scAAVengr, a transcriptome-based pipeline for quantitative ranking of engineered AAVs with single-cell resolution

AAV selection rounds in canines 2b and 5b were repeated selections of the previous rounds, which did not result in the amplification of AAV variants.

A. Friedman’s test was conducted to determine differences across AAV variants. The test was run separately for each cell type as well as total cells combined. Marmoset and cynomolgus macaque samples were both used in the analysis (n = 8). Significant P-values < 0.05 are shown in bold red. S2.1 p-values resulting from a Friedman’s test using percent cells infected as the data points. Significant P-values < 0.05 are shown in bold red. S2.2 p-values resulting from a Friedman’s test using average transcripts per infected cell as the data points. Significant P-values < 0.05 are shown in bold red.

A one-sided Wilcoxon signed-rank test was used for a pairwise comparison between K912 or NHP26 and the other variants. P-values were corrected using Benjamini-Hochberg correction method. Significant P-values < 0.05 are shown in bold red. S3.1 p-values resulting from a one-sided Wilcoxon signed-rank test using percent cells infected as the data points, comparing NHP12 and other variants. S3.2 p-values resulting from a one-sided Wilcoxon signed-rank test using percent cells infected as the data points, comparing NHP26 and other variants. S3.3 p-values resulting from a one-sided Wilcoxon signed-rank test using average transcripts per infected cell as the data points, comparing K912 and other variants. S3.4 p-values resulting from a one-sided Wilcoxon signed-rank test using average transcripts per infected cell as the data points, comparing NHP26 and other variants.