Investigation of Drosophila fruitless neurons that express Dpr/DIP cell adhesion molecules
- Florida State University, United States
- Baylor College of Medicine, United States
- California Institute of Technology, United States
Abstract
Drosophila reproductive behaviors are directed by fruitless neurons. A reanalysis of genomic studies shows that genes encoding dpr and DIP Immunoglobulin superfamily (IgSF) members are expressed in fru P1 neurons. We find that each fru P1 and dpr/DIP (fru P1 ∩ dpr/DIP) overlapping expression pattern is similar in both sexes, but there are dimorphisms in neuronal morphology and cell number. Behavioral studies of fru P1 ∩ dpr/DIP perturbation genotypes indicates that the mushroom body functions together with the lateral protocerebral complex to direct courtship behavior. A single-cell RNA-seq analysis of fru P1 neurons shows that many DIPs have high expression in a small set of neurons, whereas the dprs are often expressed in a larger set of neurons at intermediate levels, with a myriad of dpr/DIP expression combinations. Functionally, we find that perturbations of sex hierarchy genes and of DIP-ε change the sex-specific morphologies of fru P1 ∩ DIP-α neurons.
Data availability
All raw data are provided in the supplementary materials. The sequencing data has been deposited in GEO under accession number GSE162098
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Investigation of Drosophila fruitless neurons that express Dpr/DIP cell adhesion moleculesNCBI Gene Expression Omnibus, GSE162098.
Article and author information
Author details
Michelle N Arbeitman
Funding
National Institutes of Health (R01 grant number R01GM073039)
- Savannah G Brovero
- Julia C Fortier
- Hongru Hu
- Pamela C Lovejoy
- Nicole R Newell
- Colleen M Palmateer
- Ruei-Ying Tzeng
- Michelle N Arbeitman
National Institutes of Health (R03 grant number R03NS090184)
- Ruei-Ying Tzeng
National Institutes of Health (R01 grant number R01GM073039)
- Michelle N Arbeitman
Florida State University (R01 grant number Biomedical Sciences)
- Hongru Hu
- Pamela C Lovejoy
- Nicole R Newell
- Colleen M Palmateer
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Reviewing Editor
- Michael B Eisen, University of California, Berkeley, United States
Version history
- Received: September 17, 2020
- Accepted: February 22, 2021
- Accepted Manuscript published: February 22, 2021 (version 1)
- Version of Record published: March 18, 2021 (version 2)
Copyright
© 2021, Brovero et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Investigation of Drosophila fruitless neurons that express Dpr/DIP cell adhesion molecules
eLife 10:e63101.
https://doi.org/10.7554/eLife.63101
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