials) or inhibit them (temperature-sensitive Shibire blocking synaptic transmission at permissive temperatures), they show these neurons induce a cessation of the feeding activities. Piezo neurons thus modulate the volume of food ingested and more precisely, the volume stored into the crop. Flies with a deficient Piezo gene (KO), are hyperphagic, with an enormously distended crop, while flies in which this gene is rescued are comparable to control flies. Additional observations using a CaLexA reporter system confirm that feeding induce a functional activation of these neurons, and that these Piezo neurons are involved in sensing the state of distension of the crop. This work provides a solid foundation to a better understanding of the mechanosensory neurons that allow flies to control the volume of liquids ingested.

Reviewer #3:

This paper investigates the mechanism by which mechanosensory neurons that project to the gut sense that it is full of food. Using reporter lines labeling cells expressing various mechanotransduction channels, they identified expression of Piezo in hypocerebral ganglion neurons with mechanosensory endings in the gut. They show that silencing Piezo-expressing neurons promoted feeding while activating them inhibited feeding. Moreover, they showed using the CaLexA reporter that stimuli that distend the gut increased the activity of Piezo-expressing neurons, and that this activation was lost in Piezo knockout flies. The Piezo knockout mutants also showed behavioral abnormalities indicating that they no longer stop feeding when their gut is full. This evidence suggests Piezo is the mechanotransducer in the hypocerebral ganglion neurons which senses a distended gut.

Overall this is an interesting story that expands the range of functions for Piezo, and thus is of significant interest to the mechanotransduction field. There are places where ideally one would have wanted to see more precise genetic definition of the cellular site of action for Piezo (i.e. based on the authors' interpretations, in the hypocerebral ganglion neurons); I assume this is due to a lack of specific driver lines, time pressure from the competing manuscript or both (I am not a fly person so I don't know whether more specific drivers even exist). I think the authors are pretty clear about the evidence and don't overstate things, so I am happy for the paper to be published in this form.

https://doi.org/10.7554/eLife.63049.sa1

Author response

The three reviewers appreciated the significance of your work and the elegance of the manuscript with the discovery of Piezo in neurons that control internal feeding. The reviewers are therefore positive but would like you to clarify three points that you should be able to complete in the coming weeks.

The paper would benefit with a better identification of the neurons involved, especially, the localization of the cell bodies and the axons. This would help readers better appreciate the work, given that another paper has already described the same neurons.

Therefore, we would like you to:

1) Clarify which subset of Piezo-positive neurons are responsible for innervating the crop vs. the anterior midgut;

We added new data involving sparse labeling of Piezo-expressing cells to distinguish the innervation patterns of HCG neurons and Dilp2 neurons of the pars intercerebralis, which is the location of gut-innervating Piezo neurons reported in Wang et al., 2020. Briefly, we crossed Piezo-Gal4 flies with MultiColor FlpOut (MCFO) flies (Hs-Flp; UAS-MCFO flies) that enable multicolor, stochastic, and sparse labeling of Gal4-expressing cells (Nern et al., 2015). We obtained several flies with expression in HCG neurons but not in the pars intercerebralis, and in these flies, we still observed fibers in the crop and anterior midgut, with individual neurons capable of displaying selective innervation of one of these targets. These new data are presented in Figure 1—figure supplement 1C and clearly indicate that Piezo neurons outside of the pars intercerebralis innervate the anterior gut. Further supporting this idea, we have other new data from Piezo-Gal4; Cha-Gal80 flies (please see response to point #2) that provide similar results. All of these anatomical findings are consistent with our observations that HCG Piezo neurons respond to gut distension in a Piezo-dependent manner. These findings, when taken together with the recent Wang et al., 2020 paper, suggest a model of multiple gut-innervating Piezo neuron subtypes.

