The causal role of auditory cortex in auditory working memory
- Key Laboratory of Brain Functional Genomics (Ministry of Education and Shanghai), Key Laboratory of Adolescent Health Assessment and Exercise Intervention of Ministry of Education, and School of Life Sciences, East China Normal University, China
Figures
Figure 1 with 1 supplement
Auditory working memory task in head-fixed mice and behavioral performance.
(a) Diagram of the experimental setup. (b) Schematic for task design. For each trial, an auditory stimulus (3 kHz, or 12 kHz, 0.2 s) was presented as the sample, followed by a delay period of 1.5 s …
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Figure 1—source data 1
Performance in the auditory working memory task.
- https://cdn.elifesciences.org/articles/64457/elife-64457-fig1-data1-v2.xlsx
Figure 1—figure supplement 1
The learning process of the auditory working memory (WM) task and the licking behavior of well-trained mice.
(a) Performances of all mice while learning the auditory WM task of 1.5 s delay. (b–f) Averaged licking rate in hit (left) and correct rejection (right) trials of mice well trained for the WM task …
Figure 2 with 1 supplement
Neural correlates of the auditory cortex activity in the auditory working memory (WM) task.
(a) Raster (top) and peri-stimulus time histograms (bottom) of an example neuron recorded during the WM behavior (left) and passive listening (right). Trials were sorted by auditory samples. The …
Figure 2—figure supplement 1
Neural correlates of the auditory cortex activity in the auditory working memory (WM) task with varied stimulus duration.
(a, b) Averaged population firing rates for neurons recorded during auditory WM behavior with the stimulus duration of 300 ms (a) and 400 ms (b). The black block on the top indicates the successive …
Figure 3 with 1 supplement
Suppression of delay-period activity in auditory cortex (AC) by optogenetic inhibition of pyramidal neurons impaired auditory working memory (WM) performance.
(a) Histology image showing the expression of AAV-CaMKIIα-eNpHR3.0-eYFP in AC. (b) Activity suppression efficiency revealed by optetrode recording in vivo. (c) Suppressing AC activity during the …
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Figure 3—source data 1
Effect of auditory cortex suppression on working memory behavior.
- https://cdn.elifesciences.org/articles/64457/elife-64457-fig3-data1-v2.xlsx
Figure 3—figure supplement 1
Optogenetic suppression of auditory cortex during the stimulus epoch dramatically reduced the animals’ ability to perform the working memory task.
Figure 4
Temporal specificity of the effect of auditory cortex (AC) suppression.
(a) AC suppression during the delay period of 300–800 ms decreased the working memory (WM) behavioral performance, with an increase in false alarm (FA) rate and no change in hit rate. (b) AC …
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Figure 4—source data 1
Effect of auditory cortex suppression during delay period of 300–800 ms and 800–1500 ms.
- https://cdn.elifesciences.org/articles/64457/elife-64457-fig4-data1-v2.xlsx
Figure 5
Performance was impaired following optogenetic suppression of auditory cortex (AC) activity during the early delay period, with the delay duration of 3 s and 7 s.
(a, b) In working memory (WM) task with the delay duration of 3 s, AC suppression during the early delay period (300–800 ms) (a) but not later (800–2700 ms) (b) decreased the behavioral performance. …
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Figure 5—source data 1
Effect of auditory cortex suppression in working memory task with the delay duration of 3 s and 7 s.
- https://cdn.elifesciences.org/articles/64457/elife-64457-fig5-data1-v2.xlsx
Figure 6
Active memory maintenance in auditory working memory (WM) task by the auditory cortex (AC) delay-period activity.
(a) Learning curve for the performance in the WM task with noise distractor (presented during 300–500 ms of the delay period). Note the drop of performance after inserting the noise distractor in …
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Figure 6—source data 1
Effect of auditory cortex suppression in working memory task with noise distractor.
- https://cdn.elifesciences.org/articles/64457/elife-64457-fig6-data1-v2.xlsx
Figure 7
Suppressing auditory cortex (AC) activity did not affect behavioral performance in the delayed go/no-go auditory discrimination task and go/no-go auditory discrimination task.
(a) Paradigm and behavioral performance for the delayed go/no-go auditory discrimination task experiments with suppressed AC activity. (b) As in (a) for the go/no-go auditory discrimination task. …
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Figure 7—source data 1
Effect of auditory cortex suppression in delayed go/no-go auditory discrimination task and go/no-go auditory discrimination task.
- https://cdn.elifesciences.org/articles/64457/elife-64457-fig7-data1-v2.xlsx
Tables
Key resources table
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | C57BL/6 | Slac Laboratory Animal | N/A | |
| Other | Formvar-Insulated Nichrome Wire | A-M Systems | 761000 | |
| Other | Head-stage amplifier | Intan Technology | RHD2132 | |
| Software, algorithm | MATLAB | MathWorks | SCR-001622 |
