Galectin-9 regulates the threshold of B cell activation and autoimmunity

Acceptance summary:

By using galectin 9-KO mouse, authors nicely showed that galectin-9 plays a critical role in setting the threshold of B cell activation. Furthermore, they demonstrated that galectin-9 regulates the interaction between BCR and TLRs with CD5 and CD180. These findings well explain why loss of galectin-9 drives autoimmune responses.

Decision letter after peer review:

Thank you for submitting your article "Galectin-9 regulates the threshold of B cell activation and autoimmunity" for consideration by eLife. Your article has been reviewed by 2 peer reviewers, one of whom is a member of our Board of Reviewing Editors, and the evaluation has been overseen by Carla Rothlin as the Senior Editor. The reviewers have opted to remain anonymous.

The reviewers have discussed the reviews with one another and the Reviewing Editor has drafted this decision to help you prepare a revised submission.

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Summary:

The authors studied the role of the soluble glycan-binding protein galectin-9 which can be produced by B cells and binds to B cell surface proteins such as membrane IgM and CD45. They used galectin-9 (Gal-9) KO mice to study the consequence of loss of this protein on B cell activation. They convincingly show that Gal-9 KO B cells react with higher BCR signaling and react to lower concentrations of antigens. This is true both for conventional B-2, as well as for B-1a cells. For B-1a cells not only BCR-induced signaling, but also TLR-signaling is enhanced. These studies on signaling, B cell activation and antigen internalisation are thoroughly done. For B-1a cells, direct binding of Gal-9 to CD5, TLR4 and CD180 is identified. Here some controls of other receptors and a discussion how this binding could happen would be useful. The authors also show that Gal-9 KO mice develop autoimmunity upon ageing or that in autoimmune models, the disease occurs more early. These parts are also generally done well. Overall it is an impressive amount of work with many interesting novel data.

Essential revisions:

It is important to clarify what is the cellular source of galectin-9 secretion in regard to development of autoimmunity in Gal9KO mice. Do B cells themselves secrete galectin-9 which binds to B cell surface to mediate signals, or is there any contribution of other cell types? Analysis of B cell-specific Gal9 KO mice (such as mixed BM chimera with B cell-deficient μMT mice) may help to address this question.