2) Determine to what extent Cha-Gal80 inhibits Pz-Gal4 expression in the Dilp2-Gal4 neurons;

We performed additional experiments to quantify Gal4-driven reporter expression in various neurons of Piezo-Gal4; UAS-CD8RFP; Cha-Gal80 flies and Piezo-Gal4; UAS-CD8RFP flies. In control Piezo-Gal4; UAS-CD8RFP flies, we observed reporter expression per fly in 6.2 ± 0.5 HCG neurons and 4.9 ± 1.0 π neurons, 2.9 ± 0.7 of which express Dilp2. In Piezo-Gal4; UAS-CD8RFP; Cha-Gal80 flies, we observed reporter expression per fly in 5.2 ± 0.5 HCG neurons and 1.1 ± 0.5 π neuron, 0.4 ± 0.3 of which express Dilp2 (about half of flies had one co-labeled neuron and half had zero). In flies that lack any reporter expression in pars intercerebralis Dilp2 neurons, we still observed labeled neurites in the anterior midgut and crop nerve, consistent with findings from stochastic labeling detailed above that neurons outside of the pars intercerebralis innervate these regions. These new findings are presented in Figure 2—figure supplement 3B and C. Furthermore, despite rare labeling of pars intercerebralis Dilp2 neurons in Piezo-Gal4; Cha-Gal80 flies, activation of labeled neurons still inhibited feeding. These findings, as above, are consistent with a model of multiple gut-innervating Piezo neuron subtypes.

3) Clarify whether Dilp2-Gal4 is expressed in the HCG neurons.

Dilp2-Gal4 does not drive reporter expression to HCG neurons (stained by Elav). We observed Dilp2-labeled fibers that pass through the HCG ganglion but not Dilp2-labeled HCG soma; these findings are presented in Figure 2—figure supplement 3D.

We realize that the first point might require work that would exceed a reasonable timeframe, especially given the relative urgency of the situation, but you might already have these experiments in hand. Points #2 and 3 should easily be done.

Reviewer #1:

Gut mechanosensation is critical for controlling meal size, but the molecules and circuits responsible have not been described in detail. Working in Drosophila, the authors show that the evolutionarily conserved mechanosensor Piezo is important for controlling meal size. Piezo mutants are found to over-consume when feeding, with the crop becoming distended, and Piezo is shown to be required for a subset of Piezo+ neurons in the hypocerebral ganglion (HCG) to become activated post-feeding, presumably responding to inflation of the crop. Overall, the study is well-performed and the conclusions are careful and largely justified by the data presented. The only potential weak spot is the assignment of Piezo's behavioral functions to the Piezo(+) neurons in the HCG, but the authors do note this. Overall, Identifying Piezo's role in feeding control is a significant advance.

The behavioral experiments and CaLexA studies are overall well-performed and support the conclusions drawn.

The identity of the Piezo-positive neurons responsible for innervating the crop vs. the anterior midgut should be clarified. The Piezo-positive neurons in the HCG respond to feeding, but it is difficult to tell whether these neurons innervate the anterior midgut, the crop or both structures. A clearer view of what this subset of Pz(+) neurons innervate would be useful in thinking about the mechanism at work. Perhaps a FLP-out stochastic labeling experiment or an intersectional labeling experiment could help clarify this issue.

Thank you for thoughts on the paper. We performed the stochastic labeling experiments requested, which have provided a nice additional dataset. As described above, we added new data involving sparse labeling of Piezo-expressing cells to distinguish the innervation patterns of HCG neurons and Dilp2 neurons of the pars intercerebralis, which is the location of gut-innervating Piezo neurons reported in Wang et al., 2020. Briefly, we crossed Piezo-Gal4 flies with MultiColor FlpOut (MCFO) flies (Hs-Flp; UAS-MCFO flies) that enable multicolor, stochastic, and sparse labeling of Gal4-expressing cells (Nern et al., 2015). We obtained several flies with expression in HCG neurons but not in the pars intercerebralis, and in these flies, we still observed fibers in the crop and anterior midgut, with individual neurons capable of displaying selective innervation of one of these targets. These new data are presented in Figure 1—figure supplement 1C, and clearly indicate that Piezo neurons outside of the pars intercerebralis innervate the anterior gut. Further supporting this idea, we have other new data from Piezo-Gal4; Cha-Gal80 flies (please see response to point #2) that provide similar results. All of these anatomical findings are consistent with our observations that HCG Piezo neurons respond to gut distension in a Piezo-dependent manner. These findings, when taken together with the recent Wang et al., 2020 paper, suggest a model of multiple gut-innervating Piezo neuron subtypes.

Clarifying the extent to which Cha-Gal80 blocks Pz-Gal4 activity in the brain would be useful, as descending neurons could also contribute to the behavioral effects observed. In the main figure, it appears that Cha-Gal80 eliminates Pz-Gal4-driven RFP expression throughout the brain, but in Figure 2—figure supplement 1 it appears that the IPC is spared. This is particularly interesting as the companion paper from Wang et al., 2020, identifies the Dilp2-Gal4 neurons of the IPC rather than the HCG as Piezo's site of action in feeding control. It would be helpful to know: 1) to what extent Cha-Gal80 inhibits Pz-Gal4 expression in the Dilp2-Gal4 neurons and 2) whether Dilp2-Gal4 is expressed in the HCG neurons. These experiments should be straightforward to perform and would help provide a sense of the relative importance of HCG neurons vs. Dilp2 neurons in controlling feeding behavior.

We have performed both experiments requested. As described above, we first performed additional experiments to quantify Gal4-driven reporter expression in central and peripheral neurons of Piezo-Gal4; UAS-CD8RFP; Cha-Gal80 flies and Piezo-Gal4; UAS-CD8RFP flies. In control Piezo-Gal4; UAS-CD8RFP flies, we observed reporter expression per fly in 6.2 ± 0.5 HCG neurons and 4.9 ± 1.0 π neurons, 2.9 ± 0.7 of which express Dilp2. In Piezo-Gal4; UAS-CD8RFP; Cha-Gal80 flies, we observed reporter expression per fly in 5.2 ± 0.5 HCG neurons and 1.1 ± 0.5 π neuron, 0.4 ± 0.3 of which express Dilp2 (about half of flies of one co-labeled neuron and half have zero). In flies that lack any reporter expression in pars intercerebralis Dilp2 neurons, we still observed labeled neurites in the anterior midgut and crop nerve, consistent with findings from stochastic labeling detailed above that neurons outside of the pars intercerebralis innervate these regions. These new findings are presented in Figure 2—figure supplement 3B and C. Furthermore, despite rare labeling of pars intercerebralis Dilp2 neurons in Piezo-Gal4; Cha-Gal80 flies, activation of labeled neurons still inhibited feeding. We also performed experiments that show that Dilp2-Gal4 does not drive reporter expression to HCG neurons (stained by Elav). We observed Dilp2 fibers that pass through the HCG ganglion but not Dilp2-labeled HCG soma; these findings are presented in Figure 2—figure supplement 3D. Together, these findings are further consistent with a model of multiple gut-innervating Piezo neuron subtypes.

Reviewer #3:

This paper investigates the mechanism by which mechanosensory neurons that project to the gut sense that it is full of food. Using reporter lines labeling cells expressing various mechanotransduction channels, they identified expression of Piezo in hypocerebral ganglion neurons with mechanosensory endings in the gut. They show that silencing Piezo-expressing neurons promoted feeding while activating them inhibited feeding. Moreover, they showed using the CaLexA reporter that stimuli that distend the gut increased the activity of Piezo-expressing neurons, and that this activation was lost in Piezo knockout flies. The Piezo knockout mutants also showed behavioral abnormalities indicating that they no longer stop feeding when their gut is full. This evidence suggests Piezo is the mechanotransducer in the hypocerebral ganglion neurons which senses a distended gut.

Overall this is an interesting story that expands the range of functions for Piezo, and thus is of significant interest to the mechanotransduction field. There are places where ideally one would have wanted to see more precise genetic definition of the cellular site of action for Piezo (i.e. based on the authors' interpretations, in the hypocerebral ganglion neurons); I assume this is due to a lack of specific driver lines, time pressure from the competing manuscript or both (I am not a fly person so I don't know whether more specific drivers even exist). I think the authors are pretty clear about the evidence and don't overstate things, so I am happy for the paper to be published in this form.

Thank you for taking the time to review the paper and for your supportive comments. We have provided additional experiments, as detailed above, that show the extent of specificity in our genetic experiments involving Cha-Gal80, and suggest the model that distinct Piezo neuron subtypes contribute to feeding control.

https://doi.org/10.7554/eLife.63049.sa2

Article and author information

Author details

  1. Soohong Min

    Howard Hughes Medical Institute, Harvard Medical School, Department of Cell Biology, Boston, United States
    Contribution
    Conceptualization, Data curation, Formal analysis, Funding acquisition, Validation, Investigation, Visualization, Methodology, Writing - original draft, Writing - review and editing
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-0683-2935
  2. Yangkyun Oh

    Skirball Institute, NYU School of Medicine, New York, United States
    Contribution
    Data curation, Formal analysis, Investigation, Visualization, Methodology
    Competing interests
    No competing interests declared
  3. Pushpa Verma

    Harvard Medical School, Department of Cell Biology, Boston, United States
    Contribution
    Resources
    Competing interests
    No competing interests declared
  4. Samuel C Whitehead

    Department of Physics, Cornell University, Ithaca, United States
    Contribution
    Resources, Software, Funding acquisition
    Competing interests
    No competing interests declared
  5. Nilay Yapici

    Department of Neurobiology and Behavior, Cornell University, Ithaca, United States
    Contribution
    Conceptualization, Resources, Software, Supervision, Funding acquisition, Project administration, Writing - review and editing
    Competing interests
    No competing interests declared
  6. David Van Vactor

    Harvard Medical School, Department of Cell Biology, Boston, United States
    Contribution
    Conceptualization, Resources, Supervision, Funding acquisition, Writing - original draft, Project administration, Writing - review and editing
    Competing interests
    No competing interests declared
  7. Greg SB Suh

    1. Skirball Institute, NYU School of Medicine, New York, United States
    2. KAIST, Department of Biological Sciences, Daejeon, Republic of Korea
    Contribution
    Conceptualization, Resources, Software, Supervision, Funding acquisition, Project administration, Writing - review and editing
    Competing interests
    No competing interests declared
  8. Stephen Liberles

    Howard Hughes Medical Institute, Harvard Medical School, Department of Cell Biology, Boston, United States
    Contribution
    Conceptualization, Resources, Software, Supervision, Funding acquisition, Writing - original draft, Project administration, Writing - review and editing
    For correspondence
    Stephen_Liberles@hms.harvard.edu
    Competing interests
    Reviewing editor, eLife
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-2177-9741

Funding

American Heart Association (20POST35210914)

  • Soohong Min

National Institutes of Health (NS090994)

  • David Van Vactor

National Institutes of Health (RO1DK116294)

  • Greg SB Suh

National Institutes of Health (RO1DK106636)

  • Greg SB Suh

Samsung Science and Technology Foundation (SSTF-BA-1802-11)

  • Greg SB Suh

Howard Hughes Medical Institute

  • Stephen Liberles

National Institutes of Health (R35 GM133698-01)

  • Nilay Yapici

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Acknowledgements

We thank Norbert Perrimon, Veenstra Jan, Bryan Song, and Dragana Rogulja for reagents and advice, Jinfei Ni for blinded analysis of behavior, Norbert Perrimon, Craig Montell, Julie Simpson, Hubert Amrein, and Bloomington Drosophila Stock Center for flies, and Hansine Heggeness and Exelixis facility at Harvard Medical School for fly food and stock maintenance.

Senior Editor

  1. Piali Sengupta, Brandeis University, United States

Reviewing Editor

  1. Claude Desplan, New York University, United States

Publication history

  1. Received: September 11, 2020
  2. Accepted: February 16, 2021
  3. Accepted Manuscript published: February 18, 2021 (version 1)
  4. Version of Record published: March 1, 2021 (version 2)

Copyright

© 2021, Min et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